Effect of <i>Astragalus</i>  on Intestinal Alkaline Phosphatase and Inflammatory Response in Type 2 Diabetic Rats

WANG Yucong; XIE Zhixin; ZHANG Xueyan; YANG Qiuping; HAN Jianchun

doi:10.13386/j.issn1002-0306.2020080311

Volume 42 Issue 13

Jun. 2021

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Science and Technology of Food Industry > 2021 > 42(13): 351-357. > DOI: 10.13386/j.issn1002-0306.2020080311

WANG Yucong, XIE Zhixin, ZHANG Xueyan, et al. Effect of Astragalus on Intestinal Alkaline Phosphatase and Inflammatory Response in Type 2 Diabetic Rats[J]. Science and Technology of Food Industry, 2021, 42(13): 351−357. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020080311.

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Effect of Astragalus on Intestinal Alkaline Phosphatase and Inflammatory Response in Type 2 Diabetic Rats

1.
College of Food Science, Northeast Agricultural University, Harbin 150030, China
2.
Heilongjiang Green Food Science Research Institute, Harbin 150028, China
3.
Northeast Agricultural University Hospital, Harbin 150030, China

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Received Date: August 31, 2020
Available Online: April 29, 2021

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Abstract

Abstract

Objective: To explore the effects of Astragalus on alkaline phosphatase, bacterial endotoxin and serum inflammatory factors in type 2 diabetes rats. Methods: A high-sugar and high-fat diet + intraperitoneal injection of streptozotocin was used to establish a type 2 diabetes model. The rats were continuously administered Astragalus aqueous solution (0.25, 0.5 g/kg) and measureed the changes in body mass, blood sugar, the activity of intestinal alkaline phosphatase (IAP) in feces and the changes in bacterial endotoxin (LPS) concentration. MSD multi-factor method was used to detect serum inflammatory factors (Interleukin-6 (IL-6), Tumor necrosis factor-α (TNF-α), Interferon-γ (IFN-γ), Interleukin-1 (IL-1β), Interleukin-4 (IL-4), Interleukin-5 (IL-5), Interleukin-10 (IL-10), interleukin-13 (IL-13) growth regulatory oncogene α (KC/GRO)) concentration changes. Results: Compared with the model group, the fasting blood glucose concentration in the Astragalus dose group was significantly reduced (P<0.05), the IAP activity was significantly increased (P<0.05), the LPS concentration was significantly reduced (P<0.05), and the concentration of serum pro-inflammatory factors IL-6, TNF-α, IFN-γ and IL-1β were significantly reduced (P<0.05), while the concentration of anti-inflammatory factors IL-4, IL-5, IL-10, and IL-13 increased significantly (P<0.05), the concentration of chemokine KC/GRO decreased significantly (P<0.05). Conclusion: Astragalus could promote the activity of intestinal alkaline phosphatase and effectively regulate blood sugar and inflammation in T2DM mice.
- inflammation,
- diabetes,
- Astragalus,
- cytokine

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References

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