Study on extraction, analysis and antioxidant activity of lipid from the viscera of starfish (Asterina pectinifera)
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摘要: 以黄海海燕内脏为原料,应用四种蛋白酶辅助有机溶剂法提取黄海海燕内脏脂质,采用气相色谱-质谱法分析了脂质的脂肪酸组成,同时评价了脂质对细胞氧化损伤的保护作用。结果表明,碱性蛋白酶组（Alc）、中性蛋白酶组（Nue）、木瓜蛋白酶组（Pap）和胰蛋白酶组（Try）辅助有机溶剂法提取的脂质得率分别为6.1%±0.02%,6.77%±0.06%,7.96%±0.04%和5.48%±0.02%;多不饱和脂肪酸分别占总脂肪酸的50.11%±0.21%,47.67%±3.78%,50.45%±0.76%和40.84%±0.14%,其中二十碳五烯酸和二十二碳六烯酸为主要成分;脂质可以显著抑制过氧化氢引起的细胞氧化损伤,具有一定的体外抗氧化活性。Abstract: The objective of this study was to extract lipid from starfish (Asterina pectinifera) viscera by enzyme assisted solvent method, as well as evaluate its protective effect on cellular oxidative damage. Results indicated that the lipid yields were 6.1%±0.02%, 6.77%±0.06%, 7.96%±0.04% and 5.48%±0.02% by using alkali protease (Alc) , neutral protease (Neu) , papain (Pap) and trypsin (Trp) assisted solvent method, respectively. The fatty acids of the extracted lipid were analyzed by GC-MS. Results showed that the total polyunsaturated fatty acids (PUFAs) accounted for 50.11% ±0.21% (Alc) , 47.67%±3.78% (Neu) , 50.45%±0.76% (Pap) , and 40.84% ±0.14% (Try) of the total fatty acids, respectively. Among them, eicosapentaenoic acid (EPA) and docosahexaenic acid (DHA) were dominant. Moreover, the lipid from starfish viscera showed a certain cellular antioxidant activity and protective effect on oxidative damage of the liver cells.
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