Colistin-functionalized Fe3O4-GQDs as a Fluorescent Probe for Rapid Detection of Escherichia coli

XU Yangxin; GAO Xue; SUN Minjun; LIU Xiuying; LI Jianrong

doi:10.13386/j.issn1002-0306.2020070027

Volume 42 Issue 11

May 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(11): 9-14. > DOI: 10.13386/j.issn1002-0306.2020070027

XU Yangxin, GAO Xue, SUN Minjun, et al. Colistin-functionalized Fe₃O₄-GQDs as a Fluorescent Probe for Rapid Detection of Escherichia coli [J]. Science and Technology of Food Industry, 2021, 42(11): 9−14. (in Chinese with English abstract). doi: 10.13386/ j.issn1002-0306.2020070027.

Citation:

PDF (1162 KB)

Colistin-functionalized Fe₃O₄-GQDs as a Fluorescent Probe for Rapid Detection of Escherichia coli

School of Food Science and Engineering, Bohai University, Food Safety Key Lab of Liaoning Province, National and Local Joint Engineering Research Center for Storage and Processing of Fresh Agricultural Products and Safety Control Technology, Jinzhou 121013, China

More Information

Received Date: July 02, 2020
Available Online: March 29, 2021

Graphical Abstract

Abstract

Abstract

Objective: To establish a method for the determination of Gram-negative bacteria - Escherichia coli by Colis-Fe₃O₄-graphene quantum dots (GQDs) functionalized fluorescent probes. Methods: Amino modified Fe₃O₄ was prepared by co-precipitation method, and it was combined with GQDs containing carboxyl groups, and Colis-Fe₃O₄-GQDs complex was formed by colistin modification. With this complex as fluorescent probe, different concentrations of Escherichia coli were detected. Results: The fluorescence quenching effect of Colis-Fe₃O₄-GQDs complex was good with Escherichia coli, and the fluorescence quenching of Colis-Fe₃O₄-GQDs complex was gradually enhanced with the increasing concentration of the strain. The linear detection range of this method was 1×10²~10×10² CFU/mL, the coefficient of determination was 0.999, and the detection limit was 0.36×10² CFU/mL. Conclusion: The colis-Fe₃O₄-GQDs biosensor is simple and efficient, with good selectivity and sensitivity, which provides a new method for the detection of Gram-negative bacteria, and has a broad application prospect in the detection of bacteria.
- Fe₃O₄-GQDs,
- colistin,
- Gram-negative bacteria,
- fluorescent probe

FullText(HTML)

References (45)

