Effects of drying methods on the juglone content and antibacterial activity of green walnut husk
-
摘要: 为考察核桃青皮抑菌活性与干燥方式的关系,以大肠杆菌、金黄色葡萄球菌和石榴干腐病菌为供试菌,评价了自然干燥（P1）、烘干（P2）及真空冷冻干燥（P3）核桃青皮的抑菌活性。结果显示,3种样品对3种供试菌均有较强的抑制作用,并呈现剂量依赖效应,即M1>M2>M3（M1,M2,M3浓度分别为100、80和60 mg/m L的核桃青皮萃取液）,3种干燥方式间比较均表现为P3>P2>P1;P1、P2和P3对大肠杆菌和石榴干腐病菌的最小抑菌浓度（MIC）结果一致,分别为20、15和10 mg/m L,对金黄色葡萄球菌的MIC分别是15、10和5 mg/m L;P1、P2和P3样品中胡桃醌的含量分别是8.04、9.46和11.84 mg/g,与其抑菌活性呈极显著正相关（p<0.01）。表明干燥方式会影响核桃青皮中胡桃醌含量及抑菌活性,其中真空冷冻干燥是一种理想的干燥方式。Abstract: To explore the relationship between dry method and antibacterial activity of green walnut husk, the antibacterial activities of natural drying ( P1) , hot-air drying ( P2) and vacuum freeze drying ( P3) of green walnut husk were evaluated, using Escherichia coli, Staphylococcus aureus and Zythia versoniana Sacc.as test microorganisms in this paper.Results showed that the three samples all had strong inhibition, and presented a dose-response relationship, namely M1> M2> M3 ( dose of walnut green husk extract were respectively 100 ( M1) , 80 ( M2) , and 60 ( M3) mg/m L) .The drying methods were characterized by P3> P2>P1.For P1, P2 and P3, the minimum inhibition concentration ( MIC) of E.coli and Zythia versoniana Sacc.were consistent.The doses were 20, 15 and 10 mg/m L respectively.The MIC of Staphylococcus aureus were 15, 10, and 5 mg/m L.The juglone content of samples were respectively 8.04 ( P1) , 9.46 ( P2) , and 11.84 ( P3) mg/g, significant positive correlation with antibacterial activity ( p < 0.01) .In conclusion, the drying methods could affect the juglone content and antibacterial activity of green walnut husk, vacuum freeze drying was an ideal way.
-
Keywords:
- green walnut husk /
- drying method /
- juglone /
- antibacterial activity
-
[1] 高海生, 朱凤妹, 李润丰.我国核桃加工产业的生产现状与发展趋势[J].经济林研究, 2008, 26 (3) :119-126. [2] 孙桂君, 苑淑莉.青龙衣糖膏治疗浅表性胃炎疗效观察[J].中医药学报, 2002, 30 (3) :16. [3] 赵岩, 刘淑萍, 吕朝霞.核桃青皮的化学成分与综合利用[J].农产品加工, 2008, 7 (11) :66-68. [4] 潘富赟, 张培正.核桃青皮的综合应用及开发前景[J].中国食物与营养, 2010, 135 (12) :21-24. [5] 景炳年.核桃次生物质抗烟草花叶病毒活性的研究[D].杨陵:西北农林科技大学, 2007. [6] 黄支权, 任建军, 成军, 等.核桃青皮抑真菌物质的分离鉴定与抑菌活性[J].北京农学院学报, 2016, 31 (1) :23-27. [7] Li C, Liu J X, Zhao L, et al.Capillary zone electrophoresis for separation and analysis of four diarylheptanoids and an alphatetralone derivation in the green walnut husks (Juglans regia L.) [J].Journal of Pharmaceutical and Biomedical Analysis, 2008, 48 (3) :749-753.
[8] Nour V, Trandafir I, Cosmulescu S.HPLC determination of phenolic acids, flavonoids ang juglone in walnut leaves[J].Journal of Chromatographic Science, 2013, 51:883-890.
[9] 田平平, 李仁宙, 简永健, 等.核桃青皮的强抗氧化活性成分及其抗氧化稳定性[J].中国农业科学, 2016, 49 (3) :543-553. [10] 林君阳, 马良进, 陈安良, 等.山核桃外果皮化学成分及抑菌活性初步研究[J].浙江林学院学报, 2009, 26 (1) :100-104. [11] 许绍惠, 唐婉屏, 韩忠环.核桃楸毒性成分研究[J].沈阳农业大学学报, 1986, 17 (2) :34-39. [12] Yasmin A, Murray B I, Lee C H, et al.Toxicity of quinones against two-spotted spider mite and three species of aphids in laboratory and greenhouse conditions[J].Industrial Crops and Products, 2012, 37:536-541.
[13] 欧阳瑾, 曹志友, 王爽, 等.核桃青皮提取物抑小鼠Lewis肺癌生长的实验研究[J].时珍国医药, 2009, 20 (11) :2749-2750. [14] 张巍, 吕士杰.胡桃醌诱导肿瘤细胞凋亡机制[J].解剖学报, 2015, 46 (5) :641-645. [15] 孙墨珑, 王艳梅, 宋湛谦, 等.核桃楸乙醇提取物的稳定性[J].东北林业大学学报, 2008, 36 (5) :53-54. [16] 季宇彬, 陆婉, 曲中原, 等.不同干燥方法、贮藏年限及采收时间对青龙衣中胡桃醌的影响[J].现代药物与临床, 2009, 24 (2) :110-112. [17] 张卫星, 何开泽, 蒲蔷.核桃青皮提取物的抗菌和抗氧化活性[J].应用与环境生物学报, 2014, 20 (1) :87-92. [18] 孙墨珑, 袁海舰, 宋湛谦.分光光度法测定核桃楸树皮中胡桃醌的含量[J].东北林业大学学报, 2007, 6 (6) :37-38. [19] 程丽娟, 薛泉宏.微生物学实验技术[M].西安:世界图书出版公司, 2000. [20] 丁存宝, 吴尚卓, 李桂秋.核桃青皮提取物的抑真菌活性研究[J].现代食品科技, 2013, 29 (4) :722-724. [21] 党珍, 周涛.几种植物提取液的抑真菌特性及复配研究[J].食品科学, 2009, 30 (23) :88-91. [22] 乔永刚, 牛颜冰, 乔木, 等.核桃青皮提取物对4种植物枯萎病菌的抑菌作用研究[J].农学学报, 2011, 1 (7) :9-12. [23] 吴朝霞, 张敏, 金楠.胡桃醌对大肠杆菌呼吸及能量代谢的影响[J].食品与机械, 2011, 7 (6) :131-134. [24] 李钧, 曲中原, 邹翔, 等.青龙衣中胡桃醌的分离与鉴定[J].哈尔滨商业大学学报:自然科学版, 2011, 27 (5) :641-644. [25] 王红萍, 尹江艳.核桃青皮的有效成分及综合利用[J].安徽农业科学2013, 41 (24) :10129-10131.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
