<sup>1</sup>H-NMR Fingerprint of Hengshan <i>Astragalus membranaceus</i>

ZHANG Hongchi; LIU Rui; BA Defang; LI Hui; YANG Yang; ZHOU Feng

doi:10.13386/j.issn1002-0306.2021030120

Volume 43 Issue 1

Dec. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(1): 47-55. > DOI: 10.13386/j.issn1002-0306.2021030120

ZHANG Hongchi, LIU Rui, BA Defang, et al. ¹H-NMR Fingerprint of Hengshan Astragalus membranaceus[J]. Science and Technology of Food Industry, 2022, 43(1): 47−55. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021030120.

Citation:

PDF (3332 KB)

¹H-NMR Fingerprint of Hengshan Astragalus membranaceus

School of Agriculture and Life Sciences, Shanxi Datong University, Applied Biotechnology Institute, Shanxi Datong University, Datong 037009, China

More Information

Received Date: March 09, 2021
Available Online: November 05, 2021

Graphical Abstract

Abstract

Abstract

Objective: To establish a hydrogen nuclear magnetic resonance (¹H-NMR) fingerprint of Hengshan Astragalus membranaceus, and provide a basis for its quality control. Method: The chemical components were extracted by ultrasonic assisted enzymatic method. Using deuterated chloroform as the extraction solvent, a standard pre-saturated pulse sequence was used to suppress water peaks at 25 ℃ to establish ¹H-NMR fingerprints of 10 batches of A. membranaceus from different sources. The signal attribution was assigned by the qualitative standard addition test, database comparison and related literature comparison. The correlation coefficient method was used to calculate the similarity of ¹H-NMR fingerprints, and the repeatability, precision and stability were investigated. Principal component analysis based on SIMCA was used to evaluate the quality of medicinal materials. Results: 24 compounds were detected and analyzed from Hengshan A. membranaceus, including triterpene saponins, isoflavones, amino acids, organic acids, sugars and so on. The similarities of fingerprints were higher than 0.9 with an average of 0.975. Compared with Mongolian Astragalus membranaceus and Gansu Astragalus membranaceus, the content of isoflavones in Hengshan Astragalus membranaceus was higher than both. PCA results showed that the cumulative contribution rate of the first three principal components was 89.8%, which was basically consistent with the similarity evaluation results. Conclusion: The established ¹H-NMR method had the advantages of quickness and simplicity, which could provide reference for the quality control of Hengshan A. membranaceus.
- Hengshan Astragalus membranaceus,
- hydrogen nuclear magnetic resonance,
- isoflavones,
- saponins,
- fingerprint,
- principal component analysis

FullText(HTML)

References (32)

