Effects of Hemp Seed Oil and Cannabidiol on Behavior and Inflammation in Depression Model Mice

Yucong WANG; Zhixin XIE; Chunyu LI; Yuqing WANG; Xiaowei CHEN; Jianchun HAN

doi:10.13386/j.issn1002-0306.2020060238

Volume 42 Issue 9

Apr. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(9): 327-333. > DOI: 10.13386/j.issn1002-0306.2020060238

WANG Yucong, XIE Zhixin, LI Chunyu, et al. Effects of Hemp Seed Oil and Cannabidiol on Behavior and Inflammation in Depression Model Mice[J]. Science and Technology of Food Industry, 2021, 42(9): 327−333. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020060238.

Citation:

PDF (1185 KB)

Effects of Hemp Seed Oil and Cannabidiol on Behavior and Inflammation in Depression Model Mice

1.
College of Food Science, Northeast Agricultural University, Harbin 150030, China
2.
Heilongjiang Green Food Science Research Institute, Harbin 150028, China

More Information

Received Date: June 21, 2020
Available Online: March 15, 2021

Graphical Abstract

Abstract

Abstract

Objective: To explore the effects of hemp seed oil and Cannabidiol (CBD) on the behavior and inflammatory factors of chronic unpredictable mild stress (CUMS) mice. Methods: 50 male C57BL/6 mice were randomly divided into 5 groups: Blank group, model group, hemp seed oil group (3 mL/kg), CBD low-dose (15 mg/kg), CBD high-dose group (30 mg/kg), and gavage was continued for 8 weeks. The expression of depression, anxiety, learning and cognitive ability and inflammatory factors (tumor necrosis factor (TNF-α), interleukin-1 (IL-1β) and induced NOS (iNos)) were measured in mice. Results: Compared with the blank group, the model group had significant differences in depression and anxiety behavior and learning and cognitive functions (P<0.05; P<0.01); compared with the model group, the CBD dose group significantly improved the model mice from the depressive and anxious behavior of mice to the cognitive function of learning (P<0.05; P<0.01); besides, the hemp seed oil group significantly improved the sucrose preference index and new object recognition ability in mice (P<0.05). Compared with the blank group, the expression of TNF-α, IL-1β and iNos mRNA in the cortex and hippocampus of the model group significantly increased (P<0.05; P<0.01); compared with the model group, CBD dose groups showed that the expression of TNF-α, IL-1β and iNos mRNA in the cortex and hippocampus significantly decreased (P<0.05; P<0.01); There was no significant difference in TNF-α and iNos in cortex and hippocampus of the hemp seed oil group(P>0.05). The results of immunohistochemistry showed that hemp seed oil and CBD both inhibited the expression of ionized calcium-binding adaptor molecule 1 (IBA-1) of inflammatory mediator microglia. Conclusion: CUMS mice are successfully modeled. Hemp oil can partially improve the depressive behavior and cognitive ability of mice and reduce the expression of IL-1β. The CBD can improve the anxiety symptoms of mice, increase learning and cognitive functions, and inhibit the cortex and hippocampus of brain tissue. The inflammatory response of hemp seed oil and CBD has a certain effect on CUMS model mice.
- mice,
- hemp seed oil,
- cannabidiol,
- depression,
- inflammatory factors

FullText(HTML)

References (37)

