Determination of Isomaltooligosaccharide in Foods by Ion Chromatography-pulsed Amperometric Detection

XU Jiajia; LIU Yufeng; KONG Fanhua; QIAO Zichun; CUI Yajuan; XU Xiyuan; ZHENG Xiaowei; LI Dong

doi:10.13386/j.issn1002-0306.2020050223

Volume 42 Issue 13

Jun. 2021

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Science and Technology of Food Industry > 2021 > 42(13): 262-267. > DOI: 10.13386/j.issn1002-0306.2020050223

XU Jiajia, LIU Yufeng, KONG Fanhua, et al. Determination of Isomaltooligosaccharide in Foods by Ion Chromatography-pulsed Amperometric Detection[J]. Science and Technology of Food Industry, 2021, 42(13): 262−267. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020050223.

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Determination of Isomaltooligosaccharide in Foods by Ion Chromatography-pulsed Amperometric Detection

Beijing Institute of Nutrition Resources, Beijing 100069, China

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Received Date: May 19, 2020
Available Online: April 24, 2021

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Abstract

Objective: An ion chromatography - pulsed amperometric detection method was developed for the determination of isomaltose, isomaltotriose, panose in foods. Methods: Samples were extracted by water and the protein in sample was deposited by acid, and purified with the column of guard ⅡRP, then those sugars were separated on a CarboPac PA20 high performance anion-exchange column of ThermoFisher. The water(A), 200 mmol/L sodium hydroxide solution (B) were used as mobile phases for gradient elution, and pulsed amperometric detector was used for quantitative determination. Results: The linear relationship was good within the range of 0.8~16.0 mg/L, the correlation coefficient was more than 0.999, the RSD of precision of isomaltose、isomaltotriose and panose were 3.42%, 2.34%, 1.11%, the recovery rate was 85.63%~105.22%, the RSD was 2.48%~4.49%. Conclusion:This method demonstrated to be simple with good accuracy and specifity, which is suitable for the determination of isomaltooligosaccharide in kinds of foods.
- isomaltose,
- isomaltotriose,
- panose,
- ion chromatography

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