Culture Medium and Condition Optimization of <i>γ</i>-Polyglutamic Acid Synthesized by Microbial Fermentation Using Yellow Water

WANG Fengqing; BI Changfu; WANG Chuan; WANG Ning; GONG Lijuan; ZHOU Lihong; WANG Zhuqing

doi:10.13386/j.issn1002-0306.2020080014

Volume 42 Issue 11

May 2021

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Science and Technology of Food Industry > 2021 > 42(11): 106-115. > DOI: 10.13386/j.issn1002-0306.2020080014

WANG Fengqing, BI Changfu, WANG Chuan, et al. Culture Medium and Condition Optimization of γ -Polyglutamic Acid Synthesized by Microbial Fermentation Using Yellow Water[J]. Science and Technology of Food Industry, 2021, 42(11): 106−115. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020080014.

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Culture Medium and Condition Optimization of γ-Polyglutamic Acid Synthesized by Microbial Fermentation Using Yellow Water

College of Bioengineering, Sichuan University of Science & Engineering, Yibin 644004, China

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Received Date: August 03, 2020
Available Online: March 28, 2021

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Abstract

Yellow water, one of the by-products of Baijiu brewing. Microbial fermentation to produce γ-PGA using yellow water as substrate. This idea not only finds a new way for the comprehensive utilization of yellow water, but also realizes the high value utilization of yellow water. In this paper, the liquor by-product yellow water as the substrate for microbial fermentation, optimizing the culture medium and conditions of fermentation synthetic γ-PGA using single factor and response surface design experiments. Fermentation results were evaluated by measuring γ-PGA concentration, viscosity, residual glutamate and glucose concentration in fermentation broth. According to the results, when the pH value was 7.0 and the yellow water was diluted 7 times, Corn saccharification solution 75 g/L (by soluble solids content), Beef powder 70 g/L, Sodium glutamate 45 g/L, NaCl 12 g/L, K₂HPO₄ 7 g/L, the inoculation amount was 1%, 25 mL/250 mL, at 37 ℃ for 72 h, the yield of γ-PGA was 14.510 g/L. Hence, it is feasible to synthesize γ-PGA by microbial fermentation using yellow water as the substrate.
- Baijiu yellow water,
- γ-PGA,
- Bacillus subtilis,
- optimization of fermentation conditions,
- medium

