Optimized the Process of Enzymes Coupled with <i>Lactobacillus plantarum</i> and <i>Saccharomyces cerevisiae</i> to Ferment Ginkgo Seeds by Response Surface Methodology

YAO Fang; ZHANG Wei; XU Haixiang; WANG Hailan; GUAN Peng

doi:10.13386/j.issn1002-0306.2020050116

Volume 42 Issue 4

Feb. 2021

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Science and Technology of Food Industry > 2021 > 42(4): 77-85. > DOI: 10.13386/j.issn1002-0306.2020050116

YAO Fang, ZHANG Wei, XU Haixiang, WANG Hailan, GUAN Peng. Optimized the Process of Enzymes Coupled with Lactobacillus plantarum and Saccharomyces cerevisiae to Ferment Ginkgo Seeds by Response Surface Methodology[J]. Science and Technology of Food Industry, 2021, 42(4): 77-85. DOI: 10.13386/j.issn1002-0306.2020050116

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Optimized the Process of Enzymes Coupled with Lactobacillus plantarum and Saccharomyces cerevisiae to Ferment Ginkgo Seeds by Response Surface Methodology

Department of Food Science and Technology, Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China

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Received Date: May 10, 2020
Available Online: March 01, 2021

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Abstract

Abstract

This study aimed to optimize the process of enzymes coupled with Lactobacillus plantarum and Saccharomyces cerevisiae to ferment ginkgo seeds by response surface methodology,in order to obtain a kind of ginkgo fermentation powder with high antioxidant activity. The effects of solid-liquid ratio,total amount of compound enzyme,mass ratio of glucoamylase and α-amylase,enzymolysis time,total amount of mixed fermentation agent,mass ratio of Lactobacillus plantarum and Saccharomyces cerevisiae,fermentation time on soluble protein content and DPPH radical scavenging capacity of ginkgo fermentation powder(GFP)were studied by single factor experiment,and based on the DPPH radical scavenging capacity,response surface analysis was used to optimize the process conditions of Lactobacillus plantarum and Saccharomyces cerevisiae coupled with enzymes to ferment ginkgo seeds. The results showed that on the basis of adding 0.3 g/100 g of compound enzyme,enzymolysis 2 h and fermentation 12 h,the best technology of cooperation of bacteria and enzyme fermentation was as follows:1:6 of solid-liquid ratio,2:1 of glucoamylase and α amylase,3.12 g/100 g of mixed fermentation agent,1:1 of Lactobacillus plantarum and Saccharomyces cerevisiae,the DPPH radical scavenging capacity was 69.7%±1.1%,which was basically consistent with the model prediction. Compared with the untreated ginkgo powder(G),the DPPH free radical scavenging rate of Ginkgo fermentation powder(GFP)increased by 257.4%,the soluble protein content was 4.47±0.13 g/100 g,increased by 330%,the flavone content was 0.15±0.01 mg/g,increased by 50%,the polyphenol content was 2.49±0.04 mg/g,increased by 118%,the ginkgolic acid content was 2.96±0.32 μg/g,the removal rate was 85.4%. It showed that enzymes coupled with Lactobacillus plantarum and Saccharomyces cerevisiae fermentation could significantly improve the antioxidant activity of ginkgo seeds,and the contents of antioxidant components as soluble protein,flavonoids and polyphenols in the GFP were significantly increased,and the contents of toxic components ginkgolic acid was significantly decreased,which provided the material basis for the deep processing of ginkgo seeds.
- ginkgo seeds,
- enzyme and bacteria synergy,
- biological fermentation,
- antioxidant activity,
- Lactobacillus plantarum

