Analysis of Different Proteins Affecting Water Holding Capacity of Pork Based on Microstructure and Proteomics

Boruo YANG; Huajian LI; Yaning SU; Xia LI; Jing QU; Tao CHEN

doi:10.13386/j.issn1002-0306.2020070148

Volume 42 Issue 7

Mar. 2021

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Science and Technology of Food Industry > 2021 > 42(7): 136-144. > DOI: 10.13386/j.issn1002-0306.2020070148

YANG Boruo, LI Huajian, SU Yaning, et al. Analysis of Different Proteins Affecting Water Holding Capacity of Pork Based on Microstructure and Proteomics[J]. Science and Technology of Food Industry, 2021, 42(7): 136−144. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020070148.

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Analysis of Different Proteins Affecting Water Holding Capacity of Pork Based on Microstructure and Proteomics

College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China

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Received Date: July 13, 2020
Available Online: January 27, 2021

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Abstract

Abstract

In order to investigate the effects of microstructural and proteomic changes of fresh pork on their water holding capacity in the early postmortem period, the longissimus dorsi samples of pigs were divided into high drip loss group (H-group≥5.93%, n=3) and low drip loss group (L-group≤0.81%, n=3) according to the level of drip loss. The microstructure and proteomics of the two groups were compared. The difference proteins between the two groups were identified using Tandem Mass Tag (TMT) and intercellular spaces were observed using electron microscopy. The results showed that the extracellular space of H-group was significantly larger than that of L-group at 24 h after slaughter(P<0.01). The higher the expression level of glucose-phosphotransferase-1, heat shock protein 70 (Hsp70), ankyrin, selenoprotein W and laminin in postmortem muscle, the lower the drip loss and the better the water holding capacity, while the higher the expression level of phosphoglycerate mutase and transketolase, the higher the drip loss and the worse the water holding capacity.
- pork,
- proteomics,
- drip loss,
- biological metabolism,
- transketolase

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References (38)

References

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