Determination of Lycopence in Tomato Products by High Performance Liquid Chromatography and the Correlation with Chromaticity Values

YANG Xuefan; ZHANG Wei; QIU kai; WANG Zhengwu

doi:10.13386/j.issn1002-0306.2020070373

Volume 42 Issue 13

Jun. 2021

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Science and Technology of Food Industry > 2021 > 42(13): 284-291. > DOI: 10.13386/j.issn1002-0306.2020070373

YANG Xuefan, ZHANG Wei, QIU kai, et al. Determination of Lycopence in Tomato Products by High Performance Liquid Chromatography and the Correlation with Chromaticity Values[J]. Science and Technology of Food Industry, 2021, 42(13): 284−291. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020070373.

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Determination of Lycopence in Tomato Products by High Performance Liquid Chromatography and the Correlation with Chromaticity Values

1.
School of Food Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China
2.
China National Research Institute of Food & Fermentation Industries Co., Ltd, Beijing 100020, China

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Received Date: July 28, 2020
Available Online: June 03, 2021

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Abstract

Abstract

Anvalidated analytical method based on High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD) was established for the determination of lycopene and its isomers in ketchup. The correlation between the color variables and lycopene content of tomato products from different manufacturers was also discussed. Lycopene was separated by using YMC-carotenoid (C₃₀, 250 mm×4.6 mm, 5 μm) column. Mobile phase contained methanol (A) and methyl tert-butyl ether (B) with flow rate of 1.3 mL/min and was monitored at 472 nm by using DAD. Content of lycopene had a good linear relationship in the concentration range of 10~200 μg/mL (R²=0.9997). The average recoveries ranged from 98.3%~99.6%.The accuracy, expressed in terms of relative standard deviation (RSD), ranged from 2.8% to 4.5%. The method was simple, sensitive, accurate and suitable for the detection of total lycopene in ketchup. The distribution of (all-E)-lycopene and Z-isomers in tomato products was detected and analyzed. The correlation analysis indicated that a*, chroma, a*/b*, hue had remarkable or extremely remarkable correlation with the content of lycopene, the optimal fitting result of regression was R²=0.689.
- high performance liquid chromatography (HPLC),
- lycopene,
- tomato products,
- chromaticity values,
- correlation analysis

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References (31)

