Study on preparation of soluble dietary fiber by enzymatic method from kiwifruit and its antioxidant activity
-
摘要: 以猕猴桃皮渣为原料,采用酶法制备猕猴桃可溶性膳食纤维。在单因素实验的基础上,以纤维素酶添加量、酶解时间、酶解温度和液料比为实验因素,以可溶性膳食纤维提取率为响应值,采用四因素五水平的响应面分析法进行实验,优化提取工艺参数。同时,考察了猕猴桃可溶性膳食纤维对DPPH和ABTS+自由基的清除效果及其还原能力。结果表明,酶法制备猕猴桃可溶性膳食纤维的最佳工艺条件为:酶添加量0.86%、酶解时间2.5h、酶解温度62℃和液料比27∶1(mL/g),在该条件下猕猴桃可溶性膳食纤维提取率预测值为13.379%,验证值为12.983%,响应面法对猕猴桃可溶性膳食纤维提取条件的优化是可行的,可用于实际预测。抗氧化活性实验表明猕猴桃可溶性膳食纤维具有较强的自由基清除效果和还原能力,对DPPH和ABTS+自由基的EC50分别为4.68mg/mL和1.28mg/mL。Abstract: The enzymatic method was adopted to extract soluble dietary fiber from the skin residue of kiwifruit.On the basis of one factor test, the method of response surface analysis with 4 factors including the amount of cellulose, extract time, extract temperature and liquid-to-solid ratio on the extraction ratio of soluble dietary fiber was adopted. Meanwhile, the antioxidant activity of the soluble dietary fiber was tested. The result showed that, these optimal extraction conditions were as follows :the amount of cellulose 0.86%, extract time 2.5h, extract temperature 62℃ and the liquid-to-solid ratio 27∶1mL/g, the predicted value and measured value of the soluble dietary fiber were 13.379% and 12.983%, respectively. Results indicated that the obtained mode developed by response surface methodology was feasible for practical prediction. The antioxidant activity test showed that, the soluble dietary fiber from kiwifruit had strong radical scavenging activity and reducing capacity, the EC50 of DPPH and ABTS were 4.68mg/mL and 1.28mg/mL, respectively.
-
[1] 杨艳杰, 白新鹏, 裘爱泳.猕猴桃属植物的研究进展[J].安徽农业科学, 2007, 35 (35) :11454-11457. [2] 李军德.红阳猕猴桃[J].湖北林业科技, 2003 (4) :29. [3] Tavarini S, Degl’lnnocenti D, Remorini D, et al.Antioxidant capacity, ascorbic acid, total phenols and carotenoids changes during harvest ad after storage of Hayward kiwifruit[J].Food Chemistry, 2008, 107:282-288.
[4] 崔莹.猕猴桃属植物化学成分及药理活性研究进展[J].西安文理学院学报:自然科学版, 2011, 14 (4) :21-25. [5] Motohashi N, Shirataki Y, Kawase M, et al.Biological activity of kiwifruit peel extracts[J].Phytotheraphy Research, 2001, 15 (4) :337-343.
[6] 韩俊娟, 木泰华, 张柏林.膳食纤维生理功能的研究现状[J].食品科技, 2008 (6) :243-245. [7] 郑建仙.功能性食品:第1卷[M].北京:中国轻工业出版社, 1995:13-16. [8] 李加兴, 刘飞, 范芳利, 等.响应面法优化猕猴桃皮渣可溶性膳食纤维提取工艺[J].食品科学, 2009, 30 (14) :143-148. [9] 潘曼, 钟海雁, 李忠海, 等.酶法制备猕猴桃渣膳食纤维工艺研究[J].经济林研究, 2009, 27 (1) :29-33. [10] 刘林林, 吴茂玉, 葛帮国, 等.采用响应面法对酶法提取香蕉皮可溶性膳食纤维工艺的优化[J].农产品加工·学刊, 2013 (3) :5-9. [11] Sun Ting, Ho CT.Antioxidant activities of buckwheat extracts[J].Food Chemistry, 2005, 90:743-749.
