Characteristics of Wild Cherry Beverage Co-fermented by <i>Hanseniaspora uvarum</i> and <i>Saccharomyces cerevisiae</i>

LI Chanyuan; ZHENG Miaoxin; ZOU Yuting; HE Zihan; XU Bitao; ZHANG Qing; QIN Jia; TIAN Wenqiang

doi:10.13386/j.issn1002-0306.2023050212

Volume 45 Issue 7

Mar. 2024

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Science and Technology of Food Industry > 2024 > 45(7): 93-99. > DOI: 10.13386/j.issn1002-0306.2023050212

LI Chanyuan, ZHENG Miaoxin, ZOU Yuting, et al. Characteristics of Wild Cherry Beverage Co-fermented by Hanseniaspora uvarum and Saccharomyces cerevisiae[J]. Science and Technology of Food Industry, 2024, 45(7): 93−99. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023050212.

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Characteristics of Wild Cherry Beverage Co-fermented by Hanseniaspora uvarum and Saccharomyces cerevisiae

1.
School of Food and Bioengineering, Xihua University, Food Microbiology Key Laboratory of Sichuan Province, Chengdu 610039, China
2.
Xiaojin Bingfeng Wine Co., Ltd., Aba Tibetan and Qiang Autonomous Prefecture 624200, China

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Received Date: May 17, 2023
Available Online: January 27, 2024

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Abstract

Abstract

One strain of Hanseniaspora uvarum YT-35 was screened from fermented sediment of wild cherry. Hanseniaspora uvarum YT-35 and commercial Saccharomyces cerevisiae were used as coculture for manufacture of fermented wild cherry beverage. The dynamics of microbial populations, reducing sugars and ethanol were analyzed at different stages of fermentation using single-strain fermentation with 2 strains of bacteria as a control. Meanwhile, the organic acids and volatile aromatic compounds of the fermented beverages were detected by high-performance liquid chromatography (HPLC) and headspace solid-phase microextraction/gas chromatography-mass spectrometry (HS-SPME/GC-MS). The results showed that H. uvarum YT-35 dominated in the pre-fermentation stage of co-culture. Compared with single fermentation with S. cerevisiae, the coculture fermentation resulted in lower ethanol content (3.51 g/L). Notably, HPLC results revealed that coculture fermented beverage reduced the yield of citric, malic and quinic acids and increased the yield of glacial acetic acid. HS-SPME/GC-MS results revealed that coculture fermented beverage produced more volatile compounds of esters, such as ethyl caproate, methyl benzoate and isoamyl octanoate and showed enhanced contents of ethyl laurate, ethyl octanoate, phenyl ethyl alcohol, benzyl alcohol, octanoic acid and lauric acid. Meanwhile, clustering analysis revealed that coculture fermentation were correlated with the greatest number of volatile aroma compounds in the fermented wild cherry beverage. This study provides scientific basis and theoretical guidance for the research of coculture strains with different metabolic potential in improving the quality of fruit juice fermented beverage.
- Hanseniaspora uvarum,
- Saccharomyces cerevisiae,
- coculture,
- wild cherry fermented beverage,
- volatile compounds

