ZHAO Liming, GUO Xuyao, MAO Yingmin, et al. Optimization of Extraction Process and Antioxidant Activity of Polysaccharide from Panax quinquefolium Fruit by Response Surface Methodology[J]. Science and Technology of Food Industry, 2023, 44(13): 160−166. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070318.
Citation: ZHAO Liming, GUO Xuyao, MAO Yingmin, et al. Optimization of Extraction Process and Antioxidant Activity of Polysaccharide from Panax quinquefolium Fruit by Response Surface Methodology[J]. Science and Technology of Food Industry, 2023, 44(13): 160−166. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070318.

Optimization of Extraction Process and Antioxidant Activity of Polysaccharide from Panax quinquefolium Fruit by Response Surface Methodology

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  • Received Date: August 01, 2022
  • Available Online: May 07, 2023
  • Objective: For the extraction of polysaccharides from American ginseng fruits, the extraction process was optimized in combination with response surface methodology, and to investigate whether polysaccharides from American ginseng fruits had antioxidant activity in vitro. Methods: In this study, polysaccharides were extracted from fresh American ginseng fruits by water extraction and alcohol precipitation method. The extraction process was optimized using single factor tests as well as response surface methodology. In vitro antioxidant activity studies of polysaccharides from American ginseng fruits were conducted from three aspects: DPPH radical scavenging rate, hydroxyl radical scavenging rate as well as reducing power. Results: The optimal process parameters were as follows: the extraction time was 2.5 h, the concentration of ethanol was 80%, the feed to liquid ratio of 1:16 g/mL, and the measured average value of polysaccharide yield was 29.47%±0.65%, which was comparable to the model predicted value. In vitro antioxidant activities of polysaccharides from American ginseng fruits were examined in the following three aspects. When the concentration of polysaccharides was 3.4 mg/mL, the DPPH radical scavenging rate was 75.14%±0.65%, and IC50 value was 0.71 mg/mL. When the concentration of polysaccharide was 3.4 mg/mL, the scavenging rate against hydroxyl radicals could reach 71.82%±1.43%, and IC50 value was 0.87 mg/mL. When the concentration of polysaccharides was 1.0 mg/mL, the total reducing power reached 0.730, and its in vitro antioxidant capacity was enhanced with increasing concentrations of polysaccharides from American ginseng fruits. Conclusion: The experimental results of antioxidant activities illustrated that polysaccharides from American ginseng fruits had better antioxidant activities. This study may provide a theoretical basis for further development and utilization of polysaccharides from American ginseng fruits, and ensure that traditional Chinese medicine resources can be fully utilized.
  • [1]
    LIU Chengzhi, CHEN Weiwang, MEI Xia, et al. Dendrobium officinale Kimura et Migo and American ginseng mixture: A Chinese herbal formulation for gut microbiota modulation[J]. Chinese Journal of Natural Medicines,2020,18(6):446−459. doi: 10.1016/S1875-5364(20)30052-2
    [2]
    王秋, 刘莉, 何慧楠, 等. 不同生长年限西洋参不同部位氨基酸含量比较研究[J]. 中华中医药杂志,2017,32(8):3692−3695. [WANG Qiu, LIU Li, HE Huinan, et al. Comparative study of amino acid content of American ginseng[J]. China Journal of Traditional Chinese Medicine and Pharmacy,2017,32(8):3692−3695.

    WANG Qiu, LIU Li, HE Huinan, et al. Comparative study of amino acid content of American ginseng[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2017, 32(8): 3692-3695.
    [3]
    QI Junshan, MA Liguo, ZHANG Bo, et al. First report of root and crown rot of American ginseng caused by Pythium spinosum in China[J]. Plant Disease, 2020.
    [4]
    DENNIE VC A, FCIC. Study on north American ginseng[J]. Focus on Alternative and Complementary Therapies,2006,11(2):178. doi: 10.1211/fact.11.2.0079
    [5]
    邓沂. 夏季清补, 了解一下西洋参[J]. 中医健康养生,2021,7(6):39−41. [DENG Qi. Summer cleaning, learn about American ginseng[J]. TCM Healthy Life-Nurturing,2021,7(6):39−41.

    DENG Qi. Summer cleaning, learn about American ginseng[J]. TCM Healthy Life-Nurturing, 2021, 7(6): 39-41.
    [6]
    李乐, 余慧, 付书正, 等. 西洋参果化学成分及其生物活性研究进展[J]. 中南药学,2021,19(5):931−936. [LI Le, YU Hui, FU Zhengshu, et al. Research progress on chemical constituents and biological activities of American ginseng fruit[J]. Central South Pharmacy,2021,19(5):931−936.

