摘要:
以黄皮疣柄牛肝菌Leccinellum crocipodium(Letellier.)Watliag为原料,采用水提醇沉法从黄皮疣柄牛肝菌子实体中提取得到水提粗多糖(LPS),再用碱提醇沉法从菌渣中提取得到碱提粗多糖(LPJ),研究两种黄皮疣柄牛肝菌粗多糖理化性质与抗氧化活性。结果表明:水提粗多糖LPS与碱提粗多糖LPJ得率分别为18.44%±1.30%与5.50%±0.69%。LPS呈海绵状,颜色呈浅咖色;LPJ呈蓬松粉末状,颜色呈黄褐色。LPS多糖含量、糖醛酸含量、硫酸根含量、总酚含量均显著高于LPJ(P<0.05)。LPS与LPJ抗氧化活性随着浓度的增加而逐渐增强。LPS、LPJ浓度为4 mg/mL时,DPPH自由基的清除率(87.55%±0.51%、54.31%±2.72%)、羟基自由基清除率(54.53%±1.61%、46.50%±0.64%)、ABTS阳离子自由基清除率(81.56%±4.43%、68.79%±1.23%)、还原力吸光度值(1.41±0.02、1.16±0.01)均达到最大值,而浓度为3 mg/mL时,超氧阴离子自由基清除率(89.16%±2.42%、85.94%±2.98%)达到最大值。说明LPS与LPJ具有较好的抗氧化能力,且LPS优于LPJ。
Abstract:
With Leccinellum crocipodium (Letellier.) Watliag as raw material, the water-extracted crude polysaccharide (LPS) was extracted from the fruiting body of Leccinellum crocipodium (Letellier.) Watliag by water-extraction and alcohol-precipitation method, and the alkaline-extracted crude polysaccharide (LPJ) was extracted from the fungus residue by alkaline-extraction and alcohol-precipitation method. The physical and chemical properties and antioxidant activity of two crude polysaccharides were studied. The results showed that the yields of water-extracted crude polysaccharide LPS and alkaline-extracted crude polysaccharide LPJ were 18.44%±1.30% and 5.50%±0.69%, respectively. LPS was spongy and light brown;LPJ was fluffy and yellowish brown. The polysaccharide content, uronic acid content, sulfate content, and total phenols content of LPS were all higher than LPJ (P<0.05). The antioxidant activity of LPS and LPJ increased gradually with increasing concentration. When the concentration of LPS and LPJ was 4 mg/mL, the scavenging rate of DPPH radicals (87.55%±0.51%, 54.31%±2.72%), the scavenging rate of hydroxyl radicals (54.53%±1.61%, 46.50%±0.64%), the scavenging rate of ABTS cationic radicals (81.56%±4.43%, 68.79%±1.23%), and reducing power absorbance values (1.41±0.02, 1.16±0.01) all reached the maximum, when the concentration was 3 mg/mL, the scavenging rate of superoxide anion radicals (89.16%±2.42%, 85.94%±2.98%) reached the maximum. It showed that LPS and LPJ had better antioxidant ability, and LPS was better than LPJ.
百度学术
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