摘要:
目的:研究柳叶蜡梅醇提物两种极性组分对无水乙醇致急性胃黏膜损伤大鼠的保护作用。方法:采用无水乙醇为诱导剂构建胃黏膜损伤小鼠模型。将大鼠分为空白对照组、模型组、阳性瑞巴派特组、乙酸乙酯萃取(Ethyl acetate fraction,EF)剂量组(400、200、100、50 mg·kg-1·bw)、石油醚萃取(Petroleum ether fraction,PF)剂量组(400、200、100、50 mg·kg-1·bw),连续灌胃14 d。研究柳叶蜡梅醇提物的两种极性组分对胃黏膜抗氧化、抗炎水平及酸性粘液蛋白的分泌情况,并观察胃黏膜的组织病理学变化,酸性粘液蛋白水平和溃疡抑制率的变化。结果:EF和PF各剂量组均能改善胃黏膜的损伤情况,浓度高的效果好于浓度低的效果。与模型组相比,EF高剂量组(400 mg/kg)能极显著提高胃组织中超氧化物歧化酶(Superoxide Dismutase,SOD)(P<0.01)和过氧化氢酶(Catalase,CAT)(P<0.05)的活性,降低前列腺素E2(Prostaglandin E2,PGE2)(P<0.01)、丙二醛(Malonaldehyde,MDA(P<0.01)和一氧化氮(Nitric Oxide,NO)(P<0.05)的含量;PF高剂量组(400 mg/kg)的PGE2含量极显著下降(P<0.01),胃组织中SOD(P<0.05)、CAT酶活力显著提高(P<0.01),MDA、NO含量无显著影响。从胃部溃疡情况和切片的分析来看,EF和PF组分均能提高溃疡抑制率和胃黏膜酸性粘液蛋白面积并减少无水乙醇导致的溃疡面积,降低胃损伤程度。结论:柳叶蜡梅醇提物的EF和PF组分对无水乙醇致大鼠急性胃黏膜损伤具有保护作用,机制可能与其抗氧化效果,抗炎及增强胃黏膜保护因子有关。
Abstract:
Objective:Studying the effect of two polar components of Chimonanthus salicifolius aleoholic extracts on mice with anhydrous ethanol-induced acute gastric mucosal injury. Methods:A mice model with acute alcoholic gastric injury was established by intragastric administration of ethanol. The mice were divided into control group,model group,rebamipide control group,ethyl acetate fraction group(EF,400,200,100,50 mg·kg-1·bw) and petroleum ether fraction group(PF,400,200,100,50 mg·kg-1·bw),and mice were treated continually for 14 d. In this study,the antioxidant and anti-inflammatory effects of EF and PF on gastric mucosa were determined. The histopathological changes of gastric mucosa and acidic mucus proteins were observed by H&E and AB-PAS staining. Results:The two polar components reduced the damage of gastric mucosa,and the effect of high concentration was better than low concentration. EF(400 mg/kg) significantly increased the level of SOD(P<0.01) and CAT(P<0.05),and reduced the contents of PGE2(P<0.01),MDA(P<0.01) and NO(P<0.05) compared with the model group. Moreover,PF(400 mg/mL) significantly decreased the contents of PGE2(P<0.01) and increased the level of SOD(P<0.05) and CAT(P<0.01) in gastric tissue,while the contents of MDA and NO did not alter. From the analysis of gastric ulcer conditions and sections,EF and PF inhibited the gastric ulcer and reduced the area of ulcer caused by alcohol as well as the degree of gastric injury. Conclusion:The alcohol extracts from Chimonanthus salicifolius had protective effects on ethanol-induced mice with gastric injury,and this mechanism would be related to the antioxidant effect,anti-inflammatory effect and gastric mucosal protective factor.
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