Determination of 36 Veterinary Drug Residues in Cooked Meat by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry

Fei ZHANG; Yuan WANG; Xiaoning MA; Jinlei ZHANG; Jingting GONG; Mingxin FU

doi:10.13386/j.issn1002-0306.2022080155

Volume 44 Issue 13

Jun. 2023

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Science and Technology of Food Industry > 2023 > 44(13): 340-348. > DOI: 10.13386/j.issn1002-0306.2022080155

ZHANG Fei, WANG Yuan, MA Xiaoning, et al. Determination of 36 Veterinary Drug Residues in Cooked Meat by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2023, 44(13): 340−348. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080155.

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Determination of 36 Veterinary Drug Residues in Cooked Meat by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry

Analysis and Testing Center, Xinjiang Academy of Agricultural Reclamation Science, Shihezi 832000, China

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Received Date: August 16, 2022
Available Online: May 07, 2023

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Abstract

Abstract

A method was developed for the determination of amantadine sulfonamides, quinolones and chloramphenicols in cooked meat. The samples were extracted with acetonitrile-water mixtures (V:V=8:2), purified with PRiME HLB solid phase extraction column. 36 veterinary drugs were determined by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with an electrospray ion source (ESI±) in positive and negative ion switching scanning mode. The results showed that the 36 veterinary drugs showed a good linear relationship in the concentration range of 0.5~20.0 ng/mL (R²>0.999). The limits of detection were 0.10 μg/kg and the limits of quantitation were 0.33 μg/kg. The average recovery was 70.8%~106.9% in the range of high, medium and low concentrations (0.5, 1.0 and 5.0 μg/kg). The relative standard deviations (RSD, n=6) was 0.2%~6.2%. The method is simple, sensitive and accurate, and could be used for the determination of 36 veterinary drug residues in cooked meat.

