摘要:
目的:应用均匀设计法优化金线莲总黄酮提取工艺,并比较不同月龄和不同品系金线莲总黄酮的含量。方法:采用均匀设计法,考察乙醇浓度、液料比、提取时间和提取次数对金线莲总黄酮得率的影响,进行验证并测定不同月龄、不同品系金线莲总黄酮含量。结果:金线莲总黄酮最佳提取工艺为40倍量88%乙醇超声提取3次,每次80 min;验证试验总黄酮得率为1.68%(RSD=0.27%,n=3)。金线莲总黄酮含量与品系和月龄有关,组培金线莲总黄酮平均含量为11.091 mg/g,栽培4月龄和5月龄各品系金线莲总黄酮平均含量分别为14.359和18.730 mg/g,栽培4月龄的金线莲总黄酮含量由高到低为:大圆叶 > 尖叶 > 红霞 > 本地尖叶 > 本地圆叶 > 本地野生,组培金线莲经土壤种植其次级代谢产物总黄酮含量呈逐渐累积状态,在5月龄达到最高值。结论:优化的超声提取工艺合理、稳定、可行,为金线莲总黄酮测定和质量评价提供参考。组培金线莲和栽培金线莲总黄酮含量存在差异,土壤栽培有利于金线莲有效成分的积累,因此组培金线莲经土壤栽培是必要的。
关键词:
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金线莲
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总黄酮
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均匀设计
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不同月龄
Abstract:
Objective:To optimize the extraction technology of total flavonoids from Anoectochilus roxburghii,the uniform design method was used and the total flavonoids contents of A. roxburghii of different months and various Anoectochilus spp were compared. Method:Uniform design method was applied to investigate the factors such as ethanol concentration,liquid material ratio,extraction time and extraction rate on total flavonoids extraction of A. roxburghii. The optimized extraction method from uniform design method was then validated by determined the flavonoid contents of different months A. roxburghii and other Anoectochilus spp. Results:Optimized extraction technology was found to be 40 volumes of 88% ethanol,80 min ultrasonic extraction,and 3 times of repetition. The total flavonoids extraction rate in verification test was 1.68%(RSD=0.27%,n=3). The total flavonoid contents of A. roxburghii was correlated to its strain and month of ages. The average contents of total flavonoids in A. roxburghii tissue cultured was 11.091 mg/g,The average contents of total flavonoids in 4 and 5 cultivated month age of cultivated A. roxburghii were 14.359 and 18.730 mg/g,respectively. The amount of total flavonoids obtained from the 4 cultivated month age of A. roxburghii arranged in decreasing order as Da Yuanye > Jian Ye > Hong Xia > Local Jianye > Local Yuanye > Local Wild. The finding also suggested that the secondary metabolites contents especially flavonoid compound in cultivated A. roxburghii was believed to be increased gradually,and reached the highest value at the age of 5 month. Conclusion:The optimized ultrasonic extraction process was reasonable,stable,and feasible,would provide a reference for the determination and quality evaluation of total flavonoids in A. roxburghii. There had differences between tissue culture and cultivated A. roxburghii in the contents of total flavonoids. The soil cultivation was beneficial to the accumulation of the active constituents of A. acuminata. Therefore,it was necessary to cultivate the golden lotus seedlings through soil cultivation.
百度学术
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