References

[1]	Wang Z F, Liu F. Nanopatterned graphene quantum dots as building blocks for quantum cellular automata[J]. Nanoscale,2011,3(10):4201−4205. doi: 10.1039/c1nr10489f
[2]	Li Y, Hu Y, Zhao Y, et al. An electrochemical avenue to green-luminescent graphene quantum dots as potential electron-acceptors for photovoltaics[J]. Advanced materials,2011,23(6):776−780. doi: 10.1002/adma.201003819
[3]	Yan X, Cui X, Li L. Synthesis of large, stable colloidal graphene quantum dots with tunable size[J]. Journal of the American Chemical Society,2010,132(17):5944−5945. doi: 10.1021/ja1009376
[4]	Feng Y, Zhong D, Miao H, et al. Carbon dots derived from rose flowers for tetracycline sensing[J]. Talanta,2015,140:128−133. doi: 10.1016/j.talanta.2015.03.038
[5]	Pan D, Zhang J, Li Z, et al. Hydrothermal route for cutting graphene sheets into blue-luminescent graphene quantum dots[J]. Advanced materials,2010,22(6):734−738. doi: 10.1002/adma.200902825
[6]	Liu R, Wu D, Feng X, et al. Bottom-up fabrication of photoluminescent graphene quantum dots with uniform morphology[J]. Journal of the American Chemical Society,2011,133(39):15221−15223. doi: 10.1021/ja204953k
[7]	Konstantatos G, Badioli M, Gaudreau L, et al. Hybrid graphene-quantum dot phototransistors with ultrahigh gain[J]. Nature Nanotechnology,2012,7(6):363. doi: 10.1038/nnano.2012.60
[8]	Xu C, Yang S, Tian L, et al. Fabrication of centimeter-scale light-emitting diode with improved performance based on graphene quantum dots[J]. Applied Physics Express,2017,10(3):032102. doi: 10.7567/APEX.10.032102
[9]	Wu X, Tian F, Wang W, et al. Fabrication of highly fluorescent graphene quantum dots using L-glutamic acid for in vitro/in vivo imaging and sensing[J]. Journal of Materials Chemistry C,2013,1(31):4676−4684. doi: 10.1039/c3tc30820k
[10]	李想, 李萍, 韩奎文. 革兰氏阴性杆菌的分布特点及耐药性分析[J]. 中国实验诊断学,2019(12):2121−2123. doi: 10.3969/j.issn.1007-4287.2019.12.036
[11]	Chua C K, Sofer Z, Simek P, et al. Synthesis of strongly fluorescent graphene quantum dots by cage-opening buckminsterfullerene[J]. Acs Nano,2015,9(3):2548−2555. doi: 10.1021/nn505639q
[12]	刘振宇, 刘瑾. 石墨烯量子点制备技术及创新资源现状[J]. 中国科技信息,2018(1):59−61. doi: 10.3969/j.issn.1001-8972.2018.01.022
[13]	Chen H, Xie Y, Kirillov A M, et al. A ratiometric fluorescent nanoprobe based on terbium functionalized carbon dots for highly sensitive detection of an anthrax biomarker[J]. Chemical Communications,2015,51(24):5036−5039. doi: 10.1039/C5CC00757G
[14]	Zhang Z, Zhang J, Chen N, et al. Graphene quantum dots: an emerging material for energy-related applications and beyond[J]. Energy & Environmental Science,2012,5(10):8869−8890.
[15]	曲丹. 掺杂型石墨烯量子点的制备及其应用研究[D]. 长春: 中国科学院长春光学精密机械与物理研究所, 2017.
[16]	Ding H, Yu S B, Wei J S, et al. Full-color light-emitting carbon dots with a surface-state-controlled luminescence mechanism[J]. ACS nano,2015,10(1):484−491.
[17]	Zhang M, Ju H, Zhang L, et al. Engineering iodine-doped carbon dots as dual-modal probes for fluorescence and X-ray CT imaging[J]. International Journal of Nanomedicine,2015,10:6943.
[18]	Hu S, Tian R, Dong Y, et al. Modulation and effects of surface groups on photoluminescence and photocatalytic activity of carbon dots[J]. Nanoscale,2013,5(23):11665−11671. doi: 10.1039/c3nr03893a
[19]	Yin J Y, Liu H J, Jiang S, et al. Hyperbranched polymer functionalized carbon dots with multistimuli-responsive property[J]. ACS Macro Letters,2013,2(11):1033−1037. doi: 10.1021/mz400474v
[20]	Robinson T, Mc Mullan G, Marchant R, et al. Remediation of dyes in textile effluent: A critical review on current treatment technologies with a proposed alternative[J]. Bioresource Technology,2001,77(3):247−255. doi: 10.1016/S0960-8524(00)00080-8
[21]	Labanda J, Sabaté J, Llorens J. Modeling of the dynamic adsorption of an anionic dye through ion-exchange membrane adsorber[J]. Journal of Membrane Science,2009,340(1−2):234−240. doi: 10.1016/j.memsci.2009.05.036
[22]	Wu J S, Liu C H, Chu K H, et al. Removal of cationic dye methyl violet 2B from water by cation exchange membranes[J]. Journal of Membrane Science,2008,309(1-2):239−245. doi: 10.1016/j.memsci.2007.10.035
[23]	Wesenberg D, Kyriakides I, Agathos S N. White-rot fungi and their enzymes for the treatment of industrial dye effluents[J]. Biotechnology Advances,2003,22(1-2):161−187. doi: 10.1016/j.biotechadv.2003.08.011
[24]	Paszczynski A, Crawford R L. Degradation of azo compounds by ligninase from Phanerochaete chrysosporium: Involvement of veratryl alcohol[J]. Biochemical and Biophysical Research Communications,1991,178(3):1056−1063. doi: 10.1016/0006-291X(91)90999-N
[25]	陈冬梅, 陈求刚, 廖康, 等. 院内常见革兰氏阴性杆菌耐药性监测[J]. 中国抗生素杂志,2001,26(6):473−476. doi: 10.3969/j.issn.1001-8689.2001.06.020
[26]	Johnson J R, Johnston B, Clabots C, et al. Escherichia coli sequence type ST131 as the major cause of serious multidrug-resistant E. coli infections in the United States[J]. Clinical Infectious Diseases,2010,51(3):286−294. doi: 10.1086/653932
[27]	R Eisler E, Eisenberg H, Minton A P. Temperature and density dependence of the refractive index of pure liquids[J]. Journal of the Chemical Society, Faraday Transactions 2: Molecular and Chemical Physics,1972,68:1001. doi: 10.1039/f29726801001
[28]	马序竹, 单爱莲, 陈旭岩, 等. 多黏菌素的临床药学研究进展[J]. 中国临床药理学杂志,2017,33(7):665−667, 672.
[29]	赵苗, 张菁, 张婴元, 等. 多黏菌素临床研究进展[J]. 中国感染与化疗杂志,2017,17(6):695−702.
[30]	左联. 革兰氏阴性细菌的耐药机理[J]. 国际药学研究杂志,1999(2):101−104.
[31]	Falagas M E, Bliziotis I A. Pandrug-resistant Gram-negative bacteria: The dawn of the post-antibiotic era?[J]. International Journal of Antimicrobial Agents,2007,29(6):630−636. doi: 10.1016/j.ijantimicag.2006.12.012
[32]	Johnston M. Escherichia coli—a review[J]. Journal of the Royal Society for the Promotion of Health,2000,120(2):81.
[33]	Otieno B A, Krause C E, Rusling J F. Bioconjugation of antibodies and enzyme labels onto magnetic beads[J]. Methods in Enzymology,2016,571:135.
[34]	Liu X, Ma Z, Xing J, et al. Preparation and characterization of amino-silane modified superparamagnetic silca nanospheres[J]. Journal of Magnetism and Magnetic Materials,2004,270(1):1.
[35]	孙宁, 胡飞. 超顺磁性颗粒表面复合修饰及用于固定化α-11淀粉酶载体的效果[J]. 农业工程学报,2016,32(11):290−294. doi: 10.11975/j.issn.1002-6819.2016.11.041
[36]	贺全国, 吴伟, 林琳. 表面氨基化磁性Fe₃O₄纳米粒子合成与表征[J]. 自然科学版,2007,21(1):19−24, 29.
[37]	倪丹妮, 蒋栋能, 蒲晓允. 基于石墨烯量子点和Fe₃O₄纳米复合探针的肾病管型的荧光成像技术的研究[J]. 检验医学与临床,2016,13(17):2414−2416. doi: 10.3969/j.issn.1672-9455.2016.17.003
[38]	刘斌. Pickering乳液在药物胶囊中的应用[D]. 北京: 北京化工大学, 2017.
[39]	宋宇. 基于石墨烯量子点仿生纳米材料对鱼肉中喹诺酮类药物的应用研究[D]. 锦州: 渤海大学, 2020.
[40]	洪勇. Fe₃O₄基纳米复合材料的制备和磁光性能研究[D]. 合肥: 合肥工业大学, 2018.
[41]	周磊. 秦皮素对大肠杆菌的抑菌作用机制[D]. 大连: 辽宁师范大学, 2013.
[42]	顾银君, 王秀玲, 陈恭, 等. 氨基硅烷化磁性纳米粒子的制备与表征[J]. 苏州科技学院学报: 自然科学版,2012,29(1):42−46.
[43]	刘微波. Fe₃O₄磁性纳米粒子的制备、表征及其在分离检测中的应用[D]. 无锡: 江南大学, 2010.
[44]	崔明通. 氨基改性纳米Fe₃O₄吸附剂的制备及其对水溶液中Cr(Ⅵ)和Pb(Ⅱ)的吸附[D]. 天津: 天津大学, 2016.
[45]	陈杖榴. 兽药药理学[M]. 北京: 中国农业出版社, 2002: 225−226.

