Process Optimization of Protein Extraction from Moringa oleifera Leaf by Enzymatic Hydrolysis

In order to optimize the extraction process of cellulase and pectinase hydrolysis to extract protein from Moringa oleifera leaf,the effects of five processing conditions on the extraction rate were explored by single factor and orthogonal array design methods. Results showed that the effect of five processing conditions on the extraction rate of Moringa oleifera leaf protein with cellulase was in the descending order of reaction temperature,substrate concentration,pH,hydrolysis time and enzyme dosage. The optimal hydrolysis conditions were found to be reaction at 50 ℃ and pH5.0 for 70 min with a substrate concentration of 7.0 g/L and an enzyme dosage of 800 U/g,the extraction rate reached 43.85%. In the case of hydrolysis with pectinase,the decreasing order of five processing conditions was enzyme dosage,substrate concentration,hydrolysis time,temperature,and pH,and their optimal levels which provided maximum extraction rate as high as 32.26% were determined as 1400 U/L,9.0 g/L,50 min,50 ℃,and 4.0,respectively. In both cases,the effect of five processing conditions on the extraction rate reached up to a significant level(P<0.01). Under the optimized hydrolysis conditions,cellulase was superior to pectinase in extracting proteins from Moringa oleifera leaf.