Response Surface Methodology to Optimize the Extraction Process of Rhodiola rosea Polyphenols and Its Inhibitory Activity of α-Glucosidase in Vitro

REN Weihe; LUO Longlong; WANG Liping; GAO Caiwen; YU Jinfeng; Peer Mohamed Abdul; DING Gongtao; CHEN Shien

doi:10.13386/j.issn1002-0306.2021070093

Volume 43 Issue 6

Mar. 2022

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Science and Technology of Food Industry > 2022 > 43(6): 213-220. > DOI: 10.13386/j.issn1002-0306.2021070093

REN Weihe, LUO Longlong, WANG Liping, et al. Response Surface Methodology to Optimize the Extraction Process of Rhodiola rosea Polyphenols and Its Inhibitory Activity of α-Glucosidase in Vitro[J]. Science and Technology of Food Industry, 2022, 43(6): 213−220. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021070093.

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Response Surface Methodology to Optimize the Extraction Process of Rhodiola rosea Polyphenols and Its Inhibitory Activity of α-Glucosidase in Vitro

REN Weihe^{1, 2,},
LUO Longlong^{1, 2},
WANG Liping^{1, 2},
GAO Caiwen^{1, 2},
YU Jinfeng^{1, 2},
Peer Mohamed Abdul³,
DING Gongtao²,
CHEN Shien^1, ,

1.
School of Life Sciences and Engineering, Northwestern University of Nationalities, Lanzhou 730124, China
2.
Northwest University for Nationalities Biological Research Center, China-Malaysia National Joint Laboratory, Lanzhou 730124, China
3.
Faculty of Engineering and Built Environment, University Kebangsaan Malaysia, Bangi Selangor 43600, Malaysia

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Received Date: July 06, 2021
Available Online: January 07, 2022

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Abstract

Abstract

In this study, univariate and Box-Behnken response surface experiments were used to optimize the polyphenol extraction process of Rhodiola rosea. Then, a α-glucosidase inhibition system was constructed in vitro to study the inhibition activity of Rhodiola rosea polyphenols. At the same time, the type of inhibition was judged by enzyme inhibition kinetics. The univariate and response surface results showed that the optimal extraction process conditions were: Ethanol concentration of 71%, feed-to-liquid ratio 1:40, ultrasonic power 320 W, ultrasonic temperature 55 °C. Under this condition, the yield of Rhodiola rosea polyphenol extraction could reach 11.45%. Rhodiola rosea polyphenols had certain inhibitory activities on α-glucosidase, and the inhibition ability was quantitatively effective. Half inhibition concentration (IC₅₀) was 2.83 mg/mL, lower than the positive control acarbose (3.36 mg/mL of IC₅₀). When Rhodiola rosea polyphenol concentration was 50 mg/mL, the inhibition rate could reach 97.86%. Enzyme inhibitory kinetic studies showed that Rhodiola rosea polyphenols were reversible hybrid inhibitory types of α-glucosidase. As the concentration of the inhibitor increased, the maximum reaction rate V_max decreased and the Michael's constant K_m increased, which belonged to the competitive and non-competitive mixed types. This optimization test was effective and feasible, and the extracted Rhodiola rosea polyphenols had good inhibitory activity against α-glucosidase in vitro.
- Rhodiola rosea polyphenols,
- response surface,
- α-glucosidase,
- enzyme inhibition kinetics

