模拟缫丝废水中丝胶蛋白的树脂吸附分离

焦瑞婷; 江虹锐; 王成华; 赵谋明; 刘小玲

doi:10.13386/j.issn1002-0306.2017.10.036

模拟缫丝废水中丝胶蛋白的树脂吸附分离

广西大学轻工与食品工程学院

基金项目:

广西重大基金项目(2016JJE120003)；

详细信息

作者简介:
焦瑞婷 (1989-) , 女, 硕士研究生, 研究方向:蛋白质资源的开发利用, E-mail:18275796165@163.com。;

刘小玲 (1972-) , 女, 博士, 教授, 研究方向:生物大分子结构与性质, E-mail:13877173857@163.com。;

中图分类号: TQ936;X791
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出版历程
- 收稿日期: 2016-11-06

Adsorption separation of sericin from simulation filature wastewater by resin

College of Light Industry and Food, Guangxi University

摘要

摘要: 本文比较AB-8、LX-1180、D101、DA201-C、DEAE-52、201×7六种树脂对模拟缫丝废水中丝胶蛋白的静态吸附和解析性能,筛选出适宜分离纯化丝胶蛋白的树脂,并通过静态和动态洗脱实验确定最佳工艺参数。结果表明:DEAE-52型离子交换树脂吸附解析效果最好,吸附量为1.573 mg/g,吸附率可达到95.07%。柱分离时,丝胶废液以5 BV/h流入分离柱;采用p H2的0.05 mol/L的氯化钠溶液以2 BV/h洗脱4 BV,收集1.7³.0 BV洗脱流分,洗脱率98.76%,经吸附分离,丝胶蛋白浓缩倍数达到16.33,收集所得丝胶蛋白的回收率和纯度分别可达95.26%和96.71%。
- 缫丝废水 /
- 丝胶蛋白 /
- 树脂 /
- 吸附
Abstract: The sericin adsorption and desorption ability from simulation filature of six types of resins, AB-8, LX-1180, D101, DA201-C, DEAE-52 and 201 × 7 were compared and the best resin was selected. The optimal sericin separation parameters were determined by static and dynamic absorption experiment.The results showed that sericin adsorption capacity and recoverity of DEAE-52 ion exchange resin were much higher than other resins reached 1.573 mg/g and 95.07%, respectively.During the column separation, filature wastewater flowed with 5 BV/h into column, and then was eluted with 4 BV of p H2, 0.05 mol/L sodium chloride solution by 2 BV/h, then 1.7 ~ 3.0 BV elution fractions was collected, the desorption ratio was 98.76%. After column separation, the recoverd sericin concentrated 16.33 times of wastewater, and the total recovery rate and purity of collected sericin were 95.26% and 96.71%, respectively.
- filature wastewater /
- sericin /
- resin /
- adsorption

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