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Volume 42 Issue 1
Dec.  2020
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CHEN Sheng-jie, GAO Xiang, YUAN Rong-yu. Formulation Optimization of Freeze-Drying Protectant for Probiotics Powder by Vacuum Freeze-Drying[J]. Science and Technology of Food Industry, 2021, 42(1): 182-187,196. DOI: 10.13386/j.issn1002-0306.2020020317
Citation: CHEN Sheng-jie, GAO Xiang, YUAN Rong-yu. Formulation Optimization of Freeze-Drying Protectant for Probiotics Powder by Vacuum Freeze-Drying[J]. Science and Technology of Food Industry, 2021, 42(1): 182-187,196. DOI: 10.13386/j.issn1002-0306.2020020317
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Formulation Optimization of Freeze-Drying Protectant for Probiotics Powder by Vacuum Freeze-Drying

  • Guangdong Cleamol Technology Company Limited, Foshan 528000, China

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  • Received Date: March 01, 2020
  • Available Online: January 07, 2021
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    • Abstract
  • In order to improve the survival rate of probiotics lyophilized powder,the effects of freeze-drying protectants on the survival rate of probiotics were studied using vacuum freeze-drying mothod. The protectants included skim milk powder,xylo-oligosaccharides,soluble starch and VCsodium salt and the probiotics included Lactobacillus plantarum SC1, Bacillus coagulans XP2 and Saccharomyces cerevisiae SA1. The optimum combinations were selected by single factor and orthogonal test. With the pre-freezing temperature -70 ℃,2h,and freezing temperature -50 ℃,10 Pa,38h,the best formula combinations of protectant for L. plantarum was soluble starch 10%,VCsodium salt 3%,skim milk powder 12%,xylo-oligosaccharide 14%;the best formula combinations of protectant for B. coagulans was xylo-oligosaccharide 8%,VC sodium salt 4%,soluble starch 14%,skimmed milk powder 8%;the best formula combinations of protectant for S.cerevisiae was xylo-oligosaccharide 10%,VC sodium salt 2%,skimmed milk powder 12%,soluble starch 12%. Under the optimal formula protectant,the freeze-drying survival rate of the three strains were 83.2%,83.7% and 86.7% respectively,and the number of viable bacteria were all higher than 1.0×108 CFU/g,which has a good commercial application value.
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