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References

[1]	DIENDER M, OLM I P, SOUSA D Z. Synthetic co-cultures:Novel avenues for bio-based processes[J]. Current Opinion in Biotechnology,2021,67(4):72−79.
[2]	XIANG W L, ZAHNG N D, LU Y, et al. Effect of Weissella cibaria co-inoculation on the quality of Sichuan Pickle fermented by Lactobacillus plantarum[J]. LWT-Food Science and Technology,2019,121(2):108975.
[3]	ZHANG Q, SUN Q, TAN X, et al. Characterization of γ-aminobutyric acid (GABA)-producing Saccharomyces cerevisiae and coculture with Lactobacillus plantarum for mulberry beverage brewing[J]. Journal of Bioscience and Bioengineering,2020,129(4):447−453. doi: 10.1016/j.jbiosc.2019.10.001
[4]	易鑫, 谈安群, 欧阳祝, 等. 植物乳杆菌混菌发酵对梁平柚果酒理化性质及风味影响[J]. 食品与发酵工业,2021,47(11):180−187. [YI X, TAN A Q, OUYANG Z, et al. Effects of Lactobacillus plantarum mixed fermentation on the physicochemical properties and flavor of Liangping pomelo wine[J]. Food and Fermentation Industries,2021,47(11):180−187.] YI X, TAN A Q, OUYANG Z, et al. Effects of Lactobacillus plantarum mixed fermentation on the physicochemical properties and flavor of Liangping pomelo wine[J]. Food and Fermentation Industries, 2021, 47（11）: 180−187.
[5]	何荣荣, 彭婧, 孙悦. 接种发酵和自然发酵中酿酒酵母菌株多样性比较[J]. 微生物学报,2021,61(5):1211−1221. [HE R R, PENG J, SUN Y. Yeasts diversity of inoculated and spontaneous fermentations[J]. Acta Microbiologica Sinica,2021,61(5):1211−1221.] HE R R, PENG J, SUN Y. Yeasts diversity of inoculated and spontaneous fermentations[J]. Acta Microbiologica Sinica, 2021, 61（5）: 1211−1221.
[6]	HU L, LIU R, WANG X, et al. The sensory quality improvement of citrus wine through co-fermentations with selected non -Saccharomyces yeast strains and Saccharomyces cerevisiae[J]. Microorganisms,2020,8(3):323−324. doi: 10.3390/microorganisms8030323
[7]	GE Q, GUO C, ZHANG J, et al. Effects of simultaneous co-fermentation of five indigenous non -Saccharomyces strains with S. cerevisiae on vidal icewine aroma quality[J]. Foods,2021,10(7):1452−1455. doi: 10.3390/foods10071452
[8]	LI N, WANG L, YIN J, et al. Adjustment of impact odorants in Hutai-8 rose wine by co-fermentation of Pichia fermentans and Saccharomyces cerevisiae[J]. Food Research International,2022,153(3):1−11.
[9]	战吉宬, 曹梦竹, 游义琳, 等. 非酿酒酵母在葡萄酒酿造中的应用[J]. 中国农业科学,2020,53(19):4057−4069. [ZHAN J C, CAO M Z, YOU Y L, et al. Research advance on the application of non- Saccharomyces in winemaking[J]. Scientia Agricultura Sinica,2020,53(19):4057−4069.] doi: 10.3864/j.issn.0578-1752.2020.19.018 ZHAN J C, CAO M Z, YOU Y L, et al. Research advance on the application of non-Saccharomyces in winemaking[J]. Scientia Agricultura Sinica, 2020, 53（19）: 4057−4069. doi: 10.3864/j.issn.0578-1752.2020.19.018
[10]	王星晨, 胡凯, 陶永胜. 葡萄汁有孢汉逊酵母和酿酒酵母的混合酒精发酵动力学[J]. 食品科学,2016,37(3):103−108. [WANG X C, HU K, TAO Y S. Kinetics of alcohol fermentation by mixed cultures of Hanseniaspora uvarum and Saccharomyces cerevisiae[J]. Food Science,2016,37(3):103−108.] doi: 10.7506/spkx1002-6630-201603020 WANG X C, HU K, TAO Y S. Kinetics of alcohol fermentation by mixed cultures of Hanseniaspora uvarum and Saccharomyces cerevisiae[J]. Food Science, 2016, 37（3）: 103−108. doi: 10.7506/spkx1002-6630-201603020
[11]	HUANG J, WANG Y, REN Y, et al. Effect of inoculation method on the quality and nutritional characteristics of low-alcohol kiwi wine[J]. LWT-Food Science and Technology,2022,156(2):113049.