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[1]	赵东, 牛广杰, 彭志云, 等. TA. XTplus 物性测试仪对黄水分析初探[J]. 酿酒,2013,40(3):28−30. doi: 10.3969/j.issn.1002-8110.2013.03.009
[2]	潘玲玲, 王媚, 罗明有, 等. 浓香型白酒黄水、窑泥中微生物总DNA提取方法比较[J]. 中国酿造,2016,35(4):42−46. doi: 10.11882/j.issn.0254-5071.2016.04.010
[3]	袁春红, 宋菲菲, 林凯, 等. 白酒发酵副产物黄水中两株产纤维素酶芽孢杆菌的分离鉴定[J]. 中国酿造,2014,33(11):90−93. doi: 10.11882/j.issn.0254-5071.2014.11.020
[4]	梁艳玲, 伍彦华, 苏芬芬, 等. 大曲酒副产物黄水的综合利用[J]. 轻工科技,2016(10):1−2, 20.
[5]	曹新莉, 王明山. 白酒生产中黄水和酒尾的应用[J]. 酿酒科技,2008(10):96−99.
[6]	王旭亮, 王德良, 韩兴林, 等. 白酒微生物研究与应用现状[J]. 酿酒科技,2009(6):88−91.
[7]	彭太升. 黄水调酒液在浓香型白酒中的应用[J]. 酿酒,2011,38(6):36−37. doi: 10.3969/j.issn.1002-8110.2011.06.016
[8]	Xinqiang TANG, Fenghua ZUO, Xinlu TANG, et al. Preparation of liquor flavoring from yellow water based on water absorbent resin[J]. Agricultural Biotechnology,2017,6(6):60−66.
[9]	张志刚, 何汝良, 程江红. 黄浆水酿醋工艺研究[J]. 中国酿造,2005(6):29−30. doi: 10.3969/j.issn.0254-5071.2005.06.009
[10]	周新虎, 陈翔, 王永伟, 等. 黄水生物转化技术研究[J]. 酿酒科技,2011(11):65−72.
[11]	郭憬, 付苗苗, 张慧, 等. 黄水生产酱油工艺研究[J]. 西北大学学报,2009(39):82−83.
[12]	王永伟, 谢杰, 张聪芝, 等. 黄水醋饮生产工艺研究[J]. 酿酒科技,2016(3):52−56.
[13]	杨慧敏, 曾礼兰, 贺富强, 等. 响应面法优化超压肠杆菌利用黄水生产微生物絮凝剂[J]. 中国酿造,2020,39(2):78−83. doi: 10.11882/j.issn.0254-5071.2020.02.014
[14]	李周, 贺富强, 杨惠敏, 等. 葡糖醋杆菌利用黄水发酵生产细菌纤维素[J]. 中国酿造,2018,37(1):54−58. doi: 10.11882/j.issn.0254-5071.2018.01.012
[15]	梁金钟, 毕明月, 王风青, 等. γ-聚谷氨酸在冰激凌中的应用研究[J]. 农产品加工,2016(23):6−10, 13.
[16]	宋玉萍, 梁金钟, 王风青, 等. 高分子聚合物γ-PGA对长双歧杆菌的冻干保护作用[J]. 食品工业科技,2016,37(9):185−189.
[17]	疏秀林, 施庆珊, 冯劲, 等. γ-聚谷氨酸对荔枝常温货架保鲜效果研究[J]. 食品工业科技,2012,33(21):318−321, 325.
[18]	张二伟, 刘宁, 吴涛, 等. γ-PGA的基本特性、生产方法及相关应用[J]. 食品工业科技,2018,39(16):318−324.
[19]	Fengqing Wang, Jinzhong Liang, et al. Improved production of poly-γ-glutamate by newly Bacillus subtilis Bs-115[J]. Journal of Biobased Materials and Bioenergy,2017(11):159−168.
[20]	王卫. γ-聚谷氨酸高产菌株的选育及培养基的优化[D]. 郑州: 河南工业大学, 2016.
[21]	梁金钟, 王风青, 张露露. 产γ-PGA批式发酵动力学模型的建立[J]. 食品工业科技,2013,34(16):174−177.
[22]	梁金钟, 王风青, 曲敏, 等. 微生物发酵法玉米生产高分子聚合物γ-PGA的中试研究[R]. 哈尔滨: 哈尔滨商业大学, 2012-05-31.
[23]	徐亚超, 盛杰, 刘安军. 白酒发酵副产物黄水抑菌特性及稳定性研究[J]. 食品科学,2017,38(15):122−126. doi: 10.7506/spkx1002-6630-201715020
[24]	盛杰, 纪海玉, 徐亚超, 等. 酿酒黄水对枯草芽孢杆菌的抑菌机制研究[J]. 食品工业科技,2017,38(21):126−129, 136.
[25]	彭英云. γ-聚谷氨酸生产、合成机制和抗冷冻性的研究[D]. 无锡: 江南大学, 2015.
[26]	Zhao Z Y, Ma S S, Li A, et al. Effects of trophic modes, carbon sources, and salinity on the cell growth and lipid accumulation of tropic ocean oilgae strain Desmodesmus sp. WC08[J]. Applied Biochemistry and Biotechnology,2016,180(3):452−463. doi: 10.1007/s12010-016-2109-5
[27]	Zhang H, Zhu J, Zhu X, et al. High-level exogenous glutamic acid-independent production of poly-(γ-glutamic acid) with organic acid addition in a new isolated Bacillus subtilis C10[J]. Bioresource Technology,2012,116:241−246. doi: 10.1016/j.biortech.2011.11.085
[28]	Huang B, Qin P, Xu Z, et al. Effects of CaCl₂ on viscosity of culture broth, and on activities of enzymes around the 2-oxoglutarate branch, in Bacillus subtilis CGMCC 2108 producing poly-(γ-glutamic acid)[J]. Bioresource Technology,2011,102(3):3595−3598. doi: 10.1016/j.biortech.2010.10.073
[29]	Bi Fangcheng, Ment Dana, Luria Neta, et al. Mutation of AREA affects growth, sporulation, nitrogen regulation, and pathogenicity in Colletotrichum gloeosporioides[J]. Fungal Genetics and Biology: FG & B,2017,99:29−39.
[30]	刘青芝. 微生发酵法生产γ-聚谷氨酸的研究[D]. 济南: 山东大学, 2010.
[31]	张兰威. 发酵食品原理与技术[M]. 北京: 科学出版社, 2018, 41−45.
[32]	Kimura K, Tran LS, Uchida I, et al. Characterization of Bacillus subtilis gamma-glutamyltransferase and its involvement in the degradation of capsule poly-gamma-glutamate[J]. Microbiology,2004,150:4115−4123. doi: 10.1099/mic.0.27467-0
[33]	Richard A, Margaritis A. Kinetics of molecular weight reduction of poly(glutamic acid) by in situ depolymerization in cell-free broth of Bacillus subtilis[J]. Biochem Eng J,2006,30:303−307. doi: 10.1016/j.bej.2006.05.014
[34]	Yao J, Jing J, Xu H, et al. Investigation on enzymatic degradation of g-polyglutamic acid from Bacillus subtilis NX-2[J]. J Mol Catal B Enzym,2009,56:158−164. doi: 10.1016/j.molcatb.2007.12.027
[35]	Urushibata Y, Tokuyama S, Tahara Y. γ-Polyglutamic acid productivity of Bacillus subtilis NR-1 mutant defective of γ-glutamyltranspeptidase gene[R]. 日本生物工学会大会講演要旨集, Japan: The Society for Bioscience and Bioengineering, 1997: 115.
[36]	王风青, 梁金钟, 付大伟, 等. γ-PGA降解酶系基因pgdS和ggt的克隆及生物信息分析[J]. 食品工业科技,2016(16):190−194.