酸水解处理对荷叶抗氧化活性的影响

doi:10.13386/j.issn1002-0306.2012.18.061

酸水解处理对荷叶抗氧化活性的影响

基金项目:

中国博士后科学基金资助项目(20100481099)；江西省教育厅科技项目(GJJ12620)；江苏省农产品物理加工重点实验室开放课题(JAPP2010-5)；

详细信息

中图分类号: TS201.2
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出版历程
- 收稿日期: 2012-02-05

Effect of hydrochloric acid hydrolysis on antioxidant property of lotus leaf

摘要

摘要: 在前期发现荷叶具有很强抗氧化活性的基础上,对酸水解其粗提物和有效部位对其抗氧化能力的影响进行了研究。结果表明,盐酸水解后,荷叶粗提物抗氧化能力得到显著提高。粗提物总还原力（OD700）由0.092±0.001提高到0.111±0.001,FRAP（ferric-reducing antioxidant power）法抗氧化能力（OD593）由0.606±0.004提高到0.863±0.001,DPPH（1,1-diphenyl-1-picrylhydrazyl）自由基清除率由29.371%±0.393%提高到90.462%±0.393%,羟自由基清除率从42.456%±2.391%提高到73.196%±0.585%,超氧阴离子清除率由60.328%±1.808%提高到77.330%±6.983%,有效部位总抗氧化力（OD695）由0.540±0.024提高到0.802±0.025。结果证明酸水解是进一步提高荷叶抗氧化能力的有效手段。
- 荷叶; /
- 酸水解; /
- 抗氧化;
Abstract: The effect of hydrochloric acid hydrolysis on antioxidant property of lotus leaf was studied based on that lotus leaf that was proved to possess strong antioxidant capacity.The result showed that the antioxidant capacities of lotus leaf were significantly improved after being hydrolyzed by hydrochloric acid.As for the crude extracts, the reducing power (OD700) was improved from 0.092±0.001 to 0.111±0.001, FRAP values was improved from 0.606±0.004 to 0.863±0.001, DPPH radicals scavenging percentage was improved from 29.371%±0.393% to 90.462%±0.393%, hydroxyl radical scavenging percentage was improved from 42.456%±2.391% to 73.196%±0.585%, superoxide anion radicals scavenging percentage was improved from 60.328%±1.808% to 77.330%±6.983%, respectively.As for the effective fraction, the total antioxidant capacity (OD695) was improved from 0.540±0.024 to 0.802±0.025.These results demonstrated that hydrochloric acid hydrolysis was a effective way to improve the antioxidant activity of lotus leaf.
- lotus leaf;hydrochloric acid hydrolysis;antioxidant activity; /
- undefined /
- undefined

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参考文献(33)