    LI Le, YU Hui, FU Zhengshu, et al. Research progress on chemical constituents and biological activities of American ginseng fruit[J]. Central South Pharmacy, 2021, 19(5): 931-936.
    [7]
    LIU Lu, XU Furong, WANG Yuanzhong. Traditional uses, chemical diversity and biological activities of Panax L. (Araliaceae): A review[J]. Journal of Ethnopharmacology,2020,263:112792. doi: 10.1016/j.jep.2020.112792
    [8]
    钟运香, 袁娇, 刘丰惠, 等. 西洋参化学成分、药理作用及质量控制研究进展[J]. 中国中医药现代远程教育,2020,18(7):130−133. [ZHONG Yunxiang, YUAN Jiao, LIU Huifeng, et al. Research progress on chemical constituents, pharmacological effects and quality control of American ginseng[J]. Chinese Medicine Modern Distance Education of China,2020,18(7):130−133. doi: 10.3969/j.issn.1672-2779.2020.07.053

    ZHONG Yunxiang, YUAN Jiao, LIU Huifeng, et al. Research progress on chemical constituents, pharmacological effects and quality control of American ginseng[J]. Chinese Medicine Modern Distance Education of China, 2020, 18(7): 130-133. doi: 10.3969/j.issn.1672-2779.2020.07.053
    [9]
    XIE J T, WANG C Z, NI M, et al. American ginseng berry juice intake reduces blood glucose and body weight in ob/ob mice[J]. Journal of Food Science,2007,72(8):S590−S594. doi: 10.1111/j.1750-3841.2007.00481.x
    [10]
    毛英民, 于文杰, 李佳奇, 等. HPLC 结合化学计量法对西洋参不同部位中人参皂苷的差异分析[J]. 中华中医药学刊,2023,41(1):8. [MAO Yingmin, YU Wenjie, LI Jiaqi, et al. Difference analysis of ginsenosides in different parts of American ginseng by HPLC combined with chemometrics[J]. Chinese Archives of Traditional Chinese Medicine,2023,41(1):8.

    MAO Yingmin, YU Wenjie, LI Jiaqi, et al. Difference analysis of ginsenosides in different parts of American ginseng by HPLC combined with chemometrics[J]. Chinese Archives of Traditional Chinese Medicine, 2023, 41(1): 8.
    [11]
    曾俊. 西洋参多糖的提取分离、结构表征及抗氧化活性研究[D]. 哈尔滨: 哈尔滨工业大学, 2022.

    ZENG Jun. Study on extraction, separation, structure characterization and antioxidant activity of polysaccharides from American ginseng[D]. Harbin: Harbin Institute of Technology, 2022.
    [12]
    贾琦琦. 西洋参多糖提取、性质及口服液制备[D]. 泰安: 山东农业大学, 2021.

    JIA Qiqi. Extraction, properties and oral liquid preparation of American ginseng polysaccharide[D]. Taian: Shandong Agricultural University, 2021.
    [13]
    ZHOU Rongrong, HUANG Jiahua, HE Dan, et al. Green and efficient extraction of polysaccharide and ginsenoside from American ginseng (Panax quinquefolius L.) by deep eutectic solvent extraction and aqueous two-phase system[J]. Molecules,2022,27(10):3132. doi: 10.3390/molecules27103132
    [14]
    李珊珊, 祁玉丽, 曲迪, 等. 西洋参果多糖的纯化及DPPH自由基清除活性研究[J]. 特产研究,2019,41(1):1−4. [LI Shanshan, QI Yuli, QU Di, et al. Study on purification and DPPH free radical scavenging activity of polysaccharide from American ginseng fruit[J]. Special Wild Economic Animal and Plant Research,2019,41(1):1−4. doi: 10.16720/j.cnki.tcyj.2019.01.001

    LI Shanshan, QI Yuli, QU Di, et al. Study on purification and DPPH free radical scavenging activity of polysaccharide from american ginseng fruit[J]. Special Wild Economic Animal and Plant Research, 2019, 41(1): 1-4. doi: 10.16720/j.cnki.tcyj.2019.01.001
    [15]
    李思齐, 余学英, 何芳. Box-Behnken效应面法优化粗榧叶总黄酮提取工艺及其体外活性研究[J]. 中国现代中药,2018,21(1):1270−1275. [LI Siqi, YU Xueying, HE Fang. Optimization of extraction process and in vitro activity of total flavonoids from Torreya grandis leaves by Box-Behnken response surface method[J]. Modern Chinese Medicine,2018,21(1):1270−1275. doi: 10.13313/j.issn.1673-4890.20200920002

