Effect of different vegetable oils on the qualities, flavors and fermentation characteristics of probiotic yoghurt
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摘要: 本研究的目的在于探讨添加四种植物油对脱脂酸奶发酵过程中理化性质、质构、挥发性风味成分及感官品质的影响;实验以双歧杆菌、保加利亚乳杆菌、嗜酸乳杆菌和嗜热链球菌为发酵剂制备凝固型益生菌酸奶,测定了p H、滴定酸度及持水性,并进行了质构分析和感官评价,采用固相微萃取法(HS-SPME)结合气相色谱质谱联用技术(GCMS)对后熟24、48及96 h的酸奶中挥发性风味物质进行鉴定。结果表明:添加大豆油、花生油与核桃油显著降低酸奶p H(p<0.01),添加大豆油与花生油显著提高酸奶滴定酸度值(p<0.05),添加核桃油和葡萄籽油显著提高了酸奶持水性(p<0.05),添加四种植物油均显著降低了酸奶的硬度与稠度,提高了粘聚性和粘性指数(p<0.05);同时,随着酸奶后熟时间的延长,酸奶中挥发性风味物质相对含量发生变化,鉴定出来自植物油的挥发性风味物质;感官评价结果表明植物油的添加对酸奶色泽影响不显著(p>0.05),但对气味、滋味、组织状态影响显著(p<0.05)。本研究为生产富含不饱和脂肪酸的益生菌酸奶提供理论依据。Abstract: The objectives of this study were to evaluate the texture characteristics, volatile flavor compounds and sensory quality of yoghurt after adding four kinds of vegetable oil to fermentation process of skim yogurt. Bifidobacterium, Lactobacillus bulgaricus and Streptococcus thermophilus were used as starter and the p H values, titratable acidity, water holding capacity, texture analysis and sensory evaluation were determined. Volatile flavor compounds in yogurt were detected using solid phase micro-extraction ( HS-SPME) combined with gas chromatography mass spectrometry hyphenated technique ( GC-MS) after ripening and at 24, 48 or 96 h.The results showed that the addition of soybean oil, peanut oil and walnut oil could significantly reduce the yoghurt p H values ( p < 0.01) . In addition, the addition of soybean oil and peanut oil could significantly increase titratable acidity values ( p < 0.05) and the addition of walnut oil and grape seed oil could significantly improve the water holding capacity ( p < 0.05) , but the addition of four kinds of vegetable oils could significantly reduce the firmness and consistency ( p <0.05) , while the cohesiveness and index of viscosity were improved. Meanwhile, the relative contents of volatile flavor compounds in yogurt changed and some vegetable oil components were identified with the extension of the ripening time of yoghurt.Sensory evaluation results showed that added vegetable oil did not significant influence the color of the yogurt ( p >0.05) , but affected smell, taste and texture significantly ( p < 0.05) .This study provides useful information for the development of new products which rich in unsaturated fatty acids.
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Keywords:
- vegetable oil /
- skim milk /
- physicochemical property /
- texture /
- volatile flavor compounds /
- sensory quality
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[1] 王丽凤, 张和平.益生菌、胃肠道微生物和宿主之间相互作用的研究进展[J].中国食品学报, 2011 (4) :147-153. [2] Maslova E, Halldorsson T I, Strm M, et al.Low-fat yoghurt intake in pregnancy associated with increased child asthma and allergic rhinitis risk:a prospective cohort study.[J].Journal of Nutritional Science, 2012, 1 (1) :315.
[3] 魏月媛.火麻仁油的安全性及在酸奶中的应用研究[D].广州:华南理工大学, 2015. [4] 杨春英, 刘学铭, 陈智毅.15种食用植物油脂肪酸的气相色谱-质谱分析[J].食品科学, 2013 (6) :211-214. [5] 熊秋芳, 张效明, 文静, 等.菜籽油与不同食用植物油营养品质的比较——兼论油菜品质的遗传改良[J].中国粮油学报, 2014 (6) :122-128. [6] 姜梅, 董明盛, 芮昕, 等.高压均质和热处理对豆乳蛋白质溶解性的影响[J].食品科学, 2013 (21) :125-130. [7] 王攀.酪蛋白对凝固型羊奶酸奶发酵特性的影响[J].中国乳业, 2012 (6) :66-68. [8] 祝静.羊酸奶发酵工艺及其质构特性和风味成分研究[D].杨凌:西北农林科技大学, 2011. [9] 郭文奎.优质酸奶风味图谱的建立[D].哈尔滨:东北农业大学, 2012. [10] 王克建, 郝艳宾, 齐建勋.核桃油研究进展[J].食品科学, 2004 (11) :364-367. [11] Munzur M M, Islam M N, Akhter S, et al.Effect of different levels of vegetable oil for the manufacture of Dahi from skim milk[J].Asian Australasian Journal of Animal Sciences, 2004, 17 (7) :1019-1025.
[12] Barrantes E, Tamime A Y, Sword A M, et al.The manufacture of set-type natural yoghurt containing different oils-2:Rheological properties and microstructure[J].International Dairy Journal, 1996, 6 (8) :827-837.
[13] 孔保华.乳品科学与技术[M].北京:科学出版社, 2006. [14] 阳丽芝, 陈志伟.牛奶中脂肪检测技术研究进展[J].食品科学, 2011 (1) :270-273. [15] 陈洁, 赵建新, 陈卫, 等.中温发酵酸乳的游离氨基酸、脂肪酸和感官特性的研究[J].食品与发酵工业, 2008 (7) :28-33. [16] Kalua C M, Allen M S, Jr D R B, et al.Olive oil volatile compounds, flavour development and quality:A critical review[J].Food Chemistry, 2007, 100 (1) :273-286.
[17] Beshkova D, Simova E, Frengova G, et al.Production of flavour compounds by yogurt starter cultures[J].Journal of Industrial Microbiology&Biotechnology, 1998, 20 (3) :180-186.
[18] 王伟君, 李延华, 张兰威, 等.SDE-GC-MS法测定发酵乳中风味物质[J].食品科学, 2008 (6) :332-334. [19] 孙静, 黄沁怡, 李芳, 等.应用化学传感器和GC-MS研究加热温度与大豆油挥发物质的关系[J].中国粮油学报, 2013 (1) :122-128. [20] Steenson D F, Lee J H, Min D B.Solid Phase Microextraction of Volatile Soybean Oil and Corn Oil Compounds[J].Journal of Food Science, 2002, 67 (1) :71-76.
[21] 李苑雯, 陈素娟, 柏文良, 等.花生油香气特征在其属性识别中的初步应用[J].食品与发酵工业, 2013 (7) :193-199. [22] 刘晓君, 金青哲, 刘元法, 等.花生油挥发性风味成分的鉴定[J].中国油脂, 2008 (8) :40-42. [23] Shrestha K, Meulenaer B D, Gemechu F G.A novel insight on the high oxidative stability of roasted mustard seed oil in relation to phospholipid, Maillard type reaction products, tocopherol and canolol contents[J].Food Research International, 2013, 54 (1) :587-594.
[24] 徐月华.冷榨核桃油的香味强化及核桃乳稳定性的研究[D].无锡:江南大学, 2014. [25] 杨朝晖, 马磊, 孟阿会, 等.固相微萃取GC/MS法测定氧化核桃油中挥发性成分[J].农业机械, 2011 (26) :59-60. [26] Tavaria F K, Dahl S, Carballo F J, et al.Amino Acid Catabolism and Generation of Volatiles by Lactic Acid Bacteria[J].Journal of Dairy Science, 2002, 85 (10) :2462-2470.
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