金属离子对蛹虫草液体培养产胞外虫草素产量的影响

基金项目:

北京市科学技术研究院“萌芽计划”项目；北京市科学技术研究院“青年骨干计划”项目(201311)；

详细信息

Effect of metal ions on extracellular cordycepin production in submerged cultivation of Cordyceps militaris

摘要: 主要研究不同金属离子对蛹虫草液体培养胞外虫草素产量及菌丝体生物量的影响。将含有Mg2+、Fe2+、Fe3+、Zn2+、Mn2+、Cu2+、Ca2+、Co2+及I-的化合物添加到蛹虫草液体培养基中,选择出有利于胞外虫草素产量及菌丝体生物量增加的金属离子,确定其最佳添加浓度。实验结果表明:添加Mn2+、Ca2+、Mg2+能够显著（p<0.05）提高蛹虫草液体培养胞外虫草素产量及菌丝体生物量,其最适浓度分别为Mn2+0.05 g/L、Ca2+0.6 g/L、Mg2+1.0 g/L。
- 蛹虫草 /
- 液体培养 /
- 胞外虫草素 /
- 金属离子 /
- 菌丝体干重
Abstract: The study was aimed at the effect of different kinds of metal ions on extracellular cordycepin production and biomass by submerged cultivation of Cordyceps militaris. Compounds contained Mg2 +,Fe2 +,Fe3 +,Zn2 +,Mn2 +,Cu2 +,Ca2 +,Co2 +and I-were added to liquid culture medium respectively to select some kinds of metal ions that could facilitate the extracellular cordycepin production and biomass. And on this basis,the optimum concentration was confirmed. The results suggested that Mn2 +,Ca2 +and Mg2 +significantly improved the production of cordycepin and biomass,and the suitable concentrations were 0.05 g/L Mn2+,0.6 g/L Ca2+and1.0 g/L Mg2+respectively.
- Cordyceps militaris /
- submerged cultivation /
- extracellular cordycepin /
- metal ions /
- dry cell weight