Antioxidant Activity of Bound Phenolics from Broad Beans at Different Edible Stages

ZHANG Tianyang; CHEN Youxia; LIU Zhenzhen; LIN Lin; HONG Yue; GAO Chunyan

doi:10.13386/j.issn1002-0306.2020040316

Volume 42 Issue 4

Feb. 2021

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Science and Technology of Food Industry > 2021 > 42(4): 39-43. > DOI: 10.13386/j.issn1002-0306.2020040316

ZHANG Tianyang, CHEN Youxia, LIU Zhenzhen, LIN Lin, HONG Yue, GAO Chunyan. Antioxidant Activity of Bound Phenolics from Broad Beans at Different Edible Stages[J]. Science and Technology of Food Industry, 2021, 42(4): 39-43. DOI: 10.13386/j.issn1002-0306.2020040316

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Antioxidant Activity of Bound Phenolics from Broad Beans at Different Edible Stages

College of Public Health, Dali University, Dali 671000, China

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Received Date: April 27, 2020
Available Online: March 01, 2021

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Abstract

The antioxidant activity of bound phenolic extracts of seed coats and cotyledons at three edible stages S1(138 DAS),S2(156 DAS)and S3(173 DAS)from five broad bean varieties(Fengdou 6,13,15,17 and 18)were studied in this paper(DAS,day after seeding). The contents of total phenol,flavonoid and condensed tannin were measured by Folin-phenol method,sodium nitrite-aluminum chloride method and vanilla methanol method,respectively. The antioxidant activity was evaluated by DPPH radical scavenging activity,trolox equivalent antioxidant activity(TEAC)and iron reduction antioxidant activity(FRAP). Meanwhile,the relationship between the contents of phenolic compound and antioxidant activity was analyzed using correlation analysis. The results showed that the contents of total phenol,flavonoid,condensed tannin and antioxidant activity of seed coats were higher than those of cotyledons. Phenol contents and antioxidant activity of Fengdou 18 harvested from S2 were the highest in seed coats and cotyledons,while those of Fengdou 17 harvested from S1 were the lowest in seed coats and S3 were the lowest in cotyledons. There had differences in phenol content and antioxidant activity among different varieties during the whole harvesting process. In seed coats,(contents of total phenol)TPC and(contents of flavonoid)FC were significantly correlated with DPPH and FRAP(P<0.01),(contents of condensed tannin)CTC was significantly correlated with DPPH,TEAC and FRAP(P<0.01),and in cotyledons,TPC and FC were significantly correlated with DPPH,TEAC and FRAP(P<0.01).
- broad bean,
- bound phenolics,
- edible stage,
- flavonoid,
- antioxidant activity

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