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References

[1]	BUSE J B, WEXLER D J, TSAPAS A, et al. 2019 update to: Management of hyperglycemia in type 2 diabetes, 2018. A consensus report by the american diabetes association (ADA) and the European association for the study of diabetes (EASD)[J]. Diabetes Care,2020,43(2):487. doi: 10.2337/dci19-0066
[2]	WAN J X, LIM G, LEE J, et al. Inhibitory effect of phloroglucinolon α-glucosidase: Kinetics and molecular dynamics simulationintegration study[J]. Int J Bio Macromol,2019,124:771. doi: 10.1016/j.ijbiomac.2018.11.268
[3]	赵艳, 刘丽娟, 王白娟, 等. 雪胆多酚抗氧化作用及对α-葡萄糖苷酶抑制作用研究[J]. 西南农业学报,2021,34(5):984−991. [ZHAO Yan, LIU Lijuan, WANG Baijuan, et al. Study on the antioxidant effects of ferric polyphenols and inhibitions of α-glucosidenases[J]. Journal of Southwest Agricultural Sciences,2021,34(5):984−991.
[4]	SHOBANA S, SREERAMA Y N, MALLESHI N G. Composition and enzyme inhibitory properties of finger millet (Eleusine coracana L.) seed coat phenolics: Mode of inhibition of α-glucosidase and pancreatic amylase[J]. Food Chem,2009,115(4):1268−1273. doi: 10.1016/j.foodchem.2009.01.042
[5]	黄晓辰, 马金魁, 梁乐欣. 凤凰单枞多酚提取工艺优化及其体外抗氧化和α-葡萄糖苷酶抑制活性研究[J]. 食品工业科技,2020,41(9):88−94. [HUANG Xiaochen, MA Jinkui, LIANG Lexin. Optimization of the extraction process of phoenix Dancong polyphenols and its in vitro antioxidant and α-glucosidase inhibitory activities[J]. Science and Technology of Food Industry,2020,41(9):88−94.
[6]	宋菲, 陈开健, 唐敏敏, 等. 槟榔多酚提取物对α-葡萄糖苷酶活性的抑制动力学研究[J]. 热带作物学报,2021,42(5):1455−1461. [SONG Fei, CHEN Kaijian, TANG Minmin, et al. Inhibition kinetics of betel nut polyphenol extract on α-glucosidase activity[J]. Acta Tropical Crops,2021,42(5):1455−1461. doi: 10.3969/j.issn.1000-2561.2021.05.035
[7]	ZHOU J T, XU L, CHEN Y Y, et al. Chemical constituents from the Rhodiola genus plants[J]. J Chin Pharm Sci,2014,23(7):433−445.
[8]	吕秀梅, 范芳芳, 文检, 等. HPLC法同时测定3种红景天药材中5种化学成分的含量[J]. 中国药房,2018,29(18):2515−2519. [LV Xiumei, FAN Fangfang, WEN Jian, et al. Simultaneous determination of 5 chemical components in 3 Rhodiola medicinal materials by HPLC[J]. China Pharmacy,2018,29(18):2515−2519. doi: 10.6039/j.issn.1001-0408.2018.18.16
[9]	王艺博, 王峰, 肖智勇, 等. 大株红景天胶囊提高小鼠耐常压缺氧能力和抗疲劳作用研究[J]. 中国药理学与毒理学杂志: 1−8[2021-06-17]. http://kns.cnki.net/kcms/detail/11.1155.R.20210601.1708.004.html. WANG Yibo, WANG Feng, XIAO Zhiyong, et al. Research on the effect of Dazhu Rhodiola capsules on improving the ability of tolerance to atmospheric hypoxia and anti-fatigue in mice[J]. Chinese Journal of Pharmacology and Toxicology: 1−8[2021-06-17]. http://kns.cnki.net/kcms/detail/11.1155.R.20210601.1708.004.html.
[10]	ASYAKINA L, SUKHIKH S, IVANOVA S, et al. Determination of the qualitative composition of biologically-active substances of extracts of in vitro callus, cell suspension, and root cultures of the medicinal plant Rhodiola rosea[J]. Biomolecules,2021,11(3):365. doi: 10.3390/biom11030365
[11]	CHEN T, YAO L, KE D, et al. Treatment with Rodiola crenulata root extract ameliorates insulin resistance in fructose-fed rats by modulating sarcolemmal and intracellular fatty acid translocase/CD36 redistribution in skeletal muscle[J]. BMC Complement Altern Med,2016,16(1):1−10.
[12]	SHAN S Q, MENG L S, SUN Z, et al. Salidroside from Rhodiola rosea L. attenuates diabetic nephropathy in STZ induced diabetic rats via anti-oxidative stress, anti-inflammation, and inhibiting TGF-β1/Smad pathway[J]. Journal of Functional Foods,2021,77(2):104329.