References

[1]	何锦风, 张琨, 陈天鹏. 汉麻籽的营养成分和功能[J]. 食品科技,2007(6):257−260. doi: 10.3969/j.issn.1005-9989.2007.06.077
[2]	张明发, 沈雅琴. 火麻仁药理研究进展[J]. 上海医药,2008,29(11):511−513. doi: 10.3969/j.issn.1006-1533.2008.11.010
[3]	萧闵, 李全胜. 火麻仁油与藻油混合物对营养肥胖大鼠的降脂减肥作用研究[J]. 湖北中医药大学学报,2016,18(4):12−15. doi: 10.3969/j.issn.1008-987x.2016.04.03
[4]	任汉阳, 张瑜, 刘红雨, 等.火麻仁油对便秘模型小鼠抗氧化作用的实验研究[J]. 中华中医药杂志, 2004, 19(2): 123−124.
[5]	苏婧, 贺海波, 石孟琼, 等.火麻仁油对D-半乳糖致衰老小鼠学习记忆障碍的保护作用研究[J]. 中国临床药理学与治疗学, 2011, 16(12): 1332−1339.
[6]	Citti C, Pacchetti B, Vandelli M A, et al. Analysis of cannabinoids in commercial hemp seed oil and decarboxylation kinetics studies of cannabidiolic acid (CBDA)[J]. Journal of Pharmaceutical and Biomedical Analysis,2018,149:532−540. doi: 10.1016/j.jpba.2017.11.044
[7]	Callaway J C. Hempseed as a nutritional resource: An overview[J]. Euphytica,2004,140(2):65−72.
[8]	李卫, 韩福军, 赵立涛, 等.大麻二酚在免疫系统中的研究进展[J]. 当代临床医刊, 2019, 32(3): 297−298.
[9]	Zuardi A W, Cosme R A, Graeff F G, et al. Effects of ipsapirone and cannabidiol on human experimental anxiety[J]. J Psychopharmacol,1993,7(1 Suppl):82−88.
[10]	Campos A C, Moreira F A, Gomes F V, et al. Multiple mechanisms involved in the large-spectrum therapeutic potential of cannabidiol in psychiatric disorders[J]. Philos Trans R Soc Lond B Biol Sci,2012,367(1607):3364−3378. doi: 10.1098/rstb.2011.0389
[11]	Iuvone T, Esposito G, Filippis D D, et al. Cannabidiol: A promising drug for neurodegenerative disorders?[J]. Cns Neuroscience,2009,15(1):65−75. doi: 10.1111/j.1755-5949.2008.00065.x
[12]	Lafuente H, Alvarez F J, Pazos M R, et al. Cannabidiol reduces brain damage and improves functional recovery after acute hypoxia-ischemia in newborn pigs[J]. Pediatric Research,2011,70(3):272−277. doi: 10.1203/PDR.0b013e3182276b11
[13]	Sartim A G, Guimarães F S, Joca S R L. Antidepressant-like effect of cannabidiol injection into the ventral medial prefrontal cortex—Possible involvement of 5-HT1A and CB1 receptors[J]. Behavioural Brain Research,2016,303:218−227. doi: 10.1016/j.bbr.2016.01.033
[14]	Sukoff Rizzo S J, Neal S J, Hughes Z A, et al. Evidence for sustained elevation of IL-6 in the CNS as a key contributor of depressive-like phenotypes[J]. J Translational Psychiatry, 2(12): e199.
[15]	牛雅杰. 紫锥菊提取物抗胰岛素抵抗作用及功能饮料的研制[D]. 杨凌: 西北农林科技大学, 2017.
[16]	Shoval G, Shbiro L, Hershkovitz L, et al. Prohedonic effect of cannabidiol in a rat model of depression[J]. Neuropsychobiology,2016,73(2):123−129. doi: 10.1159/000443890
[17]	李根林, 曹亚蕊, 吴宿慧, 等.火麻仁油对衰老模型小鼠血脂水平及炎症、抗氧化相关指标的影响[J]. 中药药理与临床, 2015(1): 109−111.
[18]	魏月媛, 李理. 火麻仁油安全性的毒理学评价[J]. 食品与机械,2016,32(3):4−11.
[19]	Liu Z, Chen Y, Qiao Q, et al. Sesamol supplementation prevents systemic inflammation-induced memory impairment and amyloidogenesis via inhibition of nuclear factor kappaB[J]. Mol Nutr Food Res,2017,61(5):1600734. doi: 10.1002/mnfr.201600734
[20]	Kalynchuk L E, Pinel J P J, Treit D, et al. Changes in emotional behavior produced by long-term amygdala kindling in rats[J]. Biological Psychiatry,1997,41(4):438−451. doi: 10.1016/S0006-3223(96)00067-4
[21]	Steru L, Chermat R, Thierry B, et al. The tail suspension test: A new method for screening antidepressants in mice[J]. Psychopharmacology (Berl),1985,85(3):367−370. doi: 10.1007/BF00428203
[22]	Mehta V, Parashar A, Udayabanu M. Quercetin prevents chronic unpredictable stress induced behavioral dysfunction in mice by alleviating hippocampal oxidative and inflammatory stress[J]. Physiol Behav,2017,171:69−78. doi: 10.1016/j.physbeh.2017.01.006
[23]	Arai K, Matsuki N, Ikegaya Y, et al. Deterioration of spatial learning performances in lipopolysaccharide-treated mice[J]. Jpn J Pharmacol,2001,87(3):195−201. doi: 10.1254/jjp.87.195
[24]	Ennaceur A, Delacour J. A new one-trial test for neurobiological studies of memory in rats. 1: Behavioral data[J]. Behavioural Brain Research,1988,31(1):47−59. doi: 10.1016/0166-4328(88)90157-X
[25]	桂才华. PCR技术在食品检测中的应用[J]. 科技创新导报,2018,437(5):120−122.
[26]	Ip F C F, Fu W-Y, Cheng E Y L, et al. Anemoside A3 enhances cognition through the regulation of synaptic function and neuroprotection[J]. Neuropsychopharmacology: Official publication of the American College of Neuropsychopharmacology,2015,40(8):1877−1887. doi: 10.1038/npp.2015.37
[27]	Holmes A, Rodgers R J. Responses of Swiss-Webster mice to repeated plus-maze experience: Further evidence for a qualitative shift in emotional state[J]. Pharmacology Biochemistry Behavior,1998,60(2):473−488. doi: 10.1016/S0091-3057(98)00008-2
[28]	Zanelati T V, Biojone C, Moreira F A, et al. Antidepressant-like effects of cannabidiol in mice: Possible involvement of 5-HT1A receptors[J]. Br J Pharmacol,2010,159(1):122−128. doi: 10.1111/j.1476-5381.2009.00521.x
[29]	Mcarthur R, Borsini F. Animal models of depression in drug discovery: A historical perspective[J]. Pharmacol Biochem Behav,2006,84(3):436−452. doi: 10.1016/j.pbb.2006.06.005
[30]	Li J, Huang S, Huang W, et al. Paeoniflorin ameliorates interferon-alpha-induced neuroinflammation and depressive-like behaviors in mice[J]. Oncotarget,2017,8(5):8264−8282. doi: 10.18632/oncotarget.14160
[31]	李萃萃, 张曼辉, 夏涵, 等. 大麻二酚抑制小鼠脊髓损伤后炎症反应及促进功能恢复的研究[J]. 河北医学,2017,23(5):767−770. doi: 10.3969/j.issn.1006-6233.2017.05.018
[32]	Kumar B, Kuhad A, Chopra K. Neuropsychopharmacological effect of sesamol in unpredictable chronic mild stress model of depression: Behavioral and biochemical evidences[J]. Psychopharmacology (Berl),2011,214(4):819−828. doi: 10.1007/s00213-010-2094-2
[33]	Li R, Zhao D, Qu R, et al. The effects of apigenin on lipopolysaccharide-induced depressive-like behavior in mice[J]. Neuroscience Letters,2015,594:17−22. doi: 10.1016/j.neulet.2015.03.040
[34]	Shelton R C, Claiborne J, Sidoryk-Wegrzynowicz M, et al. Altered expression of genes involved in inflammation and apoptosis in frontal cortex in major depression[J]. Molecular Psychiatry,2011,16(7):751−762. doi: 10.1038/mp.2010.52
[35]	Zhan Y, Paolicelli R C, Sforazzini F, et al. Deficient neuron-microglia signaling results in impaired functional brain connectivity and social behavior[J]. Nature Neuroscience,2014,17(3):400−406. doi: 10.1038/nn.3641
[36]	Basilico B, Pagani F, Grimaldi A, et al. Microglia shape presynaptic properties at developing glutamatergic synapses[J]. Glia,2019,67(1):53−67. doi: 10.1002/glia.23508
[37]	Kim Y K, Na K S, Myint A M, et al. The role of pro-inflammatory cytokines in neuroinflammation, neurogenesis and the neuroendocrine system in major depression[J]. Progress in Neuropsychopharmacology Biological Psychiatry,2016,64:277−284. doi: 10.1016/j.pnpbp.2015.06.008

