Determination of Isomaltooligosaccharide in Foods by Ion Chromatography-pulsed Amperometric Detection

XU Jiajia; LIU Yufeng; KONG Fanhua; QIAO Zichun; CUI Yajuan; XU Xiyuan; ZHENG Xiaowei; LI Dong

doi:10.13386/j.issn1002-0306.2020050223

Volume 42 Issue 13

Jun. 2021

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Science and Technology of Food Industry > 2021 > 42(13): 262-267. > DOI: 10.13386/j.issn1002-0306.2020050223

XU Jiajia, LIU Yufeng, KONG Fanhua, et al. Determination of Isomaltooligosaccharide in Foods by Ion Chromatography-pulsed Amperometric Detection[J]. Science and Technology of Food Industry, 2021, 42(13): 262−267. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020050223.

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Determination of Isomaltooligosaccharide in Foods by Ion Chromatography-pulsed Amperometric Detection

Beijing Institute of Nutrition Resources, Beijing 100069, China

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Received Date: May 19, 2020
Available Online: April 24, 2021

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Abstract

Objective: An ion chromatography - pulsed amperometric detection method was developed for the determination of isomaltose, isomaltotriose, panose in foods. Methods: Samples were extracted by water and the protein in sample was deposited by acid, and purified with the column of guard ⅡRP, then those sugars were separated on a CarboPac PA20 high performance anion-exchange column of ThermoFisher. The water(A), 200 mmol/L sodium hydroxide solution (B) were used as mobile phases for gradient elution, and pulsed amperometric detector was used for quantitative determination. Results: The linear relationship was good within the range of 0.8~16.0 mg/L, the correlation coefficient was more than 0.999, the RSD of precision of isomaltose、isomaltotriose and panose were 3.42%, 2.34%, 1.11%, the recovery rate was 85.63%~105.22%, the RSD was 2.48%~4.49%. Conclusion:This method demonstrated to be simple with good accuracy and specifity, which is suitable for the determination of isomaltooligosaccharide in kinds of foods.
- isomaltose,
- isomaltotriose,
- panose,
- ion chromatography

