NI Li-ying, ZOU Ya-xue, FU Xiao-ting, DUAN De-lin, XU Jia-chao, GAO Xin. Anti-inflammatory Mechanism of Phenolic Compounds from Sargassum fusiforme by LPS-induced Zebrafish Embryo Model[J]. Science and Technology of Food Industry, 2019, 40(21): 279-285. DOI: 10.13386/j.issn1002-0306.2019.21.046
Citation:
NI Li-ying, ZOU Ya-xue, FU Xiao-ting, DUAN De-lin, XU Jia-chao, GAO Xin. Anti-inflammatory Mechanism of Phenolic Compounds from Sargassum fusiforme by LPS-induced Zebrafish Embryo Model[J]. Science and Technology of Food Industry, 2019, 40(21): 279-285. DOI: 10.13386/j.issn1002-0306.2019.21.046
NI Li-ying, ZOU Ya-xue, FU Xiao-ting, DUAN De-lin, XU Jia-chao, GAO Xin. Anti-inflammatory Mechanism of Phenolic Compounds from Sargassum fusiforme by LPS-induced Zebrafish Embryo Model[J]. Science and Technology of Food Industry, 2019, 40(21): 279-285. DOI: 10.13386/j.issn1002-0306.2019.21.046
Citation:
NI Li-ying, ZOU Ya-xue, FU Xiao-ting, DUAN De-lin, XU Jia-chao, GAO Xin. Anti-inflammatory Mechanism of Phenolic Compounds from Sargassum fusiforme by LPS-induced Zebrafish Embryo Model[J]. Science and Technology of Food Industry, 2019, 40(21): 279-285. DOI: 10.13386/j.issn1002-0306.2019.21.046
The anti-inflammatory activities and mechanisms of phenolic compounds from Sargassum fusiforme(SFPC)were studied in lipopolysaccharides(LPS)-induced zebrafish model. The optimal LPS-induced inflammatory model in zebrafish was established,and the anti-inflammatory activities of SFPC with different concentrations were investigated by determination of yolk sac size,heart-beating rate,NO production rate,cell death rate and reactive oxygen(ROS)production rate. The results showed that SFPC(50,100,200 μg/mL)had a protective effect on inflammation induced by LPS(8 μg/mL)in a dose-dependent manner. The optimal concentration of 200 μg/mL SFPC significantly reduced the heart-beating rate(102.56%,P<0.05)and yolk sac size(109.18%,P<0.05). The rates of NO production,cell death and ROS production decreased from 202.48%,168.45% and 353.44% to 75.62%,101.25% and 128.91%,respectively. In this study,the zebrafish model was used for the first time to reveal the anti-inflammatory activity of SFPC in vivo. This study provides a theoretical basis for the application of SFPC as anti-inflammatory agent in functional food and cosmetics.
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