References

[1]	卞云云, 管佳, 毕志明, 等. 蒙古黄芪的化学成分研究[J]. 中国药学杂志,2006,41(16):1217−1221. [BIAN Y Y, GUAN J, BI Z M, et al. Studies on chemical constituents of Astragalus membranaceus (Fisch. ) Bge. var. mongholicus (Bge. ) Hsiao[J]. Chinese Pharmaceutical Journal,2006,41(16):1217−1221. doi: 10.3321/j.issn:1001-2494.2006.16.006
[2]	LIU R, ZHANG H C, LI H, et al. Obtaining diverse metabolic profiles from endophytic Aspergillus fumigates in Astragalus membranaceus using the one strain-many compounds method[J]. Chemistry of Natural Compounds,2021,57:194−196. doi: 10.1007/s10600-021-03317-x
[3]	ZHANG H C, LIU R, ZHOU F. Chaetoglobosins in endophytic Chaetomium sp. from Astragalus membranaceus[J]. Chemistry of Natural Compounds,2021,57:401−403. doi: 10.1007/s10600-021-03374-2
[4]	田华, 邓雁如, 周坤, 等. 蒙古黄芪的化学成分研究[J]. 中国实验方剂学杂志,2016,22(7):70−73. [TIAN H, DENG Y R, ZHOU K, et al. Chemical constituents of Astragalus membranaceus var. mongholicus[J]. Chinese Journal of Experimental Traditional Medical Formulae,2016,22(7):70−73.
[5]	谢静, 龚易昕悦, 丁立生, 等. 黄芪及其活性成分对脓毒症临床和药理作用的研究进展[J]. 中草药,2021,52(8):2502−2510. [XIE J, GONG Y X Y, DING L S, et al. Progress in clinical and pharmacological studies of Astragali Radix and its active components against sepsis[J]. Chinese Traditional and Herbal Drugs,2021,52(8):2502−2510.
[6]	孔祥琳, 吕琴, 李运伦, 等. 黄芪甲苷对心脑血管疾病的现代药理作用研究进展[J]. 中国实验方剂学杂志,2021,27(2):218−223. [KONG X L, LV Q, LI Y L, et al. Advances in research on pharmacological effect of astragaloside Ⅳ on cardiovascular and cerebrovascular diseases[J]. Chinese Journal of Experimental Traditional Medical Formulae,2021,27(2):218−223.
[7]	易伦朝, 吴海, 梁逸曾. 色谱指纹图谱与中药质量控制[J]. 色谱,2008,26(2):166−171. [YI L Z, WU H, LIANG Y Z. Chromatographic fingerprint and quality control of traditional Chinese medicines[J]. Chinese Journal of Chromatography,2008,26(2):166−171. doi: 10.3321/j.issn:1000-8713.2008.02.007
[8]	姚静, 孙欣光, 董蓉, 等. HPLC-CAD一测多评法同时测定黄芪中6种成分含量[J]. 药学学报,2021,56(2):557−564. [YAO J, SUN X G, DONG R, et al. Simultaneous quantitative analyses of six components in Astragalus membranaceus based on HPLC-CAD and quantitative analysis of multi-components with a single-marker[J]. Acta Pharmaceutica Sinica,2021,56(2):557−564.
[9]	徐翠珊, 陈俭双, 张舒彦, 等. 复方麝香黄芪滴丸HPLC指纹图谱的建立及多成分含量测定[J]. 中国新药杂志,2020,29(16):1894−1901. [XU C X, CHEN J S, ZHANG S Y, et al. Establishment of HPLC fingerprint and determination of multiple components in Compound Shexiang Huangqi Dropping Pills[J]. Chinese Journal of New Drugs,2020,29(16):1894−1901. doi: 10.3969/j.issn.1003-3734.2020.16.017
[10]	LI A P, LI Z Y, JIA J P, et al. Chemical comparison of coat and kernel of mung bean by nuclear magnetic resonance-based metabolic fingerprinting approach[J]. Spectroscopy Letters,2016,49(3):217−224. doi: 10.1080/00387010.2015.1133648
[11]	LI Z Y, ZHANG Z Z, DU G H, et al. Comparative analysis of Danggui and European Danggui using nuclear magnetic resonance-based metabolic fingerprinting[J]. Journal of Pharmaceutical and Biomedical Analysis,2015,103:44−51. doi: 10.1016/j.jpba.2014.10.028
[12]	范刚, 罗尚华, 李艳, 等. 基于¹H-NMR 代谢组学技术的中药质量评价[J]. 世界科学技术-中医药现代化,2013,15(9):1862−1870. [FAN G, LUO S H, LI Y, et al. Quality evaluation of traditional Chinese medicine by H-NMR-based metabolomics[J]. Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology,2013,15(9):1862−1870.
[13]	LIU Y, FAN G, ZHANG J, et al. Metabolic discrimination of sea buckthorn from different Hippophaë species by ¹H-NMR based metabolomics[J]. Scientific Reports,2017,7(1):1585−1595. doi: 10.1038/s41598-017-01722-3
[14]	FAN G, ZHANG M Y, ZHOU X D, et al. Quality evaluation and species differentiation of Rhizoma coptidis by using proton nuclear magnetic resonance spectroscopy[J]. Analytica Chimica Acta,2012,747:76−83. doi: 10.1016/j.aca.2012.08.038
[15]	FAN G, LUO W Z, LUO S H, et al. Metabolic discrimination of Swertia mussotii and Swertia chirayita known a “Zangyinchen” in traditional tibetan medicine by ¹H-NMR-based metabolomicsy[J]. Journal of Pharmaceutical and Biomedical Analysis,2014,98:364−370. doi: 10.1016/j.jpba.2014.06.014
[16]	KIM H K, CHOI Y H, VERPOORTE R. NMR-based metabolomic analysis of plant[J]. Nature Protcols,2010,5(3):536−549. doi: 10.1038/nprot.2009.237
