Rapid Screening of Malachite Green Residues in Aquatic Products Using DBDI-MS/MS

SU Jing; JIANG Yingjie; CHEN Changyi; LIU Yang; ZHANG Wentian; CHEN Sheng

doi:10.13386/j.issn1002-0306.2019110271

Volume 42 Issue 5

Feb. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(5): 221-225,235. > DOI: 10.13386/j.issn1002-0306.2019110271

SU Jing, JIANG Yingjie, CHEN Changyi, LIU Yang, ZHANG Wentian, CHEN Sheng. Rapid Screening of Malachite Green Residues in Aquatic Products Using DBDI-MS/MS[J]. Science and Technology of Food Industry, 2021, 42(5): 221-225,235. DOI: 10.13386/j.issn1002-0306.2019110271

Citation:

PDF (1452 KB)

Rapid Screening of Malachite Green Residues in Aquatic Products Using DBDI-MS/MS

1. Huai'an Food and Drug Inspection Institute, Huai'an 223001, China;
2. Jiangsu Food Pharmaceutical Science College, Huai'an 223003, China;
3. China Innovation Instrument Co., Ltd., Ningbo 315199, China

More Information

Received Date: November 28, 2019
Available Online: March 02, 2021

Graphical Abstract

Abstract

Abstract

A rapid screening method using DBDI-MS/MS was established for MG and LMG residues in aquatic products. The samples were extracted and purified,and then injected under the conditions of ionization temperature of 230 ℃ and He gas flow rate of 3 L/min using DBDI in the positive ion mode. The determination was conducted by tandem mass spectrometry in positive ESI mode under multiple reaction monitoring(MRM)mode. Under the optimal conditions,the experimental data of MG and LMG showed a good linear relationship between the signal and the concentration in 1~20 μg/L,with correlation coefficient(r)of 0.9951 and 0.9939. Moreover,the recoveries of MG and LMG were between 61.8% and 78.4% at three spiked levels. This method without tedious pretreatment and chromatographic separation,was simple and rapid,can meet the requirement of rapid screening and analysis of large quantities of samples.

FullText(HTML)

References (22)

References

[1]	张艳,冯翠霞,陈剑刚,等.超高效液相色谱-串联质谱法测定水产品中孔雀石绿、结晶紫及其代谢物残留[J].中国卫生检验杂志,2017,27(10):1385-1388.
[2]	张艺蓓,岳田利,乔海鸥,等.超高效液相色谱-串联质谱法检测鱼中孔雀石绿、结晶紫及其代谢物[J].食品科学,2014,35(10):179-184.
[3]	Majeed S A,Nambi K S N,Taju G,et al. Cytotoxicity,genotoxicity and oxidative stress of malachite green on the kidney and gill cell lines of freshwater air breathing fish Channa striata[J].Environmental Science and Pollution Research International,2014,21(23):13539-13550.
[4]	Wan H P,Weng S P,Liang L Z,et al. Evaluation of the developmental toxicity of leucomalachite green administered orally to rats[J].Food and Chemical Toxicology,2011,49(12):3031-3037.
[5]	徐向荣,郝青,彭加喜,等.水产品中残留孔雀石绿研究进展[J].热带海洋学报,2013,32(4):97-106.
[6]	Turnipseed S B,Roybal J E,Hurlbut J A,et al. Gas chromatographic/mass spectrometric confirmation of leucomalachite green in catfish(Ictalurus punctatus)tissue[J].Journal of AOAC International,1995,78(4):971-977.
[7]	梁高道,麦琦.气相色谱质谱法确认水产品中的孔雀石绿[J].公共卫生与预防医学,2009,20(6):69-71.
[8]	李秀霞,李娇,孙协军,等.水产品中孔雀石绿及其代谢产物残留检测方法研究进展[J].食品工业科技,2015,36(3):385-388 ,393.
[9]	邓建朝,李来好,杨贤庆,等.固相萃取-高效液相色谱-荧光检测法测定水体中的孔雀石绿[J].食品科学,2012,33(14):150-153.
[10]	刘文卫,朱鹏飞,周闰,等.超高效液相色谱-串联质谱法同时检测水产品中孔雀石绿、结晶紫及其代谢物的残留[J]. 食品安全质量检测学报,2016,7(5):1803-1807.
[11]	谢焕龙,王宇,徐振林,等.基于混合抗体的酶联免疫分析方法同时检测孔雀石绿和隐孔雀石绿[J].现代食品科技,2015,31(12):325-330.
[12]	山珊,彭涛,杨万春,等.胶体金免疫层析法定量检测孔雀石绿[J].食品科学,2013,34(16):160-163.
[13]	王春,孟宪双,李文涛,等.介质阻挡放电电离技术及其应用研究进展[J].质谱学报,2018,39(4):500-512.
[14]	闻路红,宁录胜,周振宇,等. 介质阻挡放电离子源在食品安全领域的应用研究[J]. 中国化学会第30届学术年会摘要集-第四十三分会:质谱分析,2016.
[15]	王强,朱鹤云,郑明善,等.DART-MS在公共安全领域的应用研究[J].吉林医药学院学报,2018,39(6):450-454.
[16]	张百乐,高黎红,解迎双,等.实时直接分析-串联质谱法快速筛查火锅底料、牛肉面汤及调味料中罂粟壳生物碱[J]. 色谱,2017,35(7):724-729.
[17]	朱晓月,施捷,张喆,等.实时直接分析质谱法快速筛查葡萄酒中9种甜味剂[J].药物分析杂志,2014,34(10):1817-1822.
[18]	赵鹏,刘云,洪欢欢,等.纳米金辅助介质阻挡放电离子化质谱分析法在兽药饲料快检中的应用[J].分析化学,2018,46(7):1089-1094.
[19]	Kratzer J,Mester Z,Sturgeon R E.Comparison of dielectric barrier discharge,atmospheric pressure radiofrequency-driven glow discharge and direct analysis in real time sources for ambient mass spectrometry of acetaminophen[J].Spectrochimica Acta Part B:Atomic Spectroscopy,2011,66(8):594-603.
[20]	中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 19857-2005水产品中孔雀石绿和结晶紫残留量的测定[S].北京:中国标准出版社,2005.
[21]	Zhang N,Lu M L,Duan X K,et al. In situ calibration of direct analysis in real time-mass spectrometry for direct quantification:Urine excretion rate index creatinine as an example[J].Talanta,2019,201:134-142.
[22]	Mirabelli M F,Wolf J C,Zenobi R.Atmospheric pressure soft ionization for gas chromatography with dielectric barrier discharge ionization-mass spectrometry(GC-DBDI-MS)[J].The Analyst,2017,142(11):1909-1915.

Supplements (0)

Cited By

Cited by

Periodical cited type(2)

1.	樊晓博，高红芳. 水产品中孔雀石绿污染及检测技术研究进展. 食品安全导刊. 2022(11): 166-171 .
2.	劳哲. 原位电离质谱技术的应用与研究进展. 临床研究. 2022(06): 193-198 .