References

[1]	Sigurdson G T, Tang P, Giusti M Mónica. Natural colorants: Food colorants from natural sources[J]. Annual Review of Food Science & Technology,2017,8(1):261−280.
[2]	Arias R, Lee T C, Logendra L, et al. Correlation of lycopene measured by HPLC with the L, a b* color readings of a hydroponic tomato and the relationship of maturity with color and lycopene content[J]. Journal of Agricultural and Food Chemistry,2000,48(5):1697−1702. doi: 10.1021/jf990974e
[3]	Costa-Rodrigues J, Pinho O, Monteiro P R R. Can lycopene be considered an effective protection against cardiovascular disease?[J]. Food Chemistry,2018,245(15):1148−1153.
[4]	Cristina Trejo-Solís, Jose Pedraza-Chaverrí, Mónica Torres-Ramos, et al. Multiple molecular and cellular mechanisms of action of lycopene in cancer inhibition[J]. Evidence-Based Complementray and Alternative Medicine,2013,1(10):705121−705138.
[5]	Ono M, Takeshima M, Nakano S. Mechanism of the anticancer effect of lycopene (tetraterpenoids)[J]. The Enzymes,2015,37(6):139−166.
[6]	王涵, 伍志超. 番茄红素对脓毒症大鼠免疫及炎性因子的影响[J]. 中国药业,2020,29(11):26−29. doi: 10.3969/j.issn.1006-4931.2020.11.007
[7]	Hussain A, Pu H, Sun D W J C R I F S, et al. Measurements of lycopene contents in fruit: A review of recent developments in conventional and novel techniques[J]. Critical Reviews in Food Science & Nutrition,2018,1(6):1−12.
[8]	Liu T, Zhu W, Huang J, et al. Comparison of the nutritional as well as the volatile composition of in-season and off-season Hezuo 903 tomato at red stage[J]. European Food Research and Technology,2017,243(2):203−214. doi: 10.1007/s00217-016-2736-7
[9]	李岩, 王丽伟, 文莲莲, 等. 红蓝光质对转色期间番茄果实主要品质的影响[J]. 园艺学报,2017,44(12):2372−2382.
[10]	Honda M, Kageyama H, Hibino T, et al. Enhanced Z-isomerization of tomato lycopene through the optimal combination of food ingredients[J]. Scientific Reports,2019,7979(9):1−7.
[11]	Honda M, Kudo T, Kuwa T, et al. Isolation and spectral characterization of thermally generated multi-Z-isomers of lycopene and the theoretically preferred pathway to di-Z-isomers[J]. Bioscience Biotechnology and Biochemistry,2017,81(2):365−371.
[12]	陈冬东, 郗存显, 彭涛, 等. 高效液相色谱-串联质谱法测定保健食品中的番茄红素[J]. 食品科学,2012,33(20):206−211.
[13]	Shahzad T, Ahmad I, Choudhry S, et al. DPPH free radical scavenging activity of tomato, cherry tomato and watermelon: Lycopene extraction, purification and quantification[J]. International Journal of Pharmacy & Pharmaceutical Sciences,2014,6(2):223−228.
[14]	于颖, 张维, 谢凡, 等. 改善番茄红素生物利用度的研究进展[J]. 食品科学,2019,40(19):346−352. doi: 10.7506/spkx1002-6630-20181011-094
[15]	Murakami K, Honda M, Wahyudiono, et al. Thermal isomerization of (all-E)-lycopene and separation of the Z-isomers by using a low boiling solvent: Dimethyl ether[J]. Separation Science and Technology,2017,52(16):2573−2582. doi: 10.1080/01496395.2017.1374412
[16]	国艳梅, 杜永臣, 王孝宣, 等. 利用色差仪估测番茄果实番茄红素含量的研究[J]. 中国蔬菜,2008,1(11):10−14.
[17]	Bicanic D, Dimitrovski D, Luterotti S, et al. Correlation of trans-lycopene measurements by the HPLC method with the optothermal and photoacoustic signals and the color readings of fresh tomato homogenates[J]. Food Biophysics,2010,5(1):24−33. doi: 10.1007/s11483-009-9140-9
[18]	He L, Jian Z, Yong W, et al. The Effects of storage conditions on lycopene content and color of tomato hot pot sauce[J]. International Journal of Analytical Chemistry,2018,1(1):1−8.
[19]	Fu X, He X, Xu H, et al. Nondestructive and rapid assessment of intact tomato freshness and lycopene content based on a miniaturized raman spectroscopic system and colorimetry[J]. Food Analytical Methods,2016,9(9):2501−2508. doi: 10.1007/s12161-016-0440-7
[20]	赵文启. 冷热破工艺对番茄酱贮藏期间品质变化影响的研究[D]. 石河子: 石河子大学, 2018.
[21]	牟琴, 徐俐, 鲁青松, 等. 不同发酵方式对发酵番茄酱品质的影响[J]. 食品工业,2019,40(6):50−54.
[22]	Kateřina Osterrothová, Adam Culka, Kateřina Němečková, et al. Analyzing carotenoids of snow algae by Raman microspectroscopy and high-performance liquid chromatography[J]. Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy,2019,4(212):262−271.
[23]	Withnall R, Chowdhry B Z, Silver J, et al. Raman spectra of carotenoids in natural products[J]. Spectrochimica Acta Part a-Molecular and Biomolecular Spectroscopy,2003,59(10):2207−2212. doi: 10.1016/S1386-1425(03)00064-7
[24]	Bao Y, Liu F, Kong W, et al. Measurement of soluble solid contents and ph of white vinegars using VIS/NIR spectroscopy and least squares support vector machine[J]. Food and Bioprocess Technology,2014,7(1):54−61.
[25]	Ding X, Guo Y, Ni Y, et al. A novel NIR spectroscopic method for rapid analyses of lycopene, total acid, sugar, phenols and antioxidant activity in dehydrated tomato samples[J]. Vibrational Spectroscopy,2016,82(1):1−9.
[26]	Tilahun S, Park D S, Seo M H, et al. Prediction of lycopene and β-carotene in tomatoes by portable chroma-meter and VIS/NIR spectra[J]. Postharvest Biology and Technology,2018,136:50−56. doi: 10.1016/j.postharvbio.2017.10.007
[27]	Herrero A M. Raman spectroscopy a promising technique for quality assessment of meat and fish: A review[J]. Food Chemistry,2008,107(4):1642−1651. doi: 10.1016/j.foodchem.2007.10.014
[28]	Agnieszka Nawrocka, Monika Szymańska-Chargot, Antoni Miś, et al. Raman studies of gluten proteins aggregation induced by dietary fibres[J].Food Chemistry, 2016, 194(1): 86−94.
[29]	惠伯棣, 张西, 文镜. 反相C_(30)柱在HPLC分析类胡萝卜素中的应用[J]. 食品科学,2005(1):264−270. doi: 10.3321/j.issn:1002-6630.2005.01.062
[30]	Saeid A, Eun J B, Sagor M S A, et al. Effects of extraction and purification methods on degradation kinetics and stability of lycopene from watermelon under storage conditions[J]. Journal of Food Science,2016,81(11):2630−2638. doi: 10.1111/1750-3841.13504
[31]	Gregory A Chasse, Melody L Mak, Eugen Deretey, et al. An ab initio computational study on selected lycopene isomers[J]. Journal of Molecular Structure Theochem,2015,571(1−3):27−37.

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