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[1]	段玉林, 陈栎岩, 宁方尧. 肉制品中重金属及兽药残留现状研究[J]. 食品安全质量检测学报,2021,12(8):3008−3015. [DUAN Y L, CHEN L Y, NING F Y. Study on residues of heavy metals and veterinary drugs in meat products[J]. Journal of Food Safety & Quality,2021,12(8):3008−3015. DUAN Y L, CHEN L Y, NING F Y. Study on residues of heavy metals and veterinary drugs in meat products[J]. Journal of Food Safety & Quality, 2021, 12(8): 3008-3015.
[2]	张秋霞, 张涛, 韩瑾瑾. 兽药残留的种类及检测方法[J]. 畜禽业,2019,30(9):33. [ZHANG Q X, ZHANG T, HAN J J. Types and detection methods of veterinary drug residues[J]. Livestock and Poultry Industry,2019,30(9):33. ZHANG Q X, ZHANG T, HAN J J. Types and detection methods of veterinary drug residues[J]. Livestock and Poultry Industry, 2019, 30(9): 33.
[3]	马芳. 兽药残留种类及检测方法[J]. 畜牧兽医科学,2020,15(5):30−31. [MA F. Types and detection methods of veterinary drug residues[J]. Graziery Veterinary Sciences,2020,15(5):30−31. MA F. Types and detection methods of veterinary drug residues[J]. Graziery Veterinary Sciences, 2020, 15(5): 30-31.
[4]	ZHANG H, CHEN Q, NIU B. Risk assessment of veterinary drug residues in meat products[J]. Current Drug Metabolism,2020,21:779−789. doi: 10.2174/1389200221999200820164650
[5]	高金芳, 岳婷婷, 赵祎, 等. 兽药多残留样品前处理技术研究进展[J]. 畜牧兽医学报,2017,48(1):8−22. [GAO J F, YUE T T, ZHAO Y, et al. Research progress on pretreatment of multi-residue samples of veterinary drugs[J]. Chinese Journal of Animal and Veterinary Sciences,2017,48(1):8−22. GAO J F, YUE T T, ZHAO Y, et al. Research progress on pretreatment of multi-residue samples of veterinary drugs[J]. Chinese Journal of Animal and Veterinary Sciences, 2017, 48(1): 8-22.
[6]	蒋磊, 苏绍辉. 动物性食品中兽药残留的危害及预防措施[J]. 现代畜牧科学,2021,5:10−11. [JIANG L, SU S H. Hazards and preventive of veterinary drug residues in animal food[J]. Modern Animal Husbandry Science,2021,5:10−11. JIANG L, SU S H. Hazards and preventive of veterinary drug residues in animal food[J]. Modern Animal Husbandry Science, 2021, 05: 10-11.
[7]	阿力腾才斯克, 萨仁高娃. 动物性食品中兽药残留危害及成因[J]. 畜牧兽医科学,2020,14(14):156−157. [ALIT Z, SAREN G. Effects of residues drugs on animal food[J]. Graziery Veterinary Sciences,2020,14(14):156−157. ALIT Z, SAREN G. Effects of residues drugs on animal food[J]. Graziery Veterinary Sciences, 2020, 14: 156-157.
[8]	郭莉. 畜禽产品兽药残留危害的现状与分析[J]. 畜牧兽医科技信息,2019,43(3):130−134. [GUO L. Present situation and analysis of veterinary drug residue harm in livestock and poultry products[J]. Chinese Journal of Animal Husbandry and Veterinary Medicine,2019,43(3):130−134. GUO L. Present situation and analysis of veterinary drug residue harm in livestock and poultry products[J]. Chinese Journal of Animal Husbandry and Veterinary Medicine, 2019, 43(3): 130-134.
[9]	张根生, 王军茹, 岳晓霞, 等. 卤肉制品加工过程中风味物质形成机理和变化研究过程[J]. 中国调味品,2021,11:195−200. [ZHANG G S, WANG J R, YUE X X, et al. Study on the formation mechanism and change of flavor substances in the processing of halogen meat products[J]. China Condiment,2021,11:195−200. ZHANG G S, WANG J R, YUE X X, et al. Study on the formation mechanism and change of flavor substances in the processing of halogen meat products[J]. China Condiment, 2021, 11: 195-200.
[10]	朱平, 张秀宇, 何涛, 等. 2016-2019年国家肉制品监督检验结果分析[J]. 食品安全检测学报,2020,11(16):5594−5600. [ZHU P, ZHANG X Y, He T, et al. Analysis of national meat product supervision on inspection results from 2016 to 2019[J]. Journal of Food Safety & Quality,2020,11(16):5594−5600. ZHU P, ZHANG X Y, He T, et al. Analysis of national meat product supervision on inspection results from 2016 to 2019[J]. Journal of Food Safety & Quality, 2020, 11(16): 5594-5600.
[11]	YANG Y, YE Y F, PAN D D, et al. Meta bonomics profiling of marinated meat in soy sauce during processing[J]. Science of Food and Agriculture,2017,98(4):1325−1331.
[12]	李燕君, 谭梅, 岳秀英, 等. 牛奶中氟喹诺酮类药物残留同步快速检测的酶联免疫吸附法研究[J]. 中国兽药杂志,2020,54(1):46−52. [LI Y J, TAN M, YUE X Y, et al. Simultaneous and rapid detection of fluoroquinolone residues in milk by enzyme-linked immunosorbent assay[J]. Chinese Journal of Veterinary Drug,2020,54(1):46−52. LI Y J, TAN M, YUE X Y, et al. Simultaneous and rapid detection of fluoroquinolone residues in milk by enzyme-linked immunosorbent assay[J]. Chinese Journal of Veterinary Drug, 2020, 54(1): 46-52.
