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中国精品科技期刊2020
何坤明, 陈兰转, 孙光友, 谭旺桥, 白新鹏. 山茱萸籽粕亚临界水降解动力学及多糖性质研究[J]. 食品工业科技, 2020, 41(23): 37-44. DOI: 10.13386/j.issn1002-0306.2020020199
引用本文: 何坤明, 陈兰转, 孙光友, 谭旺桥, 白新鹏. 山茱萸籽粕亚临界水降解动力学及多糖性质研究[J]. 食品工业科技, 2020, 41(23): 37-44. DOI: 10.13386/j.issn1002-0306.2020020199
HE Kun-ming, CHEN Lan-zhuan, SUN Guang-you, TAN Wang-qiao, BAI Xin-peng. Subcritical Water Degradation Kinetics of Cornus officinalis Seed Meal and Its Polysaccharide Properties[J]. Science and Technology of Food Industry, 2020, 41(23): 37-44. DOI: 10.13386/j.issn1002-0306.2020020199
Citation: HE Kun-ming, CHEN Lan-zhuan, SUN Guang-you, TAN Wang-qiao, BAI Xin-peng. Subcritical Water Degradation Kinetics of Cornus officinalis Seed Meal and Its Polysaccharide Properties[J]. Science and Technology of Food Industry, 2020, 41(23): 37-44. DOI: 10.13386/j.issn1002-0306.2020020199

山茱萸籽粕亚临界水降解动力学及多糖性质研究

Subcritical Water Degradation Kinetics of Cornus officinalis Seed Meal and Its Polysaccharide Properties

  • 摘要: 本实验以山茱萸籽粕为原料,进行山茱萸籽粕亚临界水降解研究,测定不同降解温度、降解时间下液态产物的还原糖含量,采用Saeman模型模拟分析,建立降解动力学方程,得到降解动力学参数,并对降解物进行理化性质的分析。结果表明,Saeman模型能较好反映山茱萸籽粕亚临界水降解的过程,初步降解的反应活化能Ea1为29.47 kJ/mol、还原糖降解活化能Ea2为22.21 kJ/mol、指前因子K10为101.49 min-1、K20为20.28 min-1,在降解温度160 ℃、降解时间20 min条件下,能得到产量较高的还原糖。单糖组成分析表明,山茱萸籽多糖主要由葡萄糖、木糖、半乳糖、半乳糖醛酸组成,其总糖含量高达78.82%;扫描电镜和红外光谱结构分析结果表明,多糖结构呈表面光滑,无规则颗粒状,粒径大小不一的球状结构物,为吡喃型糖苷环骨架;抗氧化活性分析结果表明,山茱萸籽多糖具有较好的自由基清除能力,在质量浓度为1.0 mg/mL时,对DPPH自由基、羟自由基和ABTS自由基清除率分别为85.61%、63.40%、76.20%。本试验结果为山茱萸籽多糖的进一步分离纯化和理化特性、形态结构的分析提供了参考。

     

    Abstract: In this study,Cornus officinalis seed meal was used as raw material to conduct a subcritical water degradation study,and the reducing sugar content of the liquid product was measured at different degradation temperatures and degradation time. The Saeman model was adopted to simulate the analysis,and the degradation kinetics equation was established to obtain the degradation kinetics parameters,and the physicochemical properties of the degraded product were analyzed. The results showed that the Saeman model could better reflect the process of subcritical water degradation of Cornus officinalis seed meal,with the reaction activation energy Ea1 of primary degradation being 29.47 kJ/mol,the reduction sugar activation energy Ea2 being 22.21 kJ/mol,the preexponential factor K10 being 101.49 min-1,and K20 being 20.28 min-1.At the degradation temperature of 160 ℃ and the degradation time of 20 min,the reducing sugar with higher yield could be obtained. The monosaccharide analysis showed that the polysaccharide of Cornus officinalis was mainly composed of glucose,xylose,galactose and galacturonic acid,and its total sugar content was as high as 78.82%.Preliminary structural analysis results by scanning electron microscopy and infrared spectroscopy showed that polysaccharides were spherical structures with smooth surface,irregular granule and different particle sizes,and they were pyranoid glycoside ring skeletons. The results of antioxidant activity analysis showed that the polysaccharides of Cornus officinalis had good free radical scavenging ability. At the mass concentration of 1.0 mg/mL,the scavenging rates of DPPH free radical,hydroxyl free radical and ABTS free radical were 85.61%,63.40% and 76.20%,respectively.The results of this experiment provide reference for the further separation and purification of polysaccharides from cornus officinalis seed and the analysis of their physicochemical characteristics and morphological structure.

     

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