25个南瓜品种不同溶剂提取物抗氧化活性研究

王士苗; 康文艺; 孙丽; 周俊国; 李新峥

doi:10.13386/j.issn1002-0306.2015.07.016

25个南瓜品种不同溶剂提取物抗氧化活性研究

1. 河南科技学院园艺园林学院
2. 河南大学药学院

基金项目:

河南省高校科技创新团队支持计划(2012IRTSTHN016)；

详细信息

作者简介:
王士苗(1989-),女,在读硕士生,研究方向:蔬菜种质资源研究与利用。;

李新峥(1965-),男,学士,教授,主要从事南瓜资源特性与开发利用研究。;

中图分类号: TS255.1
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出版历程
- 收稿日期: 2014-05-18

Antioxidant activity of different solvent extracts from 25 kinds of cultivated Cucurbita Linn.fruits in vitro

School of Horticulture and Landscape Architecture,Henan Institute of Science and Technology
School of Pharmacy,Henan University College

摘要

摘要: 采用清除1,1-二苯基-2-三硝基苯肼（DPPH）自由基、清除[2,2’-连氨-（3-乙基苯并噻唑啉-6-磺酸）二氨盐]（ABTS）自由基以及铁离子还原能力（FRAP）的方法,以BHT为阳性对照药物,研究25个南瓜品种的体外抗氧化活性。结果表明:南瓜提取物清除DPPH自由基能力均较弱;"汕美2号"乙酸乙酯清除ABTS自由基（IC50=（4.41±0.23）μg/m L）能力最强,强于阳性对照BHT（IC50=（8.59±0.56）μg/m L）;对于还原Fe3+能力,乙酸乙酯提取物高于其他部位提取物,其中"改良蜜本"乙酸乙酯部位（TEAC=（529.65±69.52）μmol/g）的还原能力弱于阳性对照Trolox（TEAC=（1387.51±37.93）μmol/g）。
- 南瓜果实 /
- 体外抗氧化 /
- DPPH /
- ABTS /
- FRAP
Abstract: This study was aimed to evaluate the antioxidant activity of 25 kinds of pumpkin extraction in vitro. The scavenging of 1,1-diphenyl-2-picrylhydrazyl( DPPH) radicals and( 2,2'-even the ammonia( 3-ethyl benzene and thiazole-6-sulfonic acid) diamonium salt)( ABTS) radicals assay and ferric reducing capacity( FRAP) assay were used to measure the antioxidant activity of pumpkins. And BHT was used as the positive control drug. Results showed that the scavenging capability of DPPH free radical ability was weak.The ethyl acetate extract of Shanmei II showed the strongest power to clear ABTS radical( IC50=( 4.41 ± 0.23) μg/m L),better than that of BHT( IC50=( 8.59± 0.56) μg/m L).The power of ethyl acetate for reduction of Fe3 +ability was higher than that of other extracts,and Gailiangmiben ethyl acetate extract( TEAC =( 529.65 ± 69.52) μmol/g) was weaker than that of Trolox( TEAC =( 1387.51 ± 37.93) μmol/g).
- pumpkin fruit /
- antioxidant in vitro /
- DPPH /
- ABTS /
- FRAP