[13]	李然, 李泽佳, 杨金易, 等. 酶联免疫法检测动物组织及尿液中氟苯尼考与甲砜霉素的残留[J]. 分析化学,2018,46(8):1321−1328. [LI R, LI Z J, YANG J Y, et al. The residues of florfenicol and thiamphenicol in animal tissue were detected by enzyme-linked immunosorbent assay[J]. Chinese Journal of Analytical Chemistry,2018,46(8):1321−1328. doi: 10.1016/S1872-2040(18)61104-1 LI R, LI Z J, YANG J Y, et al. The residues of florfenicol and thiamphenicol in animal tissue were detected by enzyme-linked immunosorbent assay[J]. Chinese Journal of Analytical Chemistry, 2018, 46(8): 1321-1328. doi: 10.1016/S1872-2040(18)61104-1
[14]	WANG G, ZHANG H C, LIU J, et al. A receptor-based chemiluminescence enzyme linked immunosorbent assay for determination of tetracyclines in milk[J]. Analytical Biochemistry,2019,40(46):564−565.
[15]	滕爽, 吕青骎, 沈娟, 等. 超高效液相色谱法检测猪肉及其制品中的磺胺类及氟喹诺酮类兽药残留[J]. 食品与发酵工业,2018,44(7):263−268. [TENG S, LÜ Q Q, SHEN J, et al. Determination of sulfonamides and fluoroquinolones in pork and its products ultra-high performance liquid chromatography[J]. Food and Fermentation Industries,2018,44(7):263−268. TENG S, LÜ Q Q, SHEN J, et al. Determination of sulfonamides and fluoroquinolones in pork and its products ultra-high performance liquid chromatography[J]. Food and Fermentation Industries, 2018, 44(7): 263-268.
[16]	AGADELLS E, TARTAGLIA A, LOCATELLI M, et al. Mixed-mode fabric phase sorptive extraction of multiple tetracycline residues from milk samples prior to high performance liquid chromatography-ultraviolet analysis[J]. Micrechemical Journal,2020,159:105437. doi: 10.1016/j.microc.2020.105437
[17]	符靖雯, 黄子敬, 陈孟君, 等. 气相色谱-电子捕获检测器快速测定水产品中多种农药及兽药残留[J]. 理化检验-化学分册,2018,54(9):1015−1019. [FU J W, HUANG Z J, CHEN M J. et al. Rapid determination of pesticide and veterinary drug residues in aquatic products by gas chromatography-electron capture detector[J]. PhyTestChem Anal Part B,2018,54(9):1015−1019. FU J W, HUANG Z J, CHEN M J. et al. Rapid determination of pesticide and veterinary drug residues in aquatic products by gas chromatography-electron capture detector[J]. PhyTestChem Anal Part B, 2018, 54(9): 1015-1019.
[18]	张春辉. 气相色谱质谱法测定动物源性食品中氯霉素[J]. 分析实验室,2020,39(6):726−730. [ZHANG C H. Determination of chloramphenicol in food of animal origin by gas chromatography-mass spectrometry[J]. Chinese Journal of Analysis Laboratory,2020,39(6):726−730. ZHANG C H. Determination of chloramphenicol in food of animal origin by gas chromatography-mass spectrometry[J]. Chinese Journal of Analysis Laboratory, 2020, 39(6): 726-730.
[19]	裴雯, 韩萍, 王杰, 等. 基于UHPLC-Q-TOF/MS法同时检测猪肉中主要抗生素类兽药残留[J]. 食品工业科技,2022,43(10):298−304. [PEI W, HAN P, WANG J, et al. UHPLC-Q-TOF/MS was uesd to detect the residues of major antibiotics in pork[J]. Science and Technology of Food Industry,2022,43(10):298−304. PEI W, HAN P, WANG J, et al. UHPLC-Q-TOF/MS was uesd to detect the residues of major antibiotics in pork[J]. Science and Technology of Food Industry, 2022, 43(10): 298-304.
[20]	REZENDE D R, FILHO N L, ROCHA G L, et al. Simultaneous determination of chloramphenicol and florfenicol in liquid milk, milk powder and bovine muscle by LC-MS/MS[J]. Food Additives & Contaminants:Part A,2012,45:559−570.
[21]	吴明, 徐飞. 液相色谱-串联质谱法测定鸡蛋中的喹诺酮类和四环素类抗生素[J]. 食品工业科技,2019,40(3):249−254. [WU M, XU F. Determination of quinolones and tetracycline antibiotics in eggs by liquid chromatography tandem mass spectrometry[J]. Science and Technology of Food Industry,2019,40(3):249−254. WU M, XU F. Determination of quinolones and tetracycline antibiotics in eggs by liquid chromatography tandem mass spectrometry[J]. Science and Technology of Food Industry, 2019, 40(3): 249-254.
[22]	LI J, REN X L, DIAO Y Y, et al. Multiclass analysis of 25 veterinary drugs in milk by ultra-high performance liquid chromatography-tandem mass spectrometry[J]. Food Chemistry,2018,257:259−264. doi: 10.1016/j.foodchem.2018.02.144