Supplements (0)

Cited By

Cited by

Periodical cited type(8)

1.	王佳，丁方莉，安宇，曾雪莹，张智慧，李思楠，徐开媛，周芳，王颖，张璐，徐炳政，孙泽堃. 芸豆-蓝靛果复合发酵液制备工艺优化及其抗氧化活性. 食品工业科技. 2025(03): 222-231 . 本站查看
2.	王虎玄，柯西娜，王聪，朱亚南，孙宏民. 苹果酵素的制备及其抗氧化功能研究. 陕西科技大学学报. 2023(03): 37-46 .
3.	杨彬彦，党娅，黎坤怡. 蓝莓酵素复合菌种发酵工艺优化及品质分析. 中国酿造. 2023(12): 165-169 .
4.	陈洲琴，张祝兰，程贤，林仙菊，杨煌建，严雪浪，朱爱明，连云阳. 枇杷酵素发酵过程生物学特性和主要功效酶活性研究. 福建农业科技. 2023(10): 23-28 .
5.	秦宇蒙，王艳丽，周笑犁，董平坤，吴栋斐. 番茄酵素自然发酵过程中主要功效酶的变化. 食品工业科技. 2022(20): 60-66 . 本站查看
6.	蒋家璇，韩盼盼，孔振杨，程陆陆，姚沛琳. 百香果酵素自然发酵过程中代谢产物及抗氧化活性研究. 农产品加工. 2022(19): 10-13+17 .
7.	任秀秀，余冬丽，郭涛，郭正江，LUO Liu. 餐余酵素中益生菌的分离培养及鉴定. 贵州工程应用技术学院学报. 2021(03): 61-67 .
8.	张焱梅，甘玉芬，丁学梅，马海燕，伍凤莲，冯玉兰. 植物酵素的活性功效及其食用方面的研究进展. 甘肃科技纵横. 2021(08): 25-28 .