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中国精品科技期刊2020

超高效液相色谱-荧光检测法测定水果中3种萘衍生物

仲伶俐, 李曦, 郭灵安, 李华仙, 雷欣宇, 付成平, 赵珊

仲伶俐, 李曦, 郭灵安, 李华仙, 雷欣宇, 付成平, 赵珊. 超高效液相色谱-荧光检测法测定水果中3种萘衍生物[J]. 食品工业科技, 2018, 39(14): 200-204. DOI: 10.13386/j.issn1002-0306.2018.14.037
引用本文: 仲伶俐, 李曦, 郭灵安, 李华仙, 雷欣宇, 付成平, 赵珊. 超高效液相色谱-荧光检测法测定水果中3种萘衍生物[J]. 食品工业科技, 2018, 39(14): 200-204. DOI: 10.13386/j.issn1002-0306.2018.14.037
ZHONG Ling-li, LI Xi, GUO Ling-an, LI Hua-xian, LEI Xin-yu, FU Cheng-ping, ZHAO Shan. Determination of 3 Naphthalene-derived Compounds in Fruits by Ultra Performance Liquid Chromatography with Fluorescence Detector[J]. Science and Technology of Food Industry, 2018, 39(14): 200-204. DOI: 10.13386/j.issn1002-0306.2018.14.037
Citation: ZHONG Ling-li, LI Xi, GUO Ling-an, LI Hua-xian, LEI Xin-yu, FU Cheng-ping, ZHAO Shan. Determination of 3 Naphthalene-derived Compounds in Fruits by Ultra Performance Liquid Chromatography with Fluorescence Detector[J]. Science and Technology of Food Industry, 2018, 39(14): 200-204. DOI: 10.13386/j.issn1002-0306.2018.14.037

超高效液相色谱-荧光检测法测定水果中3种萘衍生物

基金项目: 

成都市科技局高校院所应用基础与成果转化项目(12DXYB284NC-005)。

详细信息
    作者简介:

    仲伶俐(1982-),女,硕士,助理研究员,研究方向:食品安全检测,E-mail:17916029@qq.com。

  • 中图分类号: TS255.7

Determination of 3 Naphthalene-derived Compounds in Fruits by Ultra Performance Liquid Chromatography with Fluorescence Detector

  • 摘要: 建立了超高效液相色谱-荧光检测法同时测定水果中1-萘乙酰胺(NAD)、1-萘乙酸(NAA)和2-萘氧乙酸(2-NOA)3种萘衍生物的方法。样品经2%甲酸-乙腈提取,QuEChERS法净化,用ACQUITY UPLC BEH phenyl色谱柱分离,超高效液相色谱荧光检测器测定。3种化合物质量浓度在0.005~1.0 mg/L范围内,与色谱峰面积呈良好的线性关系,相关系数(r)>0.9999。当样品中添加水平为0.01、0.1和1.0 mg/kg时,回收率为78.3%~95.3%,相对标准偏差(RSD)为2.8%~9.5%,方法检出限(LOD)为0.002~0.003 mg/kg,方法定量限(LOD)为0.006~0.01 mg/kg。该方法简便、灵敏、准确,适合用于水果中NAD、NAA、2-NOA的同时测定。
    Abstract: A method for simultaneous determination of 3 naphthalene-derived compounds(1-naphthyl acetamide(NAD),1-naphthalene acetic acid(NAA)and 2-naphthoxy acetic acid(2-NOA))in fruits by ultra performance liquid chromatography with fluorescence detector was developed. The sample was extracted with 2% formic acid-acetonitrile and purified by QuEChERS method. The analytes were separated on ACQUITY UPLC BEH phenyl column and determined by ultra performance liquid chromatography with fluorescence detector. The results showed that there are good linearities between the peak areas and the concentrations in the range of 0.005~1.0 mg/L,with correlation coefficients(r)more than 0.9999. Recoveries of 4 compouds ranged from 78.3% to 95.3% when the spiked levels of 0.01,0.1 and 1.0 mg/kg,with the relative standard deviations(RSDs)were 2.8%~9.5%. The limits of detection(LODs)of the developed method were 0.002~0.003 mg/kg,and the limits of quantitation(LOQs)were 0.006~0.01 mg/kg. The method was simple,sensitive and accurate for simultaneous determination of NAD、NAA and 2-NOA in fruits.
  • 期刊类型引用(2)

    1. 毛琼丽,石豪,严玲,朱艳,陈彦和,李先芝. 超高效液相色谱仪的应用及前景展望. 化学分析计量. 2021(11): 95-102 . 百度学术
    2. 陈妍方,赵月亮,张娜娜,范大明,李利洁,赵建新,张灏. PhIP化学模型体系中前处理及UPLC-MS检测方法的研究. 食品工业科技. 2019(15): 254-258+291 . 本站查看

    其他类型引用(0)

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  • PDF下载量:  4
  • 被引次数: 2
出版历程
  • 收稿日期:  2017-08-24
  • 网络出版日期:  2020-11-26
  • 刊出日期:  2018-07-14

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