[12] ST魪PHANIE DUDONN魪, XAVIER VITRAC, PHILIPPE COUTI魬RE, et al.Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC sssays[J].J Agric Food Chem, 2009, 57:1768-1774. [13] Fang ZX, Zhang YH, Yuan L譈, et al.Phenolic compounds and antioxidant capacities of bayberry juices[J].Food Chemistry, 2009, 113:884-888. [14] 杨念, 芮汉明.金针菇提取物的提取工艺优化及其抗氧化性能研究[J].食品与发酵工业, 2011, 37 (1) :194-198. [15] 何余堂, 高虹妮, 解玉梅, 等.超声波协同酶法制备杏仁皮中水溶性膳食纤维及理化研究[J].食品工业科技, 2013, 34 (1) :229-232. [16] 洪华荣.胡萝卜渣膳食纤维提取工艺及其功能性研究[D].福州:福建医科大学, 2007. [17] Wang Jing, Sun B G, Gao Y P, et al.Optimisation of ultrasound-assisted extraction of phenolic compounds from wheat bran[J].Food Chemistry, 2008, 106 (2) :804-810.
[18] Shon M Y, Kim T H, Sung N J.Antioxidants and free radical scavenging activity of Phellinus baumii (Phellinus of Hymenochactaceae) extracts[J].Food Chemistry, 2003, 82:593-597.
-
期刊类型引用(17)
1. 陈昌威,付靖雯,沈祥皓,李湖平,叶静静,谢箭,王灵昭,盘赛昆. 海藻酸钠的流变学特性及影响黏度因素研究. 中国食品添加剂. 2024(01): 99-108 . 百度学术
2. 李明娟,王颖,张雅媛,游向荣,周葵,卫萍,韦林艳. 5种干燥方式对木薯酸淀粉性能的影响. 食品工业科技. 2024(08): 134-142 . 本站查看
3. 刘会佳,刘伟聪,那治国. 絮凝法回收马铃薯淀粉废水中蛋白质工艺优化. 农产品加工. 2024(21): 68-72+79 . 百度学术
4. 惠宇晴,沈卉芳,姚鑫淼,周野,李庆全,孙庆申. 不同品种马铃薯雪花全粉品质分析. 食品工业科技. 2023(19): 356-365 . 本站查看
5. 张婧娟,吴子煜,丁媛,雍雅萍. 马铃薯全粉面条配方的优化. 现代食品. 2023(17): 106-109 . 百度学术
6. 祁芳,刘星,钱群丽,周佳欣,姚春霞,宋卫国. 基于稳定同位素的5种蔬菜产地真实性判别. 中国无机分析化学. 2022(01): 171-177 . 百度学术
7. 徐忠,陈晓明. 植物乳杆菌发酵对马铃薯全粉结构与性能的影响. 食品与发酵工业. 2022(05): 111-115 . 百度学术
8. 刘爽,王滢颖,郭爱良,周晨霞,李慧静. 不同品种马铃薯全粉品质特性分析. 食品工业科技. 2022(07): 59-66 . 本站查看
9. 辛晶,黄一承,季香青,杨定宽,王玉超,张健,李晓磊,李丹. 黍子粉对小麦面团流变特性和面条品质的影响. 食品工业科技. 2022(19): 69-75 . 本站查看
10. 徐茹,杨晓清,刘晓波,米雪. 超声波处理对马铃薯全粉面团网状结构的影响. 中国食品学报. 2022(10): 242-251 . 百度学术
11. 胡安阳,吕建秋,杜冰. 不同干燥方式对柚子皮粉加工特性及功能成分含量的影响. 食品工业科技. 2021(05): 170-176 . 本站查看
12. 施杨琪,黄茜蕊,茹炜岽,张瑜,柴立红,钱琼秋,包劲松. 14种不同马铃薯全粉的理化特性差异分析. 核农学报. 2021(07): 1593-1600 . 百度学术
13. 刘媛,王健,任丛涛,高清海,刘佳,苗雨田. 新型马铃薯雪花粉饼配方优化. 食品工业. 2021(07): 61-64 . 百度学术
14. 李亚,黄敏,吴笛,褚哓锐. 凉山州马铃薯加工发展探究. 南方农业. 2021(21): 144-146 . 百度学术
15. 杨晓清,徐茹. 调质马铃薯全粉的稳态流变特性及面条品质评价. 粮油食品科技. 2021(06): 131-138 . 百度学术
16. 丁香丽,李婷婷,还璐,钱建亚,周晓燕. 响应面法优化无麸质马铃薯曲奇制作工艺. 美食研究. 2020(01): 34-39 . 百度学术
17. 王腾飞,胡志敏,霍梅俊,王志华. 南瓜马铃薯糕加工工艺. 食品工业. 2020(10): 9-13 . 百度学术
其他类型引用(8)
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
- 被引次数: 25