Optimization of Extraction Process and Comparison of Total Flavonoids Contents of Different Ages and Strains of Anoectochilus roxburghii
-
摘要: 目的:应用均匀设计法优化金线莲总黄酮提取工艺,并比较不同月龄和不同品系金线莲总黄酮的含量。方法:采用均匀设计法,考察乙醇浓度、液料比、提取时间和提取次数对金线莲总黄酮得率的影响,进行验证并测定不同月龄、不同品系金线莲总黄酮含量。结果:金线莲总黄酮最佳提取工艺为40倍量88%乙醇超声提取3次,每次80 min;验证试验总黄酮得率为1.68%(RSD=0.27%,n=3)。金线莲总黄酮含量与品系和月龄有关,组培金线莲总黄酮平均含量为11.091 mg/g,栽培4月龄和5月龄各品系金线莲总黄酮平均含量分别为14.359和18.730 mg/g,栽培4月龄的金线莲总黄酮含量由高到低为:大圆叶 > 尖叶 > 红霞 > 本地尖叶 > 本地圆叶 > 本地野生,组培金线莲经土壤种植其次级代谢产物总黄酮含量呈逐渐累积状态,在5月龄达到最高值。结论:优化的超声提取工艺合理、稳定、可行,为金线莲总黄酮测定和质量评价提供参考。组培金线莲和栽培金线莲总黄酮含量存在差异,土壤栽培有利于金线莲有效成分的积累,因此组培金线莲经土壤栽培是必要的。Abstract: Objective:To optimize the extraction technology of total flavonoids from Anoectochilus roxburghii,the uniform design method was used and the total flavonoids contents of A. roxburghii of different months and various Anoectochilus spp were compared. Method:Uniform design method was applied to investigate the factors such as ethanol concentration,liquid material ratio,extraction time and extraction rate on total flavonoids extraction of A. roxburghii. The optimized extraction method from uniform design method was then validated by determined the flavonoid contents of different months A. roxburghii and other Anoectochilus spp. Results:Optimized extraction technology was found to be 40 volumes of 88% ethanol,80 min ultrasonic extraction,and 3 times of repetition. The total flavonoids extraction rate in verification test was 1.68%(RSD=0.27%,n=3). The total flavonoid contents of A. roxburghii was correlated to its strain and month of ages. The average contents of total flavonoids in A. roxburghii tissue cultured was 11.091 mg/g,The average contents of total flavonoids in 4 and 5 cultivated month age of cultivated A. roxburghii were 14.359 and 18.730 mg/g,respectively. The amount of total flavonoids obtained from the 4 cultivated month age of A. roxburghii arranged in decreasing order as Da Yuanye > Jian Ye > Hong Xia > Local Jianye > Local Yuanye > Local Wild. The finding also suggested that the secondary metabolites contents especially flavonoid compound in cultivated A. roxburghii was believed to be increased gradually,and reached the highest value at the age of 5 month. Conclusion:The optimized ultrasonic extraction process was reasonable,stable,and feasible,would provide a reference for the determination and quality evaluation of total flavonoids in A. roxburghii. There had differences between tissue culture and cultivated A. roxburghii in the contents of total flavonoids. The soil cultivation was beneficial to the accumulation of the active constituents of A. acuminata. Therefore,it was necessary to cultivate the golden lotus seedlings through soil cultivation.
-
Keywords:
- Anoectochilus roxburghii /
- total flavonoids /
- uniform design /
- different month ages
-
期刊类型引用(17)
1. 张闻婷，梅瑜，王继华. 珍稀药用植物金线莲研究现状与展望. 中国农学通报. 2024(05): 16-26 . 
百度学术
2. Liu Xiaochun，Chen Laicheng，Yang Zhanhong，Zou Jie. Advances in the Study of Anoectochilus Roxburghii Extracts and Prospects for Cosmetic Application Potentials. China Detergent & Cosmetics. 2024(01): 45-53 . 必应学术
3. 陈志春，李成伟，陈莹，王英豪，陈桂芬. 基于等高线生成算法的多花黄精中多糖、皂苷提取工艺优化研究. 福建中医药. 2024(02): 57-64 . 百度学术
4. 穆晓琨，卢素华，严丹峰，冯美莹. 组培和大棚培育下肇庆地区金线莲总黄酮含量分析. 现代畜牧科技. 2024(04): 27-30 . 百度学术
5. 向羽，周成，黄楚天，何辉，柳洪入. 结构优化对不同叶位烟叶品质及挥发性物质含量的影响. 安徽农业科学. 2024(10): 15-20 . 百度学术
6. 向羽，黄闫江，周成，黄楚天，何辉，柳洪入. 增施农家肥对不同区域烟叶香气品质的影响. 安徽农业科学. 2024(13): 121-126 . 百度学术
7. 邱道寿，罗永坚，郑武林，李莉. 棚栽与瓶栽金线莲的广靶代谢组学比较. 亚热带农业研究. 2024(02): 73-82 . 百度学术
8. 黎霞，何佳，王建栋，申屠文姬，王红珍. 反相高效液相色谱法检测金线莲中槲皮素、山奈酚含量. 浙江农业科学. 2023(02): 446-449 . 百度学术
9. 何辉，柳洪入，乔勇进，张怡，王春芳，陈冰洁，刘晨霞，王晓，邬松恒. 不同品系及生长时间金线莲活性成分及挥发性物质比较分析. 福建农业学报. 2023(03): 271-280 . 百度学术
10. 黄琼，谢向机. 金线莲多糖提取工艺优化及其体外抗氧化活性研究. 佛山科学技术学院学报(自然科学版). 2023(03): 36-44 . 百度学术
11. 吴梅，高一舟，方莉，周小军，陈德权，汪芳，许勇泉，徐金晶，丁莫芷，钭凌娟. 不同加工方式对金线莲茶品质的影响. 特产研究. 2023(04): 98-102+111 . 百度学术
12. 刘晓纯，陈来成，杨占红，邹洁，何秋星. 金线莲提取物研究现状及其在化妆品中的应用前景. 日用化学品科学. 2023(11): 42-46 . 百度学术
13. 甘勇辉，王水琦. 金线莲施硒的吸收与积累规律. 中国土壤与肥料. 2023(08): 214-221 . 百度学术
14. 徐嘉鸿，刘美美，戚滇杰，俞杰，许晓路，ZHYLKO Viachaslau. 金花茶花总黄酮双水相提取工艺优化及其抗氧化活性分析. 食品工业科技. 2022(03): 155-161 . 本站查看
15. 陈莹，王文义，谌赛男，黄锦芳，张雪，吴水生，孙志蓉，陈菁瑛. 不同品系及生长期金线莲的金线莲苷含量变化研究. 中国现代中药. 2021(08): 1423-1429 . 百度学术
16. 陈健敏，刘思弯，冉梦楠，王美霞，刘欣莹. 金线莲醇提物和水提物生物活性研究. 河南大学学报(医学版). 2021(05): 322-328 . 百度学术
17. 黄哲，高云，党友超，杨烨，李蒙禹，王世清. 黔产金线莲总黄酮含量测定研究. 贵州中医药大学学报. 2020(06): 23-27 . 百度学术
其他类型引用(4)
计量
- 文章访问数: 351
- HTML全文浏览量: 72
- PDF下载量: 6
- 被引次数: 21
下载:
