Antioxidant,Anti-inflammatory and Immunomodulatory Effects of Abalone Hydrolytic Peptide

Abalone hydrolytic peptide(AHP)was prepared from abalone meat by complex enzymatic method,and its anti-inflammatory and immunity enhancing effects were discussed. Method:The antioxidant of AHP was studied and free radical scavenging ability was taken as index,the anti-inflammatory effect of mouse alveolar macrophages(MH-S)was studied by interleukin and tumor necrosis factor-α(TNF-α),and the body weight,organ/body weight ratio,delayed allergy,carbon clearance and NK cell activity of mice were measured to study the effect of AAP(0.02,0.10 and 0.20 g/kg,respectively)on immune function. Results:AHP had strong ability to scavenge DPPH free radical,ABTS free radical and superoxide anion free radical,the scavenging rates of DPPH free radical and superoxide anion free radicals by 1.0 mg/mL AHP were 84.06% and 69.74%,respectively,and the scavenging rate of ABTS free radical by 0.10 mg/mL AHP was 62.41%. 10 μg/mL AP group significantly inhibited the secretion of IL-1 β,IL-6 and TNF-α in MH-S cells induced by LPS(P<0.05),50,100 μg/mL AP group were highly significant(P<0.01). Middle dose AHP group significantly increased NK cell activity(P<0.05),high dose AP group significantly promoted monocyte macrophage carbon clearance function(P<0.05),significantly enhanced NK cell activity(P<0.01),so as to achieve immune regulation. Conclusion:The AHP has good antioxidant activity,anti-inflammatory and immunomodulatory effects.