    LI Siqi, YU Xueying, HE Fang. Optimization of extraction process and in vitro activity of total flavonoids from Torreya grandis leaves by Box-Behnken response surface method[J]. Modern Chinese Medicine, 2018, 21(1): 1270-1275. doi: 10.13313/j.issn.1673-4890.20200920002
    [16]
    YANG Chengjung, LIN Mei, CHEN Jahau. A multi-disciplinary optimization approach to eco-friendly design using the response surface method[J]. Applied Sciences,2022,6(12):3002.
    [17]
    王雪飞, 张华, 王振宇. 响应面法优化黄柏花粉多糖的脱蛋白工艺及其抗氧化活性[J]. 食品工业,2018,39(4):7−12. [WANG Xuefei, ZHANG Hua, WANG Zhenyu. Optimization of deproteinization process of Phellodendron pollen polysaccharide by response surface methodology and its antioxidant activity[J]. The Food Industry,2018,39(4):7−12.

    WANG Xuefei, ZHANG Hua, WANG Zhenyu. Optimization of deproteinization process of Phellodendron pollen polysaccharide by response surface methodology and its antioxidant activity[J]. The Food Industry, 2018, 39(4): 7-12.
    [18]
    陈江艳, 王维滔, 董益阳, 等. 响应面优化蒲公英橡胶草菊糖提取工艺及其MALDI-TOF MS分析[J]. 食品工业科技,2022,43(1):205−212. [CHEN Jiangyan, WANG Weitao, DONG Yiyang, et al. Response surface optimization of dandelion rubber grass inulin extraction process and MALDI-TOF MS analysis[J]. Science and Technology of Food Industry,2022,43(1):205−212.

    CHEN Jiangyan, WANG Weitao, DONG Yiyang, et al. Response surface optimization of dandelion rubber grass inulin extraction process and MALDI-TOF MS analysis[J]. Science and Technology of Food Industry, 2022, 43(1): 205-212.
    [19]
    李英娜, 潘美彤, 苑广信, 等. 响应面法优化复方北冬虫夏草颗粒多糖提取工艺的研究[J]. 食品研究与开发,2020,41(2):61−66. [LI Yingna, PAN Meitong, YAUN Guangxin, et al. Study on optimization of extraction process of polysaccharide from compound north cordyceps granules by response surface methodology[J]. Food Research and Development,2020,41(2):61−66. doi: 10.12161/j.issn.1005-6521.2020.02.011

    LI Yingna, PAN Meitong, YAUN Guangxin, et al. Study on optimization of extraction process of polysaccharide from compound north cordyceps granules by response surface methodology[J]. Food Research and Development, 2020, 41(2): 61-66. doi: 10.12161/j.issn.1005-6521.2020.02.011
    [20]
    蔡群虎, 刘石磊, 袁敏惠, 等. 三七多糖含量测定方法比较[J]. 云南中医中药杂志,2020,41(8):59−62. [CAI Qunhu, LIU Shilei, YUAN Minhui, et al. Comparison of determination methods of polysaccharide content in Panax notoginseng[J]. Yunnan Journal of Traditional Chinese Medicine and Materia Medica,2020,41(8):59−62. doi: 10.3969/j.issn.1007-2349.2020.08.020

    CAI Qunhu, LIU Shilei, YUAN Minhui, et al. Comparison of determination methods of polysaccharide content in Panax notoginseng[J]. Yunnan Journal of Traditional Chinese Medicine and Materia Medica, 2020, 41(8): 59-62. doi: 10.3969/j.issn.1007-2349.2020.08.020
    [21]
    刘佳妮, 王硕, 白米雪, 等. 响应面法优化丹参药渣多糖提取工艺及其抗氧化活性研究[J]. 时珍国医国药,2017,28(2):454−457. [LIU Jiani, WANG Shuo, BAI Mixue, et al. Optimization of extraction process of polysaccharide from salvia root residue by response surface methodology and its antioxidant activity[J]. Lishizhen Medicine and Materia Medica Research,2017,28(2):454−457.