Supplements (0)

Cited By

Cited by

Periodical cited type(9)

1.	袁求松，张玉，付湘晋，向延菊. 市售22种梅干菜滋味品质与理化指标相关性分析. 食品安全质量检测学报. 2025(06): 316-322 .
2.	刘仁杰，卞方圆，黄志远，伊奎鑫. 添加毛竹笋混合发酵对梅干菜主要功能性物质及营养成分变化的影响. 竹子学报. 2024(01): 11-18 .
3.	李珊慧，林登峰，赵洪雷，李学鹏，励建荣，徐永霞. 双响应值联合优化超声波辅助熬制雪菜大黄鱼汤工艺及其滋味评价. 中国调味品. 2024(06): 43-49+76 .
4.	李艺，童秀子，徐伟程，郭斯统，陈育如，郭宇星，孔晓雪，罗海波. 宁波地区传统梅干菜中优势发酵菌株分离鉴定及酶活分析. 食品工业科技. 2023(03): 154-162 . 本站查看
5.	史学敏，刘珊娜，杜茜. 鄂尔多斯地区酸粥细菌多样性分析及乳酸菌的分离鉴定. 食品工业科技. 2023(09): 153-159 . 本站查看
6.	任锡亮，高天一，莫旺成，王洁，孟秋峰. 不同盐度和腌制时间对雪菜的影响. 中国果菜. 2023(10): 25-28 .
7.	田丽君，荊永锋，郑卜凡，彭新辉，龚嘉蕾，吴涛，李正风，李振杰，田祥珅，周启运，龚嘉，黎娟. 浏阳洞库陈化烟叶微生物多样性分析. 中国烟草学报. 2022(01): 98-107 .
8.	庞春霞，李艺，虞任莹，甘甜，郭斯统，陈育如，罗海波. 基于Illumina Miseq技术比较不同地区传统发酵大豆制品细菌多样性. 食品工业科技. 2022(08): 133-140 . 本站查看
9.	赵野，刘艳，周晓洁，钱鑫，张家铭，周文化. 腌制技术对芥菜品质影响的研究进展. 粮食与油脂. 2022(09): 22-26 .