Application of Fe3O4@ZrO2 Magnetic Nanoparticles in the Selective Enrichment of Casein Phosphopeptides

PAN Yuting; YANG Jing; YAN Xinyu; LI Na

doi:10.13386/j.issn1002-0306.2020030404

Volume 42 Issue 20

Oct. 2021

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Science and Technology of Food Industry > 2021 > 42(20): 223-228. > DOI: 10.13386/j.issn1002-0306.2020030404

PAN Yuting, YANG Jing, YAN Xinyu, et al. Application of Fe₃O₄@ZrO₂ Magnetic Nanoparticles in the Selective Enrichment of Casein Phosphopeptides[J]. Science and Technology of Food Industry, 2021, 42(20): 223−228. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020030404.

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Application of Fe₃O₄@ZrO₂ Magnetic Nanoparticles in the Selective Enrichment of Casein Phosphopeptides

Zhejiang J&A Testing Technology Co., Ltd., Hangzhou 310053, China

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Received Date: March 29, 2020
Available Online: August 21, 2021

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Abstract

Abstract

In this study, Fe₃O₄@ZrO₂ magnetic nanoparticles which were synthesized successfully were used as carrier for the highly selective enrichment of phosphopeptides from tryptic digest product of casein . Using N/P (molar ratio) of CPP and magnetic nanoparticles adsorbance as evaluating indicators, the influence of five variables which were the hydrolysis degree of casein, adsorption pH, adsorption time, adsorption temperature, and the initial concentration of phosphopeptides, on the ability of Fe₃O₄@ZrO₂ magnetic nanoparticles to isolate and enrich phosphopeptides was analyzed. The technical parameters of CPP enrichment were optimized. Results showed that, the optimized conditions of CPP enrichment were as below: Casein hydrolysis degree 22%, adsorption pH4.5, adsorption temperature 30 ℃, adsorption time 50 min, the initial concentration of phosphopeptides 50 mg/mL. Finally, under these conditions, the N/P (molar ratio) of CPP was 4.87, and the adsorbance of Fe₃O₄@ZrO₂ magnetic nanoparticles was 94.37. The bound CPP were eluted using NaOH (pH13) and the elution rate was 95% at least. Experiment results demonstrate that ZrO₂ coated-magnetic microspheres showed the excellent potential for selective enrichment of CPP, which would play an important role in the production of high quality and purity CPP.
- Fe₃O₄@ZrO₂ magnetic nanoparticles,
- selective enrichment,
- casein phosphopeptides,
- condition optimization

