燕麦多肽降血糖功能的研究

张慧娟; 黄莲燕; 尹梦; 王静

doi:10.13386/j.issn1002-0306.2017.10.061

燕麦多肽降血糖功能的研究

北京食品营养与人类健康高精尖创新中心,北京市食品添加剂工程技术研究中心

基金项目:

国家自然科学基金项目(31401522)；北京市教育委员会面上项目(KM201510011006)；

详细信息

作者简介:
张慧娟 (1983-) , 女, 博士, 副教授, 研究方向:农副产品深加工, E-mail:zhanghuijuan@th.btbu.eud.cn。;

王静 (1976-) , 女, 博士, 教授, 研究方向:食品功能性配料, E-mail:wangjing@th.btbu.eud.cn。;

中图分类号: R285.5
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出版历程
- 收稿日期: 2016-10-13

Research on hypoglycemic function of oat peptides

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University (BTBU)

摘要

摘要: 目的:探究燕麦多肽对小鼠体内降血糖的功能。方法:实验采用ICR小鼠进行糖尿病造模,对燕麦多肽干预后小鼠的降血糖相关指标进行了研究。将90只雌性ICR小鼠随机分为6组,正常对照组、模型组、阳性组(二甲双胍0.6 g/kg)和燕麦多肽高、中、低剂量组(燕麦多肽1、0.5、0.25 g/kg),连续灌胃28 d。并对小鼠的体重、饮食、饮水、血糖水平、胰岛素含量和肝糖原含量进行测定。结果:燕麦多肽组小鼠体重缓慢增加,与模型组相比,燕麦多肽高剂量组从第2周开始进食量显著降低(p<0.05),第3周开始饮水量显著降低(p<0.05),高剂量组小鼠血糖在给药后第2~4周极显著降低(p<0.01),中剂量组小鼠血糖在给药后第3~4周极显著降低(p<0.01),且4周后中、高剂量组肝糖原含量显著提高(p<0.05),高剂量组胰岛素含量显著提高(p<0.05)。结论:燕麦多肽可增加糖尿病小鼠体重,改善其多饮多食的症状。
- 燕麦 /
- 多肽 /
- 降血糖
Abstract: Objective: To investigate the hypoglycemic effect of oat peptides in rats. Methods: In this research, hypoglycemic functions of oat peptides were studied in vivo. Using the ICR diabetes mice, hypoglycemic related indicators after peptides intervention were tested.90 female ICR rices were divided into six groups randomly: nomal control group, model group ( DMBG0.6 g/kg) , low-dose oat peptides group ( oat peptides 0.25 g/kg) , medium-dose oat peptides group ( oat peptides 0.5 g/kg) , high-dose oat peptides group ( oat peptides 1 g/kg) , continuous filling stomach for 28 days. The level of weight, diet, drinking water, blood glucose, insulin and liver glycogen were measured.Results: The weight of mice in the oat peptides groups were all increased slowly.Compared with the model group, the food-intake in the high-dose oat peptides group were significantly induced from the second week ( p < 0.05) , and the water consumption in the high-dose oat peptides group were significantly induced from the thind week ( p < 0.05) , blood sugar was significantly decreased from the second week to fourth week ( p <0.01) and from the third week to fourth week in the medium-dose group ( p < 0.01) .At the end of the fourth week mice in the high-dose and medium-dose group, its hepatic glycogen content were significantly increased ( p < 0.05) , and insulin level were significantly improved in the high-dose group ( p < 0.05) . Conclusion: Oat peptides could increase mice body weight, and improve its diabetes symptoms of food intake.
- oat /
- peptides /
- hypoglycemic

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参考文献(16)

[1]	苏日娜.中国燕麦产业发展研究[D].内蒙古:内蒙古农业大学, 2013.
[2]	钟婷.燕麦的营养保健功能与综合利用[J].首都医药, 2012 (14) :11-12.
[3]	吴冬梅, 唐良艳, 黄志玲, 等.几种多肽在大鼠立体小肠中的吸收[J].药物生物技术, 2007, 14 (5) :356-360.
[4]	陈飞平.苋籽ACE抑制肽的分离纯化及其性质评价[D].重庆:西南大学, 2013.
[5]	罗美琪.丝素肽的酶解制备及其生物活性的研究[D].广州:华南理工大学, 2012.
[6]	Jin J, Huang W L, Rao G L.Total Synthesis of MC2, a Peptides from Momordica Charantia and Its Hypoglycemic Activity[J].Chinese Journal of Natural Medicines, 2011, 9 (1) :58-60.
[7]	刘雪峰, 李磊, 闫文亮.杏仁多肽的降血糖活性研究[J].内蒙古农业大学学报, 2012, 31 (2) :204-208.
[8]	黄凤杰, 吉静娴, 钱璟.鹿角脱盘多肽的分离纯化及其降糖活性的研究[J].药物生物技术, 2010, 17 (2) :151-156.
[9]	袁晓晴, 顾小红, 汤坚.水溶性癞葡萄降血糖多肽MC2-1-5的分离纯化[J].安徽农业科学, 2007, 35 (31) :9819-9821.
[10]	Mamdouh A, Mohamed E H, Rasha A.Effects of honey, sucrose and glucose on blood glucose and C-peptides in patients with type 1 diabetes mellitus[J].Complementary Therapies in Clinical Practice, 2013, 19 (1) :15-19.
[11]	Chaluntorn V, Kiyotaka N, Phumon S.A novel gelatin crosslinking method retards release of mulberry 1-deoxynojirimycin providing a prolonged hypoglycaemic effect[J].Food Chemistry, 2012, 134 (4) :1823-1830.
[12]	Kwona D Y, Daily J W, Kim H J, et al.Antidiabetic effects of fermented soybean products on type 2 diabetes[J].Nutrition Research, 2010, 30 (1) :1-13.
[13]	Dong J L, Cai F L, Shen R L, et al.Hypoglycaemic effects and inhibitory effect on intestinal disaccharidases of oat betaglucan in streptozotocin-induced diabetic mice[J].Food Chemistry, 2011, 129 (3) :1066-1071.
[14]	Shalaby M A, Latif H A, Sayed M E.Interaction of insulin with prokinetic drugs in STZ-induced diabetic mice[J].World Journal of Gastrointestinal Pharmacology and Therapeutics, 2013, 4 (2) :28-38.
[15]	Trinder P.Determination of blood glucose using an oxidaseperoxidase system with a non-carcinogenic chromogen[J].Journal of Clinical Pathology, 1969, 22 (2) :158-161.
[16]	Qin L, Zhu B W, Zhou D Y, et al.Preparation and antioxidant activity of enzymatic hydrolysates from purple sea urchin (Strongylocentrotus nudus) gonad[J].LWT-Food Science and Technology, 2011, 44 (4) :1113-1118.