A pilot study on sterilization of functional Monascus red rice by steam explosion
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摘要: 目的:初步研究汽爆功能性红曲的灭菌效果以及对其活性物质莫纳可林K(Monacolin K)的影响。方法:汽爆功能性红曲以蒸汽为汽爆介质,研究115、125、135℃温度下分别维压0、1、3、5 min,功能性红曲中Monacolin K含量、水分含量、菌落总数的变化,采用灭菌指示胶带图像处理法评价灭菌效果。结果:汽爆灭菌在达到灭菌要求的情况下(135℃汽爆3 min),相比较传统高压蒸汽灭菌条件121℃、20 min,汽爆后功能性红曲含水量和Monacolin K的损失率分别为13.07%和3.92%,均低于传统高压蒸汽灭菌后的含水量(18.49%)和损失率(17.76%),该汽爆条件下灭菌指示胶带热敏区域灰度平均值为62.77。结论:汽爆灭菌对于功能性红曲是可行且高效的,该汽爆工艺节省红曲产品后续干燥的能量,降低了生产成本,为功能性红曲有效灭菌、保留活性成分以及减少灭菌后成品含水量的汽爆灭菌提供理论依据。Abstract: Objective: To study the effect of steam explosion sterilization on functional Monascus red rice and its main active component Monacolin K.Methods: Functional Monascus red rice was sterilized by steam explosion.The effects of steam explosion residence time 0 min, 1 min, 3 min, 5 min on sterilization efficiency were investigated under 115 ℃, 125 ℃, 135 ℃, respectively.The change of Monatinlin K content, water content and aerobic plate count was investigated, and sterilization indicator tape was used to characterize the sterilization effect.Results: The results showed that the heat ( 3.92%) of Monacolin K sterilized by steam explosion at 135 ℃ for 3 min was lower than the loss ( 17.76%) of traditional sterilization at 121 ℃ for 20 min. And the water content of functional Monascus red rice sterilized by steam explosion was 13.07%, which was lower than the water content ( 18.49%) of traditional sterilization.Under this condition, the average gray value of the sensitive area in sterilization indicator tape was 62.77.Conclusion: Steam explosion was feasible and efficient for the sterilization of functional Monascus red rice, which could greatly reduce the product cost and energy of subsequent dry step. Steam explosion sterilization of functional Monascus red rice could provide the theoretical basis of retention of the active ingredient and reduction of the water content.
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Keywords:
- functional Monascus red rice /
- steam explosion /
- sterilization /
- Monacolin K
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[1] 沈珺珺, 曾柏全, 王卫, 等.红曲米的代谢功能及其应用研究进展[J].食品与机械, 2014 (5) :294-298. [2] 杨宗渠, 朱军, 宋卫东, 等.红曲米粉安全性控制技术[J].食品科学, 2007, 28 (3) :121-124. [3] 陈泉, 吴远征, 赵晓燕, 等.功能性红曲中三类主要聚酮类化合物合成途径相关基因研究进展[J].中国酿造, 2014, 33 (8) :10-14. [4] 孙伟, 刘爱英, 梁宗琦.红曲中莫纳可林K (Monacolin K) 的研究进展[J].西南农业学报, 2003, 16 (3) :112-116. [5] 王文风, 袁兵兵, 徐玲.红曲的研究现状[J].发酵科技通讯, 2014 (1) :39-44, 50. [6] 王锦花, 李易好, 包伯荣, 等.辐照灭菌对红曲粉品质的影响[C]//全国辐照加工技术研讨会论文集.2005. [7] 黄惠兴, 王平原, 邓远玲, 等.微生物实验室培养基高压蒸汽灭菌效果的监测[J].职业与健康, 2012, 28 (10) :1223-1225. [8] 李向科, 潘厚昌, 王绍宇.抗生素发酵液体培养基的灭菌动力学及工艺研究[J].化工与医药工程, 2013, 34 (1) :8-16. [9] 缑三虎, 刘杰, 何苗, 等.红曲中洛伐他汀的分离鉴定和稳定性评价[J].中成药, 2016, 38 (5) :1184-1187. [10] 陈蕴, 陈晔, 许赣荣.烘干和辐照对功能性红曲Monacolin K含量的影响[J].食品与生物技术学报, 2008, 27 (4) :16-19. [11] 李蕙蕙, 易涛, 彭立军, 等.辐照对红曲米中桔霉素及Monacolin K含量的影响[J].食品科技, 2016 (4) :266-270. [12] 陈洪章.现代固态发酵技术:理论与实践[M].化学工业出版社, 2013. [13] 唐超.辐照食品国内外发展现状[J].品牌与标准化, 2011 (2) :19. [14] Liu Z H, Qin L, Pang F, et al.Effects of biomass particle size on steam explosion pretreatment performance for improving the enzyme digestibility of corn stover[J].Industrial Crops&Products, 2013, 44 (2) :176-184.
[15] Zhao Z M, L Wang, H Z Chen.A novel steam explosion sterilization improving solid-state fermentation performance.Bioresource Technology, 2015, 192 (5) :547-555.
[16] Overend R P, Gascoigne J A.Fractionation of Lignocellulosics by Steam-Aqueous Pretreatments[J].Philosophical Transactions of the Royal Society a Mathematical Physical&Engineering Sciences, 1987, 321 (1561) :523-536.
[17] 梅乐和, 姚善泾, 林东强.生化生产工艺学 (第2版) [M].北京:科学出版社, 2007. [18] QB/T 2847-2007, 轻工行业标准功能性红曲米 (粉) [S]. [19] GB 4789.2-2010, 食品安全国家标准食品微生物学检验菌落总数测定[S]. [20] 莫玲.基于RGB彩色空间的图像分割研究[J].计算机科学, 2016, 43 (s1) :168-170. [21] 吴掬鸥, 袁晓桂.基于阈值分割技术的图像分割法研究[J].现代电子技术, 2016, 39 (16) :105-107. [22] Couri S, Mercês E P, Neves B C V, et al.Digital image processing as a tool to monitor biomass growth in Aspergillus niger, 3T5B8 solid-state fermentation:preliminary results[J].Journal of Microscopy, 2006, 224 (3) :290-297.
[23] Shull J J, Cargo G T, Ernst R R.Kinetics of Heat Activation and of Thermal Death of Bacterial Spores[J].Applied Microbiology, 1963, 11 (6) :485-487.
[24] Abraham G, Debray E, Candau Y, et al.Mathematical Model of Thermal Destruction of Bacillus stearothermophilus Spores[J].Applied&Environmental Microbiology, 1990, 56 (10) :3073-3080.
[25] 靳登超, 李阳, 鲍振博, 等.医疗废物处理中生物指示剂湿热灭菌动力学方程[J].环境工程学报, 2015, 9 (9) :4531-4534. [26] Rajan S, Pandrangi S, Balasubramaniam V M, et al.Inactivation of Bacillus stearothermophilus, spores in egg patties by pressure-assisted thermal processing[J].LWT-Food Science and Technology, 2006, 39 (8) :844-851.
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