[1]	Gill S S, Tuteja N.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J].Plant Physiology and Biochemistry, 2010, 48 (12) :909-930.
[2]	Reuter S, Gupta S C, Chaturvedi M M, et al.Oxidative stress, inflammation, and cancer:How are they linked?[J].Free Radical Biology and Medicine, 2010, 49 (11) :1603-1616.
[3]	Al-Gubory K H, Fowler P A, Garrel C.The roles of cellular reactive oxygen species, oxidative stress and antioxidants in pregnancy outcomes[J].The International Journal of Biochemistry&Cell Biology, 2010, 42 (10) :1634-1650.
[4]	Huang B, Ban X Q, He J S, et al.Comparative analysis of essential oil components and antioxidant activity of extracts of Nelumbo nucifera from various areas of China[J].Journal of Agricultural and Food Chemistry, 2010, 58 (1) :441-448.
[5]	Deng S G, Deng Z Y, Fan Y W, et al.Isolation and purification of three flavonoid glycosides from the leaves of Nelumbo nucifera (Lotus) by high-speed counter-current chromatography[J].Journal of Chromatography B, 2009, 877 (24) :2487-2492.
[6]	Huang C F, Chen Y W, Yang C Y, et al.Extract of Lotus Leaf (Nelumbo nucifera) and its active constituent catechin with insulin secretagogue activity[J].Journal of Agricultural and Food Chemistry, 2011, 59 (4) :1087-1094.
[7]	Choe J H, Jang A, Lee E S, et al.Oxidative and color stability of cooked ground pork containing lotus leaf (Nelumbo nucifera) and barley leaf (Hordeum vulgare) powder during refrigerated storage[J].Meat Science, 2011, 87 (1) :12-18.
[8]	Wu MJ, Wang LS, Weng CY, et al.Antioxidant activity of methanol extract of the lotus leaf (Nelumbo nucifera Gertn.) [J].American Journal of Chinese Medicine, 2003, 31 (5) :687-698.
[9]	Heo BG.Park YS.Hou WN, et al.In vitro assay on physiological activities of flower and leaf extracts of red lotus[J].Korean Journal of Horticultural Science&Technology, 2008, 26 (3) :331-337.
[10]	Cho E J, Yokozawa T, Rhyu D Y, et al.Study on the inhibitory effects of Korean medicinal plants and their main compounds on the1, 1-diphenyl-2-picrylhydrazyl radical[J].Phytomedicine, 2003 (10) :544-551.
[11]	吕静.荷叶黄酮的体外抗氧化活性研究[J].西藏科技, 2010, 205 (4) :12-15.
[12]	Sultana B, Anwar F, Przybylski R.Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam.trees[J].Food Chemistry, 2007, 104 (3) :1106-1114.
[13]	冯淑环, 殷丽君, 袁蕊, 等.酶法转化糖苷型沙棘叶黄酮生成苷元工艺优化[J].农业机械学报, 2011, 42 (4) :127-132.
[14]	Lin H Y, Kuo Y H, Lin Y L, Chiang W C.Antioxidative effect and active components from leaves of Lotus (Nelumbonucifera) [J].Journal of Agricultral and Food Chemistry, 2009, 57:6623-6629.
[15]	沈建福, 张英, 徐维娅, 等.竹叶黄酮糖苷的水解及其苷元的抗氧化性能研究——II黄酮苷元抗油脂氧化性能的初步评价[J].中国粮油学报, 2001, 16 (4) :14-16.
[16]	刘亚男, 程艳华, 胡福良, 等.蜂胶黄酮苷的酸解及酸解产物抗氧化性能的研究[J].食品科学, 2009, 30 (3) :47-50.
[17]	Jiang S H, Li H Q, Ma H L, et al.Antioxidant activities of selected Chinese medicinal and edible plants[J].International Journal of Food Sciences and Nutrition, 2011, 62 (5) :441-444.
[18]	江慎华, 马海乐, 王昌禄, 等.荷叶活性物质提取工艺及其抗氧化活性的研究[J].农业机械学报, 2010, 41 (7) :141146.
[19]	Bae H, Jayaprakasha G, Jifon J, et al.Extraction efficiency and validation of HPLC method for the flavonoid analysis from peppers[J].Food Chemistry, 2011, 130 (3) :751-758.
[20]	Gu F, Kim J M.Characteristics and antioxidant activity of ultrafiltrated Maillard reaction products from a casein-glucose model system[J].Food Chemistry, 2009, 117 (1) :48-54.
[21]	Jeong C H, Choi G N.Antioxidant activities from the aerial parts of Platycodon grandiflorum[J].Food Chemistry, 2010, 118 (2) :278-282.
[22]	Yang G M, Wang D.Anti-inflammatory and Antioxidant Activities of Oxytropis falcata Fractions and Its Possible Antiinflammatory Mechanism[J].Chinese Journal of Natural Medicines, 2010, 8 (4) :285-292.
[23]	Duan X, Jiang Y, Su X, et al.Antioxidant properties of anthocyanins extracted from litchi (Litchi chinenesis Sonn.) fruit pericarp tissues in relation to their role in the pericarp browning[J].Food Chemistry, 2007, 101 (4) :1365-1371.
[24]	Pan Y, He C, Wang H, et al.Antioxidant activity of microwaveassisted extract of Buddleia officinalis and its major activecomponent[J].Food Chemistry, 2010, 121 (2) :497-502.
[25]	Ammar R B, W Bhouri.Antioxidant and free radical scavenging properties of three flavonoids isolated from the leaves of Rhamnus alaternus L. (Rhamnaceae) :A structure-activity relationship study[J].Food Chemistry, 2009, 116 (1) :258-264.
[26]	Guo T, Wei L, Sun J, et al.Antioxidant activities of extract and fractions from Tuber indicum Cooke&Massee[J].Food Chemistry, 2011, 127 (4) :1634-1640.
[27]	Chen R, Liu Z, Zhao J, et al.Antioxidant and immunobilological activity of a water-soluble polysaccharide fractions purified from Acanthopanax Senticosu[J].Food Chemistry, 2011, 127 (2) :434440.
[28]	Blokhina O, Virolainen E, Fagerstedt K V.Antioxidants, oxidative damage and oxygen deprivation stress:A review[J].Annals of Botany, 2003, 91 (2) :179-194.
[29]	Halliwell B.Reactive oxygen species in living systems:source, biochemistry, and role in human disease[J].The American Journal of Medicine, 1991, 91 (3) :S14-S22.
[30]	Korycka-Dahl M, Richardson T.Photogeneration of superoxide anion in serum of bovine milk and in model systems containing riboflavin and amino acids[J].Journal of Dairy Science, 1978, 61 (4) :400-407.
[31]	Heim K E, Tagliaferro A R, Bobilya D J.Flavonoid antioxidants:chemistry, metabolism and structure-activity relationships[J].The Journal of Nutritional Biochemistry, 2002, 13 (10) :572-584.
[32]	Lai H H, Yen G C.Inhibitory effect of isoflavones on peroxynitrite-mediated low-density lipoprotein oxidation[J].Biosci Biotechnol Biochem, 2002, 66 (1) :22-28.
[33]	Plumb G W, Price K R, Williamson G.Antioxidant properties of flavonol glycosides from green beans[J].Redox Report, 1999, 4 (3) :123-127.

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