[23]	马俊美, 何亮娜, 孙磊, 等. 在线固相萃取-液相色谱-串联质谱法测定鸡肉和鸡蛋中5种抗病毒类药物残留量[J]. 食品科学,2021,42(10):305−310. [MA J M, HE L N, SUN L, et al. Determination of residues of 5 antiviral drugs in chicken and egg liquid chromatography tandem mass spectrometry[J]. Food Science,2021,42(10):305−310. MA J M, HE L N, SUN L, et al. Determination of residues of 5 antiviral drugs in chicken and egg liquid chromatography tandem mass spectrometry[J]. Food Science, 2021, 42(10): 305-310.
[24]	蔡雪, 周川, 杨淑芬, 等. 大米中黄曲霉毒素B₁酶联免疫法与液相色谱-柱后衍生法的比对探讨[J]. 分析检测,2019,11:186−189. [CAI X, ZHOU C, YANG S F, et al. Comparison of aflatoxin B₁ in rice by enzyme-linked immunoassay and liquid chromatography-post column derivation[J]. Journal of Instrumental Analysis,2019,11:186−189. CAI X, ZHOU C, YANG S F, et al. Comparison of aflatoxin B₁ in rice by enzyme-linked immunoassay and liquid chromatography-post column derivation[J]. Journal of Instrumental Analysis, 2019, 11: 186-189.
[25]	邓高琼, 陈亨业, 刘瑞, 等. 气相色谱-质谱联用技术在食药检测中的应用与发展[J]. 化学试剂,2021,43(5):555−562. [DENG G Q, CHEN H Y, LIU R, et al. Application and development of gas chromatography-mass spectrometry in food and drug detection[J]. Chemical Reagents,2021,43(5):555−562. DENG G Q, CHEN H Y, LIU R, et al. Application and development of gas chromatography-mass spectrometry in food and drug detection[J]. Chemical Reagents, 2021, 43(5): 555-562.
[26]	AN H, PARRALES L, WANG K, et al. Quantitative analysis of nitrofuran metabolites and chloramphenicol in shrimp using acetonitrile extraction and liquid chromatograph-tandem mass spectrometric detection: a single laboratory validation[J]. Journal of AOAC International,2019,98(3):602−608.
[27]	GUO P, WAN J C, ZHAN C R, et al. A simplified sample pretreatment for the rapid determination of 22 β-agonist residues in swine muscle and liver tissues by ultra-high-performance liquid chromatography tandem mass spectrometry[J]. Journal of Chromatography B,2018,1096:122−134. doi: 10.1016/j.jchromb.2018.07.038
[28]	张敏, 王鸽, 马晓冲. 等. 高效液相色谱-串联质谱法测定牛乳中6中兽药残留[J]. 食品工业科技,2022,43(5):255−260. [ZHANG M, WANG G, MA X C, et al. Determination of 6 veterinary drug residues in milk by high performance liquid chromatography-tandem mass spectrometry[J]. Science and Technology of Food Industry,2022,43(5):255−260. ZHANG M, WANG G, MA X C, et al. Determination of 6 veterinary drug residues in milk by high performance liquid chromatography-tandem mass spectrometry[J]. Science and Technology of Food Industry, 2022, 43(5): 255-260.
[29]	陈兴连, 林涛, 刘兴勇, 等. 超高效液相色谱-串联质谱法快速测定鱼和虾中多类禁、限用兽药残留[J]. 色谱,2020,47(8):538−546. [CHEN X L, LIN T, LIU X Y, et al. Rapid determination of multiple and restricted veterinary drug residues in fish and shrimp by ultra high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography,2020,47(8):538−546. CHEN X L, LIN T, LIU X Y, et al. Rapid determination of multiple and restricted veterinary drug residues in fish and shrimp by ultra high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2020, 47(8): 538-546.
[30]	姜文华, 韦立毅, 邱晋, 等. 超高效液相色谱-串联质谱测定猪肉中93种兽药和违禁化合物[J]. 实验技术及其应用,2020,47(8):1477−1483. [JIANG W H, WEI L Y, QIU J, et al. Determination of 93 veterinary drugsand prohibited chemicals in pork by ultra high performance liquid chromatography-tandem mass spectrometry[J]. Modem Preventive Medicine,2020,47(8):1477−1483. JIANG W H, WEI L Y, QIU J, et al. Determination of 93 veterinary drugsand prohibited chemicals in pork by ultra high performance liquid chromatography-tandem mass spectrometry[J]. Modem Preventive Medicine, 2020, 47(8): 1477-1483.
[31]	李永琴, 卜宁霞, 陈娟, 等. HPLC-MS/MS法同时测定牛肉中80中兽药及其代谢物[J]. 食品科学科技学报,2022,40(1):140−149. [LI Y Q, BO N X, CHEN J, et al. Determination of 80 veterinary drug residues and their metabolites in beef by HPLC-MS/MS[J]. Journal of Food Science and Technology,2022,40(1):140−149. LIYQ, BONX, CHENJ, et al. Determination of 80 veterinary drug residues and their metabolites in beef by HPLC-MS/MS[J], Journal of Food Science and Technology, 2022, 40(1): 140-149.

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