Optimization of the Extration Technology of Total Saponins from <i>Stauntonia chinensis</i> DC. and the Selection of Its Whitening Active Components

CAO Yingkun; LIU Zuwang; QIU Fengmei; HUANG Zhen; ZHONG Xiaoming

doi:10.13386/j.issn1002-0306.2020050177

Volume 42 Issue 5

Feb. 2021

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Science and Technology of Food Industry > 2021 > 42(5): 152-158,165. > DOI: 10.13386/j.issn1002-0306.2020050177

CAO Yingkun, LIU Zuwang, QIU Fengmei, HUANG Zhen, ZHONG Xiaoming. Optimization of the Extration Technology of Total Saponins from Stauntonia chinensis DC. and the Selection of Its Whitening Active Components[J]. Science and Technology of Food Industry, 2021, 42(5): 152-158,165. DOI: 10.13386/j.issn1002-0306.2020050177

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Optimization of the Extration Technology of Total Saponins from Stauntonia chinensis DC. and the Selection of Its Whitening Active Components

Zhejiang Chinese Medical University, Hangzhou 310053, China

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Received Date: May 17, 2020
Available Online: March 02, 2021

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Abstract

Objective:To optimize the extraction process of total saponins from Stauntonia chinensis DC.and lay a foundation for the further development and application of total saponins in whitening cosmetics. Methods:Based on the single factor experiment,the total saponins yield was optimized by ethanol concentration,liquid to material ratio,extraction temperature and extraction time. The extraction process of total saponins was optimized by Box-Behnken method.The extract of Stauntonia chinensis DC. was extraced by solvents with different polarity,and separated by chromatography to obtain different active components.The proliferation inhibition ability was detected by MTT assay and the tyrosinase activity was detected by Dopa oxidation method. Results:The order of the factors affecting the total saponins was:Ethanol concentration > extraction temperature > liquid to material ratio > extraction time. The optimal conditions were determined to be 79% for ethanol concentration,25:1 mL/g for liquid to material ratio,81 ℃ for extraction temperature,89 min for extraction time,the average yield of total saponins was 2.16%. The n-butanol site had the best inhibition rate on proliferation of B16 cells and tyrosinase activity,which was better than control arbutin(P<0.05),and IC₅₀ were 86.61 and 69.09 μg/mL. A total of 8 active components were obtained from n-butanol. Fr.6 had the best inhibitory effect on B16 cells and tyrosinase activity,of which the IC₅₀ were 80.93 and 58.99 μg/mL,and inhibition effect of tyrosinase was better than n-butanol site before separation(P<0.05). Conclusion:Response surface methodology was used to determine the optimal extraction process of total saponins and the Fr.6 had good whitening activity. This study shows that the Stauntonia chinensis DC. has a good application prospect in the development of whitening cosmetics.
- total saponins from Stauntonia chinensis DC.,
- extraction process,
- response surface,
- whitening activity

