Optimization of Extracting Process of Dioscorea opposite Thunb Polysaccharide by Supercritical CO2 Fluid Extraction Based on Response Surface Method
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摘要: 以淮山为试验原料,采用超临界CO2流体萃取淮山多糖。在单因素实验基础上,通过响应面法研究了萃取温度、压力、时间、夹带剂乙醇浓度4个因素对淮山多糖萃取得率的影响,优化了萃取工艺。结果表明,超临界CO2流体萃取淮山多糖的最佳工艺条件为:萃取温度53 ℃、萃取压力45 MPa、萃取时间2.5 h、夹带剂乙醇浓度68%,该条件下进行三次重复试验,淮山多糖萃取得率为0.2807%±0.0045%,与预测值0.287%接近。以上结果表明,响应面法优化淮山多糖萃取工艺后,可有效提高其多糖萃取得率。Abstract: The supercritical CO2 extraction process with the ethanol aqueous solution was used to extract the polysaccharide from Dioscorea opposite Thunb,and the response surface methodology(RSM)was used to optimize the process parameters. On the basis of single-factor experiments,extraction time,pressure,temperature and the ethanol content were selected as four independent variables. An experimental design was carried out involving four variables at three levels. The results showed that the optimum process conditions for extraction of Dioscorea opposite Thunb polysaccharide by supercritical CO2 fluid were:the extraction temperature of 53 ℃,extraction pressure of 45 MPa,extraction time of 2.5 h,the dosage of ethanol content 68%,the estimated and observed values of maximal yield of polysaccharide was 0.287% and 0.2807%±0.0045%,respectively. The results showed that the extraction yield of polysaccharides from Dioscorea opposite Thunb could be effectively improved by optimizing the extraction process with response surface methodology.
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Keywords:
- response surface methodology /
- SFE-CO2 /
- Dioscorea opposite Thunb /
- polysaccharides
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