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[1]	Sun Shengwei, Liu Fei, Liu Guo, et al. Effects of casein phosphopeptides on calcium absorption and metabolism bioactivityin vitro and in vivo[J]. Food & Function,2018,9(10):5220−5229.
[2]	王永胜, 梅春升, 马力保, 等. 酪蛋白磷酸肽的生理功能及其促进动物机体对矿物质吸收说的机理[J]. 中国饲料,2007(15):8−11. [Wang Yongsheng, Mei Chunsheng, Ma Libao, et al. The physiofunction and the mechanism of enhancing animals to absorb minerals of casein phosphopeptides[J]. China Feed,2007(15):8−11. doi: 10.3969/j.issn.1004-3314.2007.15.004
[3]	田彦梅, 张园, 叶青, 等. 益生菌发酵羊乳中酪蛋白磷酸肽的持钙能力和结合铁能力的研究[J]. 食品与发酵科技,2019,55(4):18−22. [Tian Yanmei, Zhang Yuan, Ye Qing, et al. The calcium holding and iron binding capacity of caseinphosphopeptides in fermented goat milk[J]. Food and Fermentation Sciences & Technology,2019,55(4):18−22.
[4]	Philip N, Walsh L. The potential ecological effects of casein phosphopeptide-amorphous calcium phosphate in dental caries prevention[J]. Australian Dental Journal,2019,64:66−71. doi: 10.1111/adj.12661
[5]	Elgamily H, Safwat E, Soliman Z, et al. Antibacterial and remineralization efficacy of casein phosphopeptide, glycomacropeptide nanocomplex, and probiotics in experimental toothpastes: An in vitro comparative study[J]. European Journal of Dentistry,2019,13(3):391−398.
[6]	Carmen Llena, Irene Esteve, Francisco Javier Rodríguez-Lozano, et al. The application of casein phosphopeptide and amorphous calcium phosphate with fluoride (CPP-ACPF) for restoring mineral loss after dental bleaching with hydrogen or carbamide peroxide: Anin vitro study[J]. Annals of Anatomy,2019, 225:48−53.
[7]	Abufarwa Moufida, Noureldin Amal, Azimaie Taha, et al. Preventive effects of carbon dioxide laser and casein phosphopeptide amorphous calcium phosphate fluoride varnish on enamel demineralization: A comparative, in vitro study[J]. Journal of Investigative and Clinical Dentistry, 2019, 10(2): e12400.
[8]	Naqvi Muhammad Ali, Irani Kimia Anaraki, Katanishooshtari Maryam, et al. Disorder in milk proteins: formation, structure, function, isolation and applications of casein phosphopeptides[J]. Current Protein & Peptide Science,2016,17(4):368−79.
[9]	焦宇知. 酪蛋白磷酸肽新生产工艺及其功能性质研究[D]. 无锡: 江南大学, 2004. Jiao Yuzhi. Studies on novel production technologies for CPP and its functional properties[D]. Wuxi: Jiangnan University, 2004.
[10]	李鹏章, 王粤博. 蛋白质组学中磷酸化肽的常用富集方法[J]. 化学进展,2012,24(9):1785−1793. [Li P Z, Wang Y B. Enrichment methods of phosphopeptides in proteomics[J]. Progress in Chemistry,2012,24(9):1785−1793.
[11]	Li Yan, Leng Taohua, Lin Huaqing, et al. Preparation of Fe₃O₄@ZrO₂ core-shell microspheres as affinity probes for selective enrichment and direct determination of phosphopeptides using matrix-assisted laser desorption ionization mass spectrometry[J]. Journal of Proteome Research,2007,6(11):4498−4510. doi: 10.1021/pr070167s
[12]	Li Xiaoshui, Wu Jianhong, Zhao Yong, et al. Preparation of magnetic polymer material with phosphate group and its application to the enrichment of phosphopeptides[J]. Journal of Chromatography A,2011,1218(25):3845−3853. doi: 10.1016/j.chroma.2011.04.044
[13]	Chen Rui, Li Jianjun. Enrichment of phosphorylated mhc peptides with immobilized metal affinity chromatography and titanium dioxide particles[J]. Methods in Molecular Biology,2019,2024:259−268.
[14]	Li Yilin, Liu Liangliang, Wu Hao, et al. Magnetic mesoporous silica nanocomposites with binary metal oxides core-shell structure for the selective enrichment of endogenous phosphopeptides from human saliva[J]. Analytica Chimica Acta,2019,1079:111−119. doi: 10.1016/j.aca.2019.06.045
[15]	Xu Xiangling, Asher Sanford A. Synthesis and utilization of monodisperse hollow polymeric particles in photonic crystals[J]. Journal of the American Chemical Society,2004,126(25):7940−7945.
[16]	黄菁菁, 徐祖顺, 易昌凤. 化学共沉淀法制备纳米四氧化三铁粒子[J]. 湖北大学学报(自然科学版),2007,29(1):50−52. [Huang Jingjing, Xu Zushun, Yi Changfeng. Fe₃O₄ nanoparticles prepared by chemical co-precipitation method[J]. Journal of Hubei University (Natural Science),2007,29(1):50−52.
[17]	孙俊. 功能性磁性纳米颗粒的制备及其在食品体系中的应用研究[D]. 无锡: 江南大学, 2012. Sun Jun. Preparation and application of functional magnetic nanoparticles in food system[D]. Wuxi: Jiangnan University, 2012.
[18]	Katrine H Ellegård, Claus Gammelgård-Larsen, Esben S Sørensen, et al. Process scale chromatographic isolation, characterization and identification of tryptic bioactive casein phosphopeptides[J]. International Dairy Journal,1999,9(9):639−652. doi: 10.1016/S0958-6946(99)00135-1
[19]	冯凤琴, 许时婴, 王璋. 酪蛋白磷酸肽(CPPs)的分子量及持钙功能的分析[J]. 食品与发酵工业,1997,23(2):18−21. [Feng Fengqin, Xu Shiying, Wang Zhang. Discussion on the molecules size and calcium-binding capacity of casein phosphopeptides[J]. Food and Fermentation Industry,1997,23(2):18−21.
[20]	刘清斌, 章俊, 许德富, 等. 酪蛋白磷酸肽制备工艺研究[J]. 中国酿造,2010(10):60−65. [Liu Qingbin, Zhang Jun, Xu Defu, et al. Preparation technology of casein phosphopeptides[J]. China Brewing,2010(10):60−65. doi: 10.3969/j.issn.0254-5071.2010.10.019
[21]	中华人民共和国国家标准. GB 5009.5-2016 食品安全国家标准食品中蛋白质的测定 [S]. National standards of People's Republic of China.GB 5009.5-2016 National Food Safety Standard. Determination of protein in food[S].
[22]	中华人民共和国国家标准. GB 5009.87-2016 食品安全国家标准食品中磷的测定[S]. National standards of People’s Republic of China.GB 5009.87-2016 National Food Safety Standard. Determination of phosphorus in food[S].
[23]	孙婷婷. 低抗原性酪蛋白磷酸肽的制备及其功能的研究[D]. 呼和浩特: 内蒙古农业大学, 2010. Sun Tingting. Studies on prodution technologies for low antigenicity CPP and its functional properties[D]. Huhhot: Inner MongoliaAgricultural University, 2010.
[24]	田波, 迟玉杰. 蛋清蛋白水解物的水解程度与分子量关系的研究[J]. 食品工业科技,2002(11):14−15. [Tian Bo, Chi Yujie. Studies on the relationship between the degree of hydrolysis of egg white protein hydrolysate and molecular weight[J]. Science and Technology of Food Industry,2002(11):14−15. doi: 10.3969/j.issn.1002-0306.2002.11.005
[25]	Ho Y S, Mc Kay G. Sorption of dyes and copper ions onto biosorbents[J]. Process Biochemistry,2003,38(7):1047−1061. doi: 10.1016/S0032-9592(02)00239-X

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