HAN Zhi, JIANG Feng, ZHOU Mi, et al. Quantitative Determination of Phosphate in Meat Products by 31P-Nuclear Magnetic Resonance[J]. Science and Technology of Food Industry, 2021, 42(9): 275−280. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306. 2020060360.
Citation: HAN Zhi, JIANG Feng, ZHOU Mi, et al. Quantitative Determination of Phosphate in Meat Products by 31P-Nuclear Magnetic Resonance[J]. Science and Technology of Food Industry, 2021, 42(9): 275−280. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306. 2020060360.

Quantitative Determination of Phosphate in Meat Products by 31P-Nuclear Magnetic Resonance

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  • Received Date: July 01, 2020
  • Available Online: March 15, 2021
  • A 31P-quantitative nuclear magnetic resonance (31P-qNMR) technique was established for determination of content of various phosphates in meat products. The samples were extracted twice with alkaline buffer salts, after shaking and centrifugation through the membrane, the target compounds quantified analysis by NMR using hexamethyl phosphoramide as internal standard. The results showed that: The recovery rates of phosphates were 97.0%~112.0%, and the RSDs were 0.87%~3.15%, the intra-day and inter-day precisions were 0.68%~3.55%, 1.12%~4.51%, respectively. The phosphates in commercial meat products were detected, and the qNMR measurement results were accurate. This method simplified sample pretreatment, saved time, quantified accurately, and had wider applicability for quantitative detection of phosphates in foods. It provides a new method for the quantitative determination of phosphates in meat products.
  • [1]
    闫家荫, 刘瑞英, 康明丽. 复合磷酸盐在肉制品中的应用及研究进展[J]. 农产品加工,2020(2):82−84.
    [2]
    Jin H, Xu C X, Lim H T, et al. High dietary inorganic phosphate increases lung tumorigenesis and alters Akt signaling[J]. American journal of respiratory and critical care medicine,2009,179(1):59−68. doi: 10.1164/rccm.200802-306OC
    [3]
    国家卫生和计划生育委员会. GB 2760-2014食品安全国家标准食品添加剂使用标准[S]. 北京: 中国标准出版社, 2014.
    [4]
    刘佳, 沈娟, 詹玮, 等. 采用离子色谱检测肉及肉制品中多聚磷酸盐[J]. 食品与发酵工业,2017,43(9):215−218.
    [5]
    国家标准化管理委员会, 国家质量监督检验检疫总局. GB/T 9695.9-2009 肉与肉制品聚磷酸盐测定[S]. 中国标准出版社, 2009.
    [6]
    国家卫生和计划生育委员会, 国家食品药品监督管理总局. GB 5009.87-2016 食品安全国家标准食品中磷的测定[S]. 中国标准出版社, 2016.
    [7]
    国家质量监督检验检疫总局. SN/T 4590-2016出口水产品中焦磷酸盐、三聚磷酸盐、三偏磷酸盐含量的测定离子色谱法[S]. 中国标准出版社, 2017.
    [8]
    国家卫生和计划生育委员会. GB 5009.256-2016食品安全国家标准食品中多种磷酸盐的测定[S]. 中国标准出版社, 2016.
    [9]
    Gimbert L J, Haygarth P M, Worsfold P J. Determination of nanomolar concentrations of phosphate in natural waters using flow injection with a long path length liquid waveguide capillary cell and solid-state spectrophotometric detection[J]. Talanta,2007,71(4):1624−1628. doi: 10.1016/j.talanta.2006.07.044
    [10]
    Jastrzębska A, Hol A, Szłyk E. Simultaneous and rapid determination of added phosphorus (V) compounds in meat samples by capillary isotachophoresis[J]. LWT-Food Science and Technology,2008,41(10):2097−2103. doi: 10.1016/j.lwt.2007.11.015
    [11]
    Wijnker J J, Tjeerdsma-van Bokhoven J L M, Veldhuizen E J A. Phosphate analysis of natural sausage casings preserved in brines with phosphate additives as inactivating agent-Method validation[J]. Meat Science,2009,81(1):245−248. doi: 10.1016/j.meatsci.2008.07.027
    [12]
    周霞云. 分散液相微萃取-定量核磁共振 31P谱测定农田土壤中有机磷农药残留[D]. 南京: 南京农业大学, 2017.
    [13]
    罗敬. 利用核磁共振法分析几种含磷物质中磷的形态与含量[D]. 长沙: 湖南师范大学, 2015.
    [14]
    严伟. 果蔬中多农残19F和31P NMR定量检测方法的研究[D]. 北京: 北京化工大学, 2013.
    [15]
    Spyros A, Dais P. 31P NMR spectroscopy in food analysis[J]. Progress in Nuclear Magnetic Resonance Spectroscopy,2009,54(3-4):195−207. doi: 10.1016/j.pnmrs.2008.09.002
    [16]
    Rigger R, Rück A, Hellriegel C, et al. Certified reference material for use in 1H, 31P, and 19F quantitative NMR, ensuring traceability to the International System of Units[J]. Journal of AOAC International,2017,100(5):1365−1375. doi: 10.5740/jaoacint.17-0093
    [17]
    Stücker A, Podschun J, Saake B, et al. A novel quantitative 31P NMR spectroscopic analysis of hydroxyl groups in lignosulfonic acids[J]. Analytical Methods,2018,10(28):3481−3488. doi: 10.1039/C8AY01272E
    [18]
    周凝, 刘宝林, 王欣. 核磁共振技术在食品分析检测中的应用[J]. 食品工业科技,2011,32(1):325−329.
    [19]
    Sojka S A, Wolfe R A. Analysis of commercial sodium tripolyphosphates by phosphorus-31 Fourier transform nuclear magnetic resonance spectrometry[J]. Analytical Chemistry,1978,50(4):585−587. doi: 10.1021/ac50026a011
    [20]
    Stanislawski D A, Van Wazer J R. Determination of phosphorus compounds by phosphorus-31 Fourier transform nuclear magnetic resonance spectrometry[J]. Analytical Chemistry,1980,52(1):96−101. doi: 10.1021/ac50051a023
    [21]
    Hrynczyszyn P, Jastrzębska A, Szłyk E. Determination of phosphate compounds in meat products by 31-phosphorus nuclear magnetic resonance spectroscopy with methylenediphosphonic acid after alkaline extraction[J]. Analytica Chimica Acta,2010,673(1):73−78.
    [22]
    Szłyk E, Hrynczyszyn P. Phosphate additives determination in meat products by 31-phosphorus nuclear magnetic resonance using new internal reference standard: Hexamethylphosphoroamide[J]. Talanta,2011,84(1):199−203. doi: 10.1016/j.talanta.2010.12.046
    [23]
    吉鑫, 樊双喜, 杨彤晖, 等. 核磁共振法测定食品中水苏糖的含量[J]. 食品与发酵工业,2019,45(19):251−257.
    [24]
    肖坤, 龚灿, 郭强胜, 等. 定量核磁共振碳谱同时测定食用油中甘油三酯的sn-1, 3和sn-2脂肪酸含量[J]. 分析化学,2020,48(6):802−812.
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