Abstract: Random- Centroid Optimization ( RCO) methodology was used to optimize the extraction technology of flavonoids and polyphenols from the Coreopsis tinctoria Nutt.grow in xinjiang.The results showed that Subcritical Water Extraction was a effective methods for flavonoids and polyphenols.By comparing the extraction temperature, solid- liquid ratio and extraction time, the optimum extraction process of flavonoids and polyphenols from the Coreopsis tinctoria Nutt.has been established as follows: extraction temperature 160~170℃, solid- liquid ratioratio0.04, extraction time 47 min.In this condition, the total yields of flavonoids and polyphenols was more than 25.85%, and among them the flavonoids was more than 15.81%, the polyphenols was more than 10.04%. Parameter optimization could be carried out directly without single test by using Random- Centroid Optimization methodology, which had the advantages of simplicity, fewer experiments and flexibility.Meanwhile flavonoids and polyphenols could be extracted simultaneously with subcritical water, which was organic solvent- free and acid-free.That is a green extract technology with high-efficiency, non toxic and environmental-friendly.