[17]	KOOY VDF, MALTESE F, CHOI Y H, et al. Quality control of herbal material and phytopharmaceuticals with MS and NMR based metabolic fingerprinting[J]. Planta Medica,2009,75(7):763−775. doi: 10.1055/s-0029-1185450
[18]	吕经纬, 李春楠, 高晓晨, 等. 基于核磁共振氢谱代谢组学结合分子对接技术筛选补肾壮骨汤促睾酮合成活性成分[J]. 分析化学,2021,49(5):779−789,147−151. [LV J W, LI C N, GAO X C, et al. Screening of active ingredients of Bushen Zhuanggu decction for promoting testosterone synthesis based on ¹H NMR metabolomics combined with molecular dcking technology[J]. Chinese Journal of Analytical Chemistry,2021,49(5):779−789,147−151.
[19]	齐彦爽, 李静, 郭冬, 等. 基于核磁共振代谢组学技术的款冬茎和叶的化学成分比较[J]. 中国药学杂志,2019,54(8):608−613. [QI Y S, LI J, GUO D, et al. Chemical comparison of the stems and leaves of Tussilago farfara L. using NMR-based metabolomics[J]. Chinese Pharmaceutical Journal,2019,54(8):608−613.
[20]	刘文静, 刘瑶, 宋青青, 等. 利用¹H-NMR比较管花肉苁蓉野生品和栽培品的化学成分组[J]. 中国中药杂志,2018,43(17):3506−3512. [LIU W J, LIU Y, SONG Q Q, et al. Chemome comparison between cultivated and wild Cistanche tubulosa using ¹H-NMR spectroscopy[J]. China Journal of Chinese Materia Medica,2018,43(17):3506−3512.
[21]	李玮, 杨红梅, 王浩, 等. 基于¹H-NMR代谢组学初步比较真蜂蜜和掺假蜂蜜差异成分[J]. 食品工业科技,2019,40(7):218−223,227. [LI W, YANG H M, WANG H, et al. Comparison of chemical constituents in honey and adulterated honey based on ¹H-NMR metabonomics[J]. Science and Technology of Food Industry,2019,40(7):218−223,227.
[22]	郭淑娟, 朱晟永, 贾悦, 等. 基于主成分分析微波提取圆头蒿多糖工艺中单糖组分变化[J]. 食品工业科技,2020,41(22):267−274. [GUO S J, ZHU S Y, JIA Y, et al. Changes of monosaccharide components in microwave extraction of Artemisia sphaercephala polysaccharides based on principal component analysis[J]. Science and Technology of Food Industry,2020,41(22):267−274.
[23]	何佳, 黄文康, 马相锋, 等. 基于主成分分析与PLS-DA分析研究浙麦冬道地性与等级评价标准[J]. 中国药学杂志,2021,56(4):285−292. [HE J, HUANG W K, MA X F, et al. Geoherbalism and grand evaluation of Zhejiang Ophiopogon japonicas based on PCA and PLS-DA[J]. Chinese Pharmaceutical Journal,2021,56(4):285−292.
[24]	王青虎, 王秀兰, 奥·乌力吉, 等. 蒙古黄芪化学成分的研究[J]. 中国药学杂志,2014,49(5):357−359. [WANG Q H, WANG X L, AO W L J, et al. Chemical constituents of roots of Astragalus membranaceus(Fisch) Bge. var. mongholicus (Bge) Hsiao[J]. Chinese Pharmaceutical Journal,2014,49(5):357−359.
[25]	孙洁, 张蕾, 张晓拢, 等. 蒙古黄芪的化学成分研究[J]. 现代药物与临床,2013,28(2):138−143. [SUN J, ZHANG L, ZHANG X L, et al. Chemical constituents from Astragalus membranaceus var. mongholicus[J]. Drugs and Clinic,2013,28(2):138−143.
[26]	ALANIYA M D, KAVTARADZE N S, LAVOIE S, et al. Chemical constituents of the aerial part of Astragalus bungeanus[J]. Chemistry of Natural Compounds,2011,46:1001−1003. doi: 10.1007/s10600-011-9811-x
[27]	HE Z Q, FINDLAY J A. Constituents of Astragalus membranaceus[J]. Journal of Natural Products,1991,54(3):810−815. doi: 10.1021/np50075a009
[28]	SAFFARIHA M, AZARNIVAND H, ZARE CHAHOUKI M A, et al. Changes in the essential oil content and composition of Salvia limbata C. A. Mey at different growth stages and altitudes[J]. Biomedical Chromatography,2021:e5127.
[29]	TANG J F, WANG W D, YANG L, et al. Variation in quantity and chemical composition of soil dissolved organic matter in a peri-urban critical zone observatory watershed in Eastern China[J]. Science of The Total Environment,2019,688:622−631. doi: 10.1016/j.scitotenv.2019.06.270
[30]	沈燕金, 张一扬, 李德青, 等. 文山烟叶主要化学成分与海拔经纬度相关性分析[J]. 湖南农业科学,2015(11):29−31. [SHEN Y J, ZHANG Y Y, LI D Q, et al. Correlation analysis between chemical compositions of flue-cured tobacco leaves and altitude, longitude and latitude in Wenshan[J]. Hunan Agricultural Sciences,2015(11):29−31.
[31]	刘瑞. 恒山黄芪内生真菌及其代谢产物研究与应用[M]. 北京: 中国农业出版社, 2020: 1−12. LIU R. Research and application of metabolites of endophytic fungi from Hengshan Aspergillus fumigatus[M]. Beijing: China Agriculture Press, 2020: 1−12.
[32]	胡明勋, 陈安家, 郭宝林, 等. 影响山西恒山野生蒙古黄芪质量的环境因素研究[J]. 中草药,2005,40(5):984−989. [HU M X, CHEN A J, GUO B L, et al. Effects of environmental factors on quality of wild Astragalus membranaceus var. mongholicus grown in Hengshan Mountain, Shanxi Province[J]. Chinese Traditional and Herbal Drugs,2005,40(5):984−989.