Quantitative Determination of Phosphate in Meat Products by <sup>31</sup>P-Nuclear Magnetic Resonance

Zhi HAN; Feng JIANG; Mi ZHOU; Yazhen ZHANG; Lei GONG; Huixia WANG

doi:10.13386/j.issn1002-0306.2020060360

Volume 42 Issue 9

Apr. 2021

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Science and Technology of Food Industry > 2021 > 42(9): 275-280. > DOI: 10.13386/j.issn1002-0306.2020060360

HAN Zhi, JIANG Feng, ZHOU Mi, et al. Quantitative Determination of Phosphate in Meat Products by ³¹P-Nuclear Magnetic Resonance[J]. Science and Technology of Food Industry, 2021, 42(9): 275−280. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306. 2020060360.

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Quantitative Determination of Phosphate in Meat Products by ³¹P-Nuclear Magnetic Resonance

Hubei Provincial Institute for Food Supervision and Test, Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan 430075, China

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Received Date: July 01, 2020
Available Online: March 15, 2021

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Abstract

Abstract

A ³¹P-quantitative nuclear magnetic resonance (³¹P-qNMR) technique was established for determination of content of various phosphates in meat products. The samples were extracted twice with alkaline buffer salts, after shaking and centrifugation through the membrane, the target compounds quantified analysis by NMR using hexamethyl phosphoramide as internal standard. The results showed that: The recovery rates of phosphates were 97.0%～112.0%, and the RSDs were 0.87%～3.15%, the intra-day and inter-day precisions were 0.68%～3.55%, 1.12%～4.51%, respectively. The phosphates in commercial meat products were detected, and the qNMR measurement results were accurate. This method simplified sample pretreatment, saved time, quantified accurately, and had wider applicability for quantitative detection of phosphates in foods. It provides a new method for the quantitative determination of phosphates in meat products.
- quantitative nuclear magnetic resonance (qNMR),
- ³¹P-nuclear magnetic resonance (³¹P-NMR),
- meat products,
- phosphates

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