[12]	MANCIC S, STOJANOVIC S S, DANILOVIC B, et al. Oenological characterization of native Hanseniaspora uvarum Strains[J]. Fermentation-basel,2022,8(3):92. doi: 10.3390/fermentation8030092
[13]	剧柠, 胡婕, 赵梅梅, 等. 葡萄汁有孢汉逊酵母与酿酒酵母混种对发酵枸杞果酒的影响[J]. 食品工业科技,2019,40(6):106−113. [JU N, HU J ZHAO M M, et al. Effects of mixed cultures with Hanseniaspora uvarum and Saccharomyces cerevisiae on fermented wolfberry wine[J]. Science and Technology of Food Industry,2019,40(6):106−113.] JU N, HU J ZHAO M M, et al. Effects of mixed cultures with Hanseniaspora uvarum and Saccharomyces cerevisiae on fermented wolfberry wine[J]. Science and Technology of Food Industry, 2019, 40（6）: 106−113.
[14]	雷丹, 吴敏, 胡琼, 等. 川西高原野樱桃果酒发酵条件优化及其风味成分分析[J]. 食品工业科技,2020,41(24):13−21,30. [LEI D, WU M, HU Q, et al. Optimization of fermentation conditions and volatile components analysis of wild cherry fruit wine of western Sichuan plateau[J]. Science and Technology of Food Industry,2020,41(24):13−21,30.] LEI D, WU M, HU Q, et al. Optimization of fermentation conditions and volatile components analysis of wild cherry fruit wine of western Sichuan plateau[J]. Science and Technology of Food Industry, 2020, 41（24）: 13−21,30.
[15]	梁丽红, 高娉娉, 张馨文, 等. 优选本土非酿酒酵母混合发酵在干红葡萄酒中的应用潜力分析[J]. 食品与发酵工业,2022,48(14):118−124. [LIANG L H, GAO P P, ZHANG X W, et al. Potential application of mixed fermentation of superior indigenous non- Saccharomyces cerevisiae on dry red wine[J]. Food and Fermentation Industries,2022,48(14):118−124.] LIANG L H, GAO P P, ZHANG X W, et al. Potential application of mixed fermentation of superior indigenous non-Saccharomyces cerevisiae on dry red wine[J]. Food and Fermentation Industries, 2022, 48（14）: 118−124.
[16]	卢倩文, 蔡婷, 秦佳, 等. 川西藏区天然野樱桃酒中有机酸含量检测方法的构建[J]. 中国酿造,2016,35(9):154−158. [LU Q W, CAI T, QIN J, et al. Establishment of detection method of organic acids contents in natural wild cherry wine in western Sichuan Tibetan areas[J]. China Brewing,2016,35(9):154−158.] doi: 10.11882/j.issn.0254-5071.2016.09.035 LU Q W, CAI T, QIN J, et al. Establishment of detection method of organic acids contents in natural wild cherry wine in western Sichuan Tibetan areas[J]. China Brewing, 2016, 35（9）: 154−158. doi: 10.11882/j.issn.0254-5071.2016.09.035
[17]	蔡婷, 宋菲菲, 秦佳, 等. 天然野樱桃果酒的酿造及香气成分分析[J]. 中国酿造,2015,34(6):145−149. [CAI T, SONG F F, QIN J, et al. Brewing technology and aroma compounds analysis of natural wild cherry wine[J]. China Brewing,2015,34(6):145−149.] CAI T, SONG F F, QIN J, et al. Brewing technology and aroma compounds analysis of natural wild cherry wine[J]. China Brewing, 2015, 34（6）: 145−149.
[18]	王倩倩, 覃杰, 马得草, 等. 优选发酵毕赤酵母与酿酒酵母混合发酵增香酿造爱格丽干白葡萄酒[J]. 中国农业科学,2018,51(11):2178−2192. [WANG Q Q, QIN J, MA D C, et al. Aroma enhancement of ecolly dry white wine by co-inoculation of selected Pichia fermentans and Saccharomyces cerevisiae[J]. Scientia Agricultura Sinica,2018,51(11):2178−2192.] doi: 10.3864/j.issn.0578-1752.2018.11.015 WANG Q Q, QIN J, MA D C, et al. Aroma enhancement of ecolly dry white wine by co-inoculation of selected Pichia fermentans and Saccharomyces cerevisiae[J]. Scientia Agricultura Sinica, 2018, 51（11）: 2178−2192. doi: 10.3864/j.issn.0578-1752.2018.11.015
[19]	BMA B, MPA B, MPRA B, et al. Use of Hanseniaspora guilliermondii and Hanseniaspora opuntiae to enhance the aromatic profile of beer in mixed-culture fermentation with Saccharomyces cerevisiae[J]. Food Microbiology,2020,95(3):103678.
