人母乳源乳酸菌的筛选、鉴定及益生活性的初步研究

高盛; 乔宇; 张宇微; 彭晴; 张志胜; 石波; 李静梅; 吴颖

doi:10.13386/j.issn1002-0306.2017.10.031

人母乳源乳酸菌的筛选、鉴定及益生活性的初步研究

高盛¹,
乔宇²,
张宇微²,
彭晴²,
张志胜¹,
石波²,
李静梅³,
吴颖⁴

1. 河北农业大学食品科技学院
2. 中国农业科学院饲料研究所
3. 中国农业科学院作物科学研究所
4. 北京市产品质量监督检验院

基金项目:

中国农业科学院科技创新工程(FRI-06)；

详细信息

作者简介:
高盛 (1990-) , 女, 硕士研究生, 研究方向:母乳微生物的资源的发掘与利用, E-mail:794451925@qq.com。;

乔宇 (1979-) , 女, 博士, 副研究员, 研究方向:功能性寡糖与微生物互作的机理研究, E-mail:qiaoyu@caas.cn。;

张志胜 (1970-) , 男, 博士, 教授, 主要从事畜产品加工原理及技术方面的研究, E-mail:13833035679@139.com。;

中图分类号: TS201.3
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- 收稿日期: 2016-10-17

Isolation, identification and probiotic characterization of lactic acid bacteria in human breast milk

College of Food Science and Technology, Agricultural University of Hebei
Feed Research Institute Chinese Academy of Agricultural Sciences
Institute of Crop Science Chinese Academy of Agricultural Sciences
Beijing Products Quality Supervision and Inspection Institute

摘要

摘要: 本研究从人母乳中分离乳酸菌。采用透明圈法从产后10³0 d的人母乳样品中筛选到一株产酸能力较强的乳酸菌。对筛选菌株进行形态学观察、生理生化实验及16S r DNA序列鉴定。结果发现,该菌株为革兰氏阳性,短杆状,无芽孢;生理生化特性符合鼠李糖乳杆菌的基本特征;16S r DNA序列及进化树分析进一步确认该菌株为鼠李糖乳杆菌,命名为鼠李糖乳杆菌Z5。在p H3.0的条件下模拟人工胃肠液共消化6 h后该菌存活率仍达90%以上,在0.15 g/L胆盐质量浓度培养6 h,菌株存活率为76.14%;抑菌实验发现,菌株培养液对沙门氏菌及大肠杆菌的生长有明显的抑制作用,抑菌圈直径达20 mm以上。细胞黏附实验发现,该菌株可有效黏附到Caco-2细胞表面,对Caco-2细胞的平均黏附数为7.54×105cfu/m L。从人母乳中获得了这株具有潜在益生性质的鼠李糖乳杆菌,为明确母乳中益生菌的功效及开发新型益生菌产品研究提供了良好的实验材料。
- 人母乳 /
- 鼠李糖乳杆菌 /
- 分离鉴定 /
- 益生性质
Abstract: The strains producing acid were isolated in this study. The transparent ring method on MRS-Ca CO₃ medium plates were used to isolate a bacterial strain producing more acid from the 10 ~30 d breast milk after giving birth. The optimal strain was identified by morphological observation, physiological and biochemical tests and 16 S r DNA sequences, and it presented Gram positive, producing more lactic acid, rod-shaped and non-spore forming. The results of physiological and biochemical tests were consistent with Lactobacillus rhamnosus, named Z5. When the strain was incubated in artificial gastric juice and intestinal juice with p H3.0 for 6 h, the survival rates were more than 90%. After 6 h incubation in 0.15 g/L bile salt, the survival rate for the strain was 76.14%.The growth of Salmonella pullorum and Escherichia coli were inhibited by the cultures of the strain Z5 and the antibacterial circle diameter was greater than 20 mm.The strain Z5 could effectively adhered to the Caco-2cells and the average number of adhesion was 7.54 × 105cfu/m L. These results suggested that Lactobacillus rhamnosus Z5 isolating from human breast milk showed the high survival rate in gastrointestinal environments and could be a good candidate for probiotic application.
- breast milk /
- Lactobacillus rhamnosus /
- isolation and identification /
- probiotic

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人母乳源乳酸菌的筛选、鉴定及益生活性的初步研究

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Isolation, identification and probiotic characterization of lactic acid bacteria in human breast milk

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