Abstract: To analyze the nutritive composition in the muscle of three populations of Monopterus albus( population A means deep yellow and big spots,population B means pale yellow and thin spots and C means steel grey).Moisture,crude protein,fat,ash,amino acids,fatty acids and mineral elements were investigated by national standard method.The moisture,crude protein,fat and ash consisted of 74.77% ~77.20%,17.84% ~18.52%,2.51% ~4.32%,0.96% ~1.01%,respectively.There was significant difference of moisture and crude protein and crude fat in C compared with those in other two populations( p < 0.05).Ash in muscle of C was significantly higher than A( p < 0.05),but for C and B,it had no significantly difference( p > 0.05).There was significant difference of crude protein in A and B( p < 0.05).The total content of amino acids was 16.68~17.99 g /100 g,it had significant difference in C compared with that in other two populations( p < 0.05),but it had no significant difference in A and B( p >0.05). The content of essential amino which was 7.16 ~ 7.25 g /100 g from three populations had no significant difference( p > 0.05),the content of essential amino acids in A and B were higher than that in C.21 kinds of fatty acids were determinated,the total content of eicosapntemacnioc acid and docosahexaenoic acid which in A and B were higher than those in C were high,about 9.47% ~ 9.99%. The content of mineral elements in muscle was abundant,there were significant difference of 10 kinds of elements in C compared with those in other two populations( p < 0.05),only 2 kinds of elements had significant difference in A compared with those in B( p < 0.05). It can be concluded that the nutritive composition in muscle of the two populations with yellow skin had no obviously difference,however,they had significantly difference with C population.