[20]	BANDIC L M, ZULJ M M, FRUK, G, et al. The profile of organic acids and polyphenols in apple wines fermented with different yeast strains[J]. Journal of Food Science and Technology-mysore,2019,56(2):599−606. doi: 10.1007/s13197-018-3514-2
[21]	刘丛竹. 混菌酿造复合果酒的工艺优化及其主要成分变化规律的研究[D]. 广州:华南理工大学, 2019. [LIU C Z. Study on process optimization and variation of main components of mixed fruit wine produced by mixed bacteria[D]. Guangzhou:South China University of Technology, 2019.] LIU C Z. Study on process optimization and variation of main components of mixed fruit wine produced by mixed bacteria[D]. Guangzhou: South China University of Technology, 2019.
[22]	李宏才. 酵母菌和乳酸菌顺序发酵脱醇苹果汁的制备及降脂机制研究[D]. 杨凌:西北农林科技大学, 2022. [LI H C. Preparation and lipid-lowering mechanism of dealcoholized apple juice fermented sequentially by yeast and lactic acid bacteria[D]. Yangling:Northwest A&F University, 2022.] LI H C. Preparation and lipid-lowering mechanism of dealcoholized apple juice fermented sequentially by yeast and lactic acid bacteria[D]. Yangling: Northwest A&F University, 2022.
[23]	王金玲, 晏雨辰, 李巧月, 等. 生物降解柠檬酸及其影响因素的研究进展[J]. 现代食品科技,2022,38(2):347−357,312. [WANG J L, YAN Y C, LI Q Y, et al. Research progress of biodegradable citric acid and its influencing factors[J]. Modern Food Science and Technology,2022,38(2):347−357,312.] WANG J L, YAN Y C, LI Q Y, et al. Research progress of biodegradable citric acid and its influencing factors[J]. Modern Food Science and Technology, 2022, 38（2）: 347−357,312.
[24]	FURDIKOVA K, MAKYSOVA K, SPANIK I. Effect of indigenous S. cerevisiae strains on higher alcohols, volatile acids, and esters in wine[J]. Czech Journal of Food Sciences,2017,35(2):131−142. doi: 10.17221/79/2016-CJFS
[25]	张文文, 翁佩芳, 吴祖芳. 东方伊萨酵母和酿酒酵母混合发酵杨梅酒的发酵效率及风味特征分析[J]. 食品科学,2019,40(18):144−151. [ZHANG W W, WENG P F, WU Z F. Fermentation efficiency and flavor characteristics of bayberry wine with mixed starter culture of Issatchenkio orientalis and Saccharomyces cerevisiae[J]. Food Science,2019,40(18):144−151.] doi: 10.7506/spkx1002-6630-20180831-375 ZHANG W W, WENG P F, WU Z F. Fermentation efficiency and flavor characteristics of bayberry wine with mixed starter culture of Issatchenkio orientalis and Saccharomyces cerevisiae[J]. Food Science, 2019, 40（18）: 144−151. doi: 10.7506/spkx1002-6630-20180831-375
[26]	CATARINA B, ARLETE M F, PATRICIA L, et al. Genomic expression program of Saccharomyces cerevisiae along a mixed-culture wine fermentation with Hanseniaspora guilliermondii[J]. Microbial Cell Factories,2015(14):124.
[27]	HU K, JIN G J, XU Y H, et al. Wine aroma response to different participation of selected Hanseniaspora uvarum in mixed fermentation with Saccharomyces cerevisiae[J]. Food Research International,2018,108(3):119−127.
[28]	周轩. 浓香型白酒基酒挥发性成分分析及等级识别研究[D]. 镇江:江苏大学, 2019. [ZHOU X. Analysis of volatile compounds of strong-flavor base liquor and study on grade recognition[D]. Zhenjiang:Jiangsu University, 2019.] ZHOU X. Analysis of volatile compounds of strong-flavor base liquor and study on grade recognition[D]. Zhenjiang: Jiangsu University, 2019.
[29]	ZHANG T, FANG K, NI H, et al. Aroma enhancement of instant green tea infusion using β-glucosidase and β-xylosidase[J]. Food Chemistry,2020,315(6):126287.