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References

[1]	杜慧真, 程振倩, 王晓华, 等. 功能性低聚糖与人类健康[J]. 中华现代临床医学杂志,2010,8(3):125−160.
[2]	尤新. 功能性低聚糖的生产与应用[M]. 北京: 中国轻工业出版社, 2004: 52−60.
[3]	金茂国, 金屹. 蛋糕加工工艺 [M]. 第六版.北京: 中国轻工业出版社, 2004: 199−200.
[4]	Koh D, Park J, Lim J, et al. A rapid method for simultaneous quantification of 13 sugars and sugar alcohols in food products by UPLC-ELSD[J]. Food Chemistry,2018,240:694−700. doi: 10.1016/j.foodchem.2017.07.142
[5]	张雪松, 王国栋, 王竹. 离子色谱一脉冲安培检测法测定食品中的聚葡萄糖[J]. 食品安全质量检测学报,2019,10(10):2856−2860. doi: 10.3969/j.issn.2095-0381.2019.10.006
[6]	中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. GB/T 20881-2017 低聚异麦芽糖[S]. 北京: 中国标准出版社, 2017.
[7]	傅博强, 王晶, 唐治玉, 等. 食品中低聚异麦芽糖高效液相色谱检测方法研究[J]. 食品工业科技,2010,41(10):388−391.
[8]	王良东. 低聚异麦芽糖性质、功能、生产和应用[J]. 粮食与油脂,2018(4):43−47. doi: 10.3969/j.issn.1008-9578.2018.04.012
[9]	单黎然, 龚月桦, 贾建光, 等. 4种重要功能性低聚糖的研究进展[J]. 西北农林科技大学学报,2006,34(7):96−100.
[10]	Bouhnik Y, Raskine L, Simoneau G, et al. The capacity of nondigestible carbohydrates to stimulat efecal bifidobacteria in healthy humans: Adouble-blind, randomized, placebo-cont roll ed, parallel-group, dose-response relation study[J]. American Journal of Clinical Nutrition,2004,80(6):1658−1664. doi: 10.1093/ajcn/80.6.1658
[11]	杨海军. 低聚异麦芽糖的应用[J]. 山东食品发酵,2007(3):33−36.
[12]	毕颖, 刘燕, 张平, 等. 蒽酮比色法测定大豆乳清废水中总糖含量[J]. 大豆通报,2006(3):24−25.
[13]	任一平, 铁晓威, 黄百芬, 等. 毛细管电泳法测定食品中的低聚糖异麦芽糖含量[J]. 食品工业科技,2000,21(6):19−24.
[14]	林家永. 异麦芽低聚糖含量测定方法的研究[J]. 食品工业科技,2009,30(6):351−354.
[15]	吴红京, 唐根源, 黄志, 等. 测定异麦芽低聚糖组分的高效液相色谱法[J]. 分析测试学报,2002,21(1):63−64. doi: 10.3969/j.issn.1004-4957.2002.01.019
[16]	陈志颖, 徐正康, 郭峰, 等. 液体糖中还原糖含量测定方法的研究[J]. 食品工程,2018(1):47−52. doi: 10.3969/j.issn.1673-6044.2018.01.012
[17]	梁智安, 王成龙, 龙飞. 液相色谱示差折光法测定酒中的总糖和还原糖[J]. 食品安全质量检测学报,2018,9(9):2188−2194. doi: 10.3969/j.issn.2095-0381.2018.09.035
[18]	Zhang Wei, He Hongbo, Zhang Xudong. Determination of neutral sugars in soil by capillary gas chromatography after derivatization to aldononitrile acetates[J]. Soil Biology & Biochemistry,2007,39:2665−2669.
[19]	Loos R, Gawlik B M, Boettcher K, et al. Sucralose screening in European surface waters using a solid phase extraction liquid chromatography-triple quadrupole mass spectrometry method[J]. Journal of Chromatography A,2009,1216:1126−1130. doi: 10.1016/j.chroma.2008.12.048
[20]	张晓萍, 段钢. 高效阴离子交换色谱-脉冲安培法检测低聚异麦芽糖[J]. 食品与发酵工业,2012,38(7):144−147.
[21]	Luca F A, Dolores M, Aranzazu G, et al. Transformation of maltose into prebiotic isomaltooligosaccharides by a novel &-glucosidase from Xantophyllomyces dendrorhous[J]. Process Biochemistry,2007,42:1531−1536.
[22]	Max F, Jinadevi S, Audrey A. Determination of complex polysaccharides by HPAE-PAD in foods: Validation using accuracy profile[J]. Journal of Chromatography B,2009,877:2388−2395. doi: 10.1016/j.jchromb.2008.10.004
[23]	Greya C, Edebrinka P, Krooka M, et al. Development of a high performance anion exchange chromatography analysis for mapping of oligosaccharides[J]. Journal of Chromatogra-phy B,2009,877:1827−1832. doi: 10.1016/j.jchromb.2009.05.003
[24]	宋卫得, 苏征, 惠稀东, 等. 离子色谱-积分脉冲安培检测法同时测定酱油中[J]. 色谱,2019,37(9):996−1003.
[25]	朱叶青, 吕娟, 王婷婷, 等. 离子色谱法同时测定蛋白胨中葡萄糖、果糖、蔗糖、麦芽糖、乳糖含量[J]. 食品安全质量检测学报2019, 10(14): 4624−4628.
[26]	乐胜锋, 王尉, 王雅萱, 等. 离子色谱-脉冲安培法测定芦荟多糖中7种单糖的含量[J]. 色谱,2019,37(3):319−324.
[27]	邹学权, 傅飞霞, 陈孜. 离子色谱-脉冲安培法同时测定婴幼儿配方食品中的蔗糖与乳糖[J]. 浙江化工,2018,49(3):48−51. doi: 10.3969/j.issn.1006-4184.2018.03.013
[28]	朱伟, 宁啸骏, 杜茹芸. 高效离子色谱法检测婴幼儿配方奶粉中的功能性低聚糖[J]. 食品科学,2017,38(24):224−228. doi: 10.7506/spkx1002-6630-201724036
[29]	李晓磊, 胡才龙, 李丹, 高效阴离子交换色谱测定糖浆中异麦芽糖含量[J]. 食品研究与开发, 2013, 34(3): 56-58.
[30]	巫广华, 吴家玲, 雷艳, 等. 离子色谱法-脉冲安培检测器测定奶粉中果聚糖的含量[J]. 食品与发酵科技,2019,55(4):112−115.
[31]	冉丹, 张燕俊, 袁列江, 等. 离子色谱法检测婴幼儿配方奶粉中低聚半乳糖含量[J]. 食品研究与开发,2019(4):148−153. doi: 10.3969/j.issn.1005-6521.2019.04.028
[32]	杨春芳. 超市常见含糖食品中糖含量分析[J]. 分析检测, 2020(6):139-140,146..
[33]	彭丽诗, 陈琼, 黄盼, 等. 离子色谱-脉冲安培法同时测定食品中9 种糖和糖醇的含量[J]. 食品工业科技, 2020, 41(9):250-254,259.
[34]	汪少芸. 蛋白质纯化与分析技术[M]. 北京: 中国轻工业出版社, 2016: 65−66.
[35]	黎小兰, 陈彩云, 蔡伟江, 等. 液相色谱-质谱法测定保健食品乳清蛋白粉类中叶酸含量的前处理条件的优化[J]. 食品安全质量检测学报,2017,8(8):3085−3091. doi: 10.3969/j.issn.2095-0381.2017.08.041
[36]	中华人民共和国国家卫生和计划生育委员会, 国家食品药品监督管理总局. GB 5009.89-2016 食品安全国家标准食品中烟酸和烟酰胺的测定[S]. 北京: 中国标准出版社, 2016.
[37]	张水锋, 盛华栋, 姜侃, 等. 梯度洗脱优化-离子色谱-脉冲安培法分析婴幼儿配方乳粉中的糖和糖醇[J]. 色谱,2016,34(10):946−950.

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