Purification and Antioxidant Properties of Anthocyanins from Jingle Lycium ruthenicum

Objective: To investigate the purification technology and antioxidation ability of Lycium ruthenicum anthocyanins from Jingle. Methods: Based on the adsorption properties of selected five macroporous resins (HPD100、D101、NKA、AB-8、HPD400) for Lycium ruthenicum anthocyanins from Jingle, the optimum purification resins were screened and the purification parameters were optimized. The antioxidant activity of crude and purified Lycium ruthenicum anthocyanins from Jingle were investigated by DPPH, hydroxyl and ABTS free radical scavenging assay. Results: HPD100 resin had good separation and purification effect. The optimum purification parameters were as follows: Sample concentration 0.2 mg/mL, sample volume 49 mL, elution volume 42 mL with 75% ethanol. After purification, the anthocyanins content was from 2.38% to17.82%. The crude and purified Lycium ruthenicum anthocyanins from Jingle showed good antioxidant activity, the IC₅₀ values of DPPH· scavenging capacity were 0.208 and 0.011 mg/mL respectively, the IC₅₀ values of ABTS⁺· scavenging capacity were 0.476 and 0.064 mg/mL respectively, the IC₅₀ value of purified Lycium ruthenicum anthocyanins for ·OH scavenging capacity was 6.24 mg/mL. Conclusion: The macroporous resin method for purifying Lycium ruthenicum anthocyanins has good purification effect. The antioxidant capacity of the purified Lycium ruthenicum anthocyanins was significantly improved compared with the crude extracts.