Anti-inflammatory Mechanism of Phenolic Compounds from <i>Sargassum fusiforme</i> by LPS-induced Zebrafish Embryo Model

NI Li-ying; ZOU Ya-xue; FU Xiao-ting; DUAN De-lin; XU Jia-chao; GAO Xin

doi:10.13386/j.issn1002-0306.2019.21.046

Volume 40 Issue 21

Oct. 2019

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Science and Technology of Food Industry > 2019 > 40(21): 279-285. > DOI: 10.13386/j.issn1002-0306.2019.21.046

NI Li-ying, ZOU Ya-xue, FU Xiao-ting, DUAN De-lin, XU Jia-chao, GAO Xin. Anti-inflammatory Mechanism of Phenolic Compounds from Sargassum fusiforme by LPS-induced Zebrafish Embryo Model[J]. Science and Technology of Food Industry, 2019, 40(21): 279-285. DOI: 10.13386/j.issn1002-0306.2019.21.046

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Anti-inflammatory Mechanism of Phenolic Compounds from Sargassum fusiforme by LPS-induced Zebrafish Embryo Model

1. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;
2. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

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Received Date: January 14, 2019
Available Online: November 12, 2020

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Abstract

Abstract

The anti-inflammatory activities and mechanisms of phenolic compounds from Sargassum fusiforme(SFPC)were studied in lipopolysaccharides(LPS)-induced zebrafish model. The optimal LPS-induced inflammatory model in zebrafish was established,and the anti-inflammatory activities of SFPC with different concentrations were investigated by determination of yolk sac size,heart-beating rate,NO production rate,cell death rate and reactive oxygen(ROS)production rate. The results showed that SFPC(50,100,200 μg/mL)had a protective effect on inflammation induced by LPS(8 μg/mL)in a dose-dependent manner. The optimal concentration of 200 μg/mL SFPC significantly reduced the heart-beating rate(102.56%,P<0.05)and yolk sac size(109.18%,P<0.05). The rates of NO production,cell death and ROS production decreased from 202.48%,168.45% and 353.44% to 75.62%,101.25% and 128.91%,respectively. In this study,the zebrafish model was used for the first time to reveal the anti-inflammatory activity of SFPC in vivo. This study provides a theoretical basis for the application of SFPC as anti-inflammatory agent in functional food and cosmetics.
- phenolic,
- zebrafish,
- anti-inflammatory,
- fluorescence

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