Quantitative Determination of Phosphate in Meat Products by ³¹P-Nuclear Magnetic Resonance

A ³¹P-quantitative nuclear magnetic resonance (³¹P-qNMR) technique was established for determination of content of various phosphates in meat products. The samples were extracted twice with alkaline buffer salts, after shaking and centrifugation through the membrane, the target compounds quantified analysis by NMR using hexamethyl phosphoramide as internal standard. The results showed that: The recovery rates of phosphates were 97.0%～112.0%, and the RSDs were 0.87%～3.15%, the intra-day and inter-day precisions were 0.68%～3.55%, 1.12%～4.51%, respectively. The phosphates in commercial meat products were detected, and the qNMR measurement results were accurate. This method simplified sample pretreatment, saved time, quantified accurately, and had wider applicability for quantitative detection of phosphates in foods. It provides a new method for the quantitative determination of phosphates in meat products.