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[1]	Wang H B,Yu D Q,Liang X T. Structures of two nortriterpenoidsaponins from Stauntonia chinensis[J]. Journals of Natural Products,1990,53(2):313-318.
[2]	蒋纬,胡颖. 野木瓜活性成分及应用研究进展[J]. 食品工程,2018(3):4-6,38.
[3]	国家药典委员会. 中华人民共和国药典[M]. 北京:中国医药科技出版社,2015:314.
[4]	Chen S,Rong Y,Liu M X,et al. Analgesic effects of triterpenoid saponins from Stauntonia chinensis via selective increase in inhibitory synaptic response in mouse cortical neurons[J]. Frontiers in Pharmacology,2018(9):1302.
[5]	叶文博,张慧绮,金荣华. 野木瓜皂甙对大鼠神经髓鞘和轴突膜的作用[J]. 神经解剖学杂志,2003(3):311-316.
[6]	祝小波,李宇婷,朱欣婷,等. 野木瓜果实总皂苷体外抗肿瘤作用研究[J]. 安徽医药,2017,21(6):991-994.
[7]	王知松,刘金娥,修慧迪,等. 野木瓜水溶性粗提物工艺优化及美白效果分析[J]. 贵州农业科学,2017,45(8):99-103.
[8]	崔霖芸. 野木瓜提取液中黄酮的测定及其体外抗氧化研究[J]. 食品研究与开发,2014,35(20):94-97.
[9]	夏季,郑炯,陈光静,等. 响应面法优化超声辅助提取野木瓜多酚工艺[J]. 食品工业科技,2014,35(21):253-258.
[10]	王文平,田亮,吴国卿,等. 野木瓜水溶性多糖的分离纯化及抗氧化活性研究(英文)[J]. 天然产物研究与开发,2014,26(5):745-749 ,769.
[11]	宋发军,杨芳,许德红,等. 野木瓜藤茎部位总皂苷闪式提取工艺[J]. 中南民族大学学报(自然科学版),2010,29(3):29-32.
[12]	张婷婷,李静,王成永. 木瓜总皂苷提取工艺研究[J]. 安徽医药,2009,13(2):143-144.
[13]	唐维媛,张义明,董永刚. HPLC法同时测定野木瓜中齐墩果酸与熊果酸的研究[J]. 中国食品添加剂,2010(4):196-200.
[14]	张天啸,付辉政,杨毅生,等. 野木瓜化学成分研究[J]. 中药材,2016,39(7):1554-1558.
[15]	秦亚东,周娟娟,汪荣斌. 白芍多糖抑制酪氨酸酶活性成分的筛选[J]. 中成药,2018,40(12):2755-2758.
[16]	盛晓笑,王春丽. 蔷薇花总黄酮对酪氨酸酶的抑制作用及其动力学行为[J]. 中成药,2018,40(1):97-100.
[17]	Shi F Y,Xie L W,Lin Q L,et al. Profiling of tyrosinase inhibitors in mango leaves for a sustainable agro-industry[J]. Food Chemistry,2020,312.
[18]	李志英,王雪寒,高瑞苑. 微波提取白茯苓美白成分的工艺研究[J]. 日用化学工业,2019,49(4):238-243.
[19]	冯童童,付辉政,杨毅生,等. 野木瓜茎枝三萜皂苷类化学成分研究[J]. 中国现代中药,2018,20(5):533-539.
[20]	唐维媛. 野木瓜生理活性成分的HPLC研究[D]. 贵阳:贵州大学,2007.
[21]	Wang D,Tian J,Zhou G P,et al. Triterpenoid glycosides from Stauntonia chinensis[J]. J Asian Nat Prod Res,2010,12(2):150-156.
[22]	努尔买买提·艾买提,哈木拉提·吾甫尔,吐尔逊·吾甫尔.异常黏液质成熟剂对B16黑色素瘤细胞的增殖、酪氨酸酶活性、黑色素的合成的影响[J]. 中成药,2007(12):1824-1826.
[23]	李敏晶,姚珊珊,刘远. 响应面分析法优化砂海星皂苷提取工艺研究[J]. 安徽农业科学,2020,48(9):182-185 ,189.
[24]	赵立春. 人参化学成分的提取及测试方法的优化研究[D]. 北京:中国农业科学院,2019.
[25]	韩燕燕,张瑞,姜涛,等. 响应面法优化超高压提取人参花总皂苷工艺研究[J]. 特产研究,2019,41(3):1-7.
[26]	李洪德,赵超,蒋政萌,等. 舞花姜根总皂苷的提取工艺及其抗氧化活性[J]. 食品工业科技,2018,39(1):227-234.
[27]	林国荣,吴毕莎,苏彩凤. 响应面法优化超声提取杏鲍菇皂苷[J]. 食品工业科技,2018,39(16):1-5 ,12.
[28]	冯印,高超,于钧宇,等. 款冬总皂苷提取优化及其降脂抗氧化作用研究[J]. 食品工业,2018,39(6):169-174.
[29]	苏永昌,刘淑集,阮伟达,等. 响应面法优化海地瓜皂苷提取工艺[J]. 食品工业科技,2016,37(21):205-209 ,215.
[30]	肖珊,罗姣,张斌贝,等. 响应面法优化吴茱萸五加叶柚皮苷纯化工艺[J]. 中草药,2019,50(4):868-874.
[31]	梁桂娟. 野木瓜总皂苷的分离纯化及抗氧化活性研究[D].贵阳:贵州大学,2007.
[32]	陆秋艳. 野木瓜中黄酮和皂甙类化合物的分离提纯及其分析测试研究[D].上海:上海师范大学,2007.
[33]	赵钰,刘春莹,赵妍,等. 人参果浆中人参皂苷Re的提取和生物转化[J]. 食品工业科技,2020,41(4):25-30.
[34]	王仁广,杨净尧,张欣舒,等. 柴胡中柴胡皂苷a、柴胡皂苷d的电磁裂解水提取工艺优化[J]. 中国药房,2019,30(18):2546-2551.

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Optimization of the Extration Technology of Total Saponins from Stauntonia chinensis DC. and the Selection of Its Whitening Active Components