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[1]	Wang H Y,Zhang Y Q. The main active constituents and detoxification process of Ginkgo biloba seeds and their potential use in functional health foods(Review)[J]. Journal of Food Composition and Analysis,2019,83:103247.
[2]	Wu C E,Jia S Q,Fan G J,et al. Purification and identification of novel antioxidant peptides from enzymatic hydrolysate of Ginkgo biloba seed proteins[J]. Food Science and Technology Research,2013,19(6):1029-1035.
[3]	Hyun K. Hypocholesterolemic effect of Ginkgo biloba seeds extract from high fat diet mice[J]. Biomedical Science Letters,2017,23(2):138-143.
[4]	周晓辉,王瑱,邱立娟,等.银杏果提取物抗氧化及抗菌研究[J]. 时珍国医国药,2018,29(3):577-580.
[5]	Shugo H,Yumi K,Toshikazu S,et al. Anti-obesity effect of ginkgo vinegar,a fermented product of ginkgo seed coat,in mice fed a high-fat diet and 3T3-L1 preadipocyte cells[J]. Nutrients,2020,12(1):230.
[6]	黄文,谢笔钓,王益,等.白果蛋白质的分离、纯化、理化特性及其抗氧化活性研究[J]. 中国农业科学,2004(10):1537-1543.
[7]	Huang W,Deng Q C,Xie B J. Purification and characterization of an antioxidant protein from Ginkgo biloba seeds[J]. Food Research International,2010,43(1):86-94.
[8]	Guo Na,Song Xin-Rui,Kou Ping,et al. Optimization of fermentation parameters with magnetically immobilized Bacillus natto on ginkgo seeds and evaluation of bioactivity and safety[J]. LWT-Food Science & Technology,2018,97:172-179.
[9]	Y Niu,J F Zhang,X L Wan,et al. Effect of fermented Ginkgo biloba leaves on nutrient utilisation,intestinal digestive function and antioxidant capacity in broilers[J]. British Poultry Science,2019,60(1):47-55.
[10]	Wang Yuchen,Tao Yang,Zhang Xinyan,et al. Metabolic profile of ginkgo kernel juice fermented with lactic aicd bacteria:A potential way to degrade ginkgolic acids and enrich terpene lactones and phenolics[J]. Process Biochemistry,2019,76:25-33.
[11]	任金玫.发酵法脱除银杏酸效应的研究[D].西安:陕西科技大学,2015.
[12]	De F I,Pinon N,Maubois J L,et al. The addition of a cocktail of yeast species to Cantalet cheese changes bacterial survival and enhances aroma compound formation[J]. International Journal of Food Microbiology,2009,129(1):37-42.
[13]	段小果,李博,贺银凤.乳酸菌与酵母菌共生机理的研究进展[J]. 微生物学通报,2017,44(8):1988-1995.
[14]	周昊,王成章,叶建中,等.混菌固态发酵银杏叶渣生产蛋白饲料的研究[J]. 饲料工业,2014(S1):90-95.
[15]	Xiaoyan Liu,Guanjun Cao,Qin Wang,et al. The effect of Bacillus coagulans-fermented and nonfermented Ginkgo biloba on the immunity status of broiler chickens[J]. Journal of Animal Science,2015,93(9):3384-3394.
[16]	柳荫,吴凤智,陈龙,等.考马斯亮蓝法测定核桃水溶性蛋白含量的研究[J]. 中国酿造,2013,32(12):131-133.
[17]	王希.大麦多糖的提取及其生物活性研究[D].镇江:江苏大学,2008.
[18]	王荞薇,谢媛媛,王义明,等.银杏叶中银杏酚酸类成分含量测定方法研究[J]. 中国药学杂志,2015,50(2):167-173.
[19]	祝莹.苦瓜冻干超微粉对代谢综合征的干预作用研究[D].镇江:江苏大学,2013.
[20]	姚芳,肖香,董英.大麦乳酸菌发酵液粉中多酚的提取及其抗氧化性研究[J]. 食品工业科技,2017,38(10):211-216.
[21]	马琦媛.不同发酵对小米中植酸、氨基酸和矿物质消化率的影响[D].北京:中国农业大学,2013.
[22]	Bartolome B,Gòmez-Cordovés C. Barley spent grain:Release of hydroxycinnamic acids(ferulic and p-coumaric acids)by commercial enzyme preparations[J]. Journal of the Science of Food and Agriculture,1999,79(3):435-439.
[23]	陈东方.酶解提高燕麦粉抗氧化活性的作用机制[D].咸阳:西北农林科技大学,2016.
[24]	熊涛,黄锦卿,宋苏华,等.植物乳杆菌发酵培养基的优化及其高密度培养技术[J]. 食品科学,2011,32(7):262-268.
[25]	薛淑龙,范昊安,陈小伟,等.竹叶酵素发酵过程中代谢产物及抗氧化活性的变化[J]. 现代食品科技,2019,35(5):228-235 ,174.
[26]	刘巧林.液态发酵茶工艺参数及相关酶活性的研究[D]. 成都:四川农业大学,2007.
[27]	郭宏垚,李冬,雷雄,等.花椒多酚提取工艺响应面优化及动力学分析[J]. 食品科学,2018,39(2):247-283.
[28]	Zhou H,Wang C Z,Ye J Z,et al. Solid-state fermentation of Ginkgo biloba L. residue for optimal production of cellulase,protease and the simultaneous detoxification of Ginkgo biloba L. residue using Candida tropicalis and Aspergillus oryzae[J]. European Food Research and Technology,2015,240(2):379-388.
[29]	Ganzle M G,Vermeulen N,Vogel R F. Carbohydrate,peptide and lipid metabolism of lactic acid bacteria in sourdough[J]. Food Microbiology,2007,24(2):128-138.
[30]	刘慧菊,韩丽娟,乔杨波,等.不同微生物液态发酵对蚕豆蛋白营养价值及功能特性的影响[J]. 食品与发酵工业,2020,46(4):65-71.
[31]	Wang Jun,Wang Chun-Jie,Zhao Wen-Yong,et al. Studies on the extracting technology of flavones in ginkgo leaves assisted by microwave[J]. Advance Journal of Food Science and Technology,2016,10(4):254-256.

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