    LIU Jiani, WANG Shuo, BAI Mixue, et al. Optimization of extraction process of polysaccharide from salvia root residue by response surface methodology and its antioxidant activity[J]. Lishizhen Medicine and Materia Medica Research, 2017, 28(2): 454-457.
    [22]
    SHEN S G, JIA S R, WU Y K, et al. Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc flagelliforme[J]. Carbohydrate Polymers,2018,198:426−433. doi: 10.1016/j.carbpol.2018.06.111
    [23]
    胡晓, 于娇, 陈胜军, 等. 末水坛紫菜蛋白源抗氧化肽的制备、分离纯化与体外抗氧化活性[J]. 食品科学,2020,41(16):37−44. [HU Xiao, YU Jiao, CHEN Shengjun, et al. Preparation, purification and antioxidant activity in vitro of antioxidant peptides from Porphyra haitanensis protein[J]. Food Science,2020,41(16):37−44. doi: 10.7506/spkx1002-6630-20190822-231

    HU Xiao, YU Jiao, CHEN Shengjun, et al. Preparation, purification and antioxidant activity in vitro of antioxidant peptides from Porphyra haitanensis protein[J]. Food Science, 2020, 41(16): 37-44. doi: 10.7506/spkx1002-6630-20190822-231
    [24]
    韩东. 黑果腺肋花楸多糖提取纯化与体外抗氧化活性研究[D]. 天津: 天津商业大学, 2022.

    HAN Dong. Purification and antioxidant activity in vitro of polysaccharides from Aronia melanocarpa[D]. Tianjin: Tianjin University of Commerce, 2022.
    [25]
    万茜淋, 焦丽丽, 马林, 等. 响应面法优化人参花多糖的提取工艺[J]. 长春理工大学学报(自然科学版),2015,38(6):154−158, 132. [WAN Qianlin, JIAO Lili, MA Lin, et al. Optimization of polysaccharides from flowers of Panax ginseng C. A. Meyer using response surface methodology[J]. Journal of Changchun University of Science and Technology(Natural Science Edition),2015,38(6):154−158, 132. doi: 10.3969/j.issn.1672-9870.2015.06.036

    WAN Qianlin, JIAO Lili, MA Lin, et al. Optimization of polysaccharides from flowers of panax ginseng C. A. Meyer using response surface methodology[J]. Journal of Changchun University of Science and Technology(Natural Science Edition), 2015, 38(6): 154-158, 132. doi: 10.3969/j.issn.1672-9870.2015.06.036
    [26]
    陈军辉, 谢明勇, 易秀琴, 等. 西洋参多糖提取新工艺研究[J]. 时珍国医国药,2005(10):977−979. [CHEN Junhui, XIE Mingyong, YI Xiuqin, et al. Study on new technological process for extraction of polysaccharide from Panax quinquef olium L[J]. Lishizhen Medicine and Materia Medica Research,2005(10):977−979. doi: 10.3969/j.issn.1008-0805.2005.10.032

    CHEN Junhui, XIE Mingyong, YI Xiuqin, et al. Study on new technological process for extraction of polysaccharide from Panax quinquef olium L[J]. Lishizhen Medicine and Materia Medica Research, 2005, (10): 977-979. doi: 10.3969/j.issn.1008-0805.2005.10.032
    [27]
    王蔚新, 张俊, 占剑峰, 等. 福白菊胎菊与朵菊粗多糖体外抗氧化活性分析[J]. 黄冈师范学院学报,2020,40(6):9−13. [WANG Weixin, ZHANG Jun, ZHAN Jianfeng, et al. Analysis of antioxidant activity in vitro of Fubaiju fetal chrysanthemum and chrysanthemum crude polysaccharide[J]. Journal of Huanggang Normal University,2020,40(6):9−13. doi: 10.3969/j.issn.2096-7020.2020.06.02

    WANG Weixin, ZHANG Jun, ZHAN Jianfeng, et al. Analysis of antioxidant activity in vitro of Fubaiju fetal chrysanthemum and chrysanthemum crude polysaccharide[J]. Journal of Huanggang Normal University, 2020, 40(6): 9-13. doi: 10.3969/j.issn.2096-7020.2020.06.02
    [28]
    李珊, 梁俭, 冯彬, 等. 响应面法优化超声波辅助提取山竹果皮多糖的工艺及其体外抗氧活性研究[J]. 食品研究与开发,2020,41(10):103−110. [LI Shan, LIANG Jian, FENG Bin, et al. Response surface methodology promoted ultrasound-assisted extraction of polysaccharides from mangosteen peels and evaluation of antioxidant activity in vitro[J]. Food Research and Development,2020,41(10):103−110. doi: 10.12161/j.issn.1005-6521.2020.10.018

    LI Shan, LIANG Jian, FENG Bin, et al. Response surface methodology promoted ultrasound-assisted extraction of polysaccharides from mangosteen peels and evaluation of antioxidant activity in vitro[J]. Food Research and Development, 2020, 41(10): 103-110. doi: 10.12161/j.issn.1005-6521.2020.10.018
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