[13]	吴梦. 红景天有效成分的提取及分离纯化[D]. 上海: 上海应用技术大学, 2019. WU Meng. Extraction, separation and purification of the effective components of Rhodiola rosea[D]. Shanghai: Shanghai University of Applied Sciences, 2019.
[14]	敖宇涛, 关琪轩, 丁雪, 等. 高山红景天总多酚提取工艺及其分散片制备[J]. 延边大学学报(自然科学版),2016,42(2):173−176. [AO Yutao, GUAN Qixuan, DING Xue, et al. Extraction technology of total polyphenols of Rhodiola rosea and preparation of dispersible tablets[J]. Journal of Yanbian University (Natural Science Edition),2016,42(2):173−176.
[15]	彭国岗, 白晓莉, 段如敏, 等. 超临界二氧化碳萃取红景天多酚及在卷烟中的应用[J]. 食品工业,2014,35(11):197−201. [PENG Guogang, BAI Xiaoli, DUAN Rumin, et al. Supercritical carbon dioxide extraction of Rhodiola polyphenols and its application in cigarettes[J]. Food Industry,2014,35(11):197−201.
[16]	PAOLA S, FEDERICA T, PAOLA L, et al. Rhodiola rosea, a protective antioxidant for intense physical exercise: An in vitro study[J]. Journal of Functional Foods,2018,48:27−36. doi: 10.1016/j.jff.2018.06.022
[17]	程安玮, 王文亮, 陈相艳, 等. 红景天多酚的提取及抗氧化活性研究[J]. 山东农业科学,2012,44(9):115−117. [CHENG Anwei, WANG Wenliang, CHEN Xiangyan, et al. Study on the extraction and antioxidant activity of Rhodiola rosea polyphenols[J]. Shandong Agricultural Sciences,2012,44(9):115−117. doi: 10.3969/j.issn.1001-4942.2012.09.036
[18]	海仁古丽买买提, 刘展. 海力茜陶尔大洪芜菁酸多酚的提取工艺研究[J]. 食晶安全质量检测学报,2019,10(23):7926−7933. [HAIREN G, LIU Z. Research on the extraction process of turnip acid polyphenols from Hailiqin Taoer Dahong[J]. Journal of Food Safety and Quality Inspection,2019,10(23):7926−7933.
[19]	USMAN, GHANI, MOHAMMAD, et al. Natural flavonoid α-glucosidase inhibitors from Retama raetam: Enzyme inhibition and molecular docking reveal important interactions with the enzyme active site[J]. Bioorganic Chemistry,2019,87:736−742. doi: 10.1016/j.bioorg.2019.03.079
[20]	宋慧, 成军. 槊皮多酚的乙醇提取及抗氧化性研究[J]. 徐州工程学院学报(自然科学版),2019,34(4):28−31. [SONG Hui, CHENG Jun. Study on ethanol extraction and antioxidant properties of polyphenols from chinensis bark[J]. Journal of Xuzhou Institute of Technology (Natural Science Edition),2019,34(4):28−31.
[21]	矫馨瑶, 李恩惠, 王月华, 等. 蓝莓多酚稳定性及热降解动力学研究[J]. 中国食品学报,2018,18(1):81−87. [JIAO Xinyao, LI Enhui, WANG Yuehua, et al. Study on stability and thermal degradation kinetics of blueberry polyphenols[J]. Chinese Journal of Food Science,2018,18(1):81−87.
[22]	林宝妹, 邱珊莲, 郑开斌, 等. 嘉宝果叶片总多酚提取工艺优化及其体外降糖活性[J]. 福建农业学报,2019,34(5):587−594. [LIN Baomei, QIU Shanlian, ZHENG Kaibin, et al. Optimization of extraction process of total polyphenols from Jiabao fruit leaves and their in vitro hypoglycemic activity[J]. Fujian Journal of Agricultural Sciences,2019,34(5):587−594.
[23]	李曌, 李宏强, 陈国刚, 等. 溶剂类型对葡萄籽多酚提取及抗氧化活性的影响[J]. 食品工业,2016,37(12):122−127. [LI Zhao, LI Hongqiang, CHEN Guogang, et al. Effects of solvent types on the extraction and antioxidant activity of grape seed polyphenols[J]. Food Industry,2016,37(12):122−127.
[24]	陈小平, 齐昆青, 程团结, 等. 维格列汀与阿卡波糖对2型糖尿病患者降血糖的临床疗效比较[J]. 河南医学研究,2013(2):208−210. [CHEN Xiaoping, QI Kunqing, CHENG Tuanjie, et al. Comparison of the clinical efficacy of vildagliptin and acarbose in lowering blood sugar in patients with type 2 diabetes[J]. Henan Medical Research,2013(2):208−210.
[25]	董琦, 谭亮, 胡娜, 等. 响应面法优化藜麦麸皮皂苷最佳提取工艺及其α-葡萄糖苷酶抑制活性[J]. 天然产物研究与开发,2021,33(6):935−942. [DONG Qi, TAN Liang, HU Na, et al. Optimization of the optimal extraction process of quinoa bran saponin and its α-glucosidase inhibitory activity by response surface methodology[J]. Natural Products Research and Development,2021,33(6):935−942.
[26]	田丽梅, 王旻, 陈卫. 枸杞多糖对α-葡萄糖苷酶的抑制作用[J]. 华西药学杂志,2006(2):131−133. [TIAN Limei, WANG Min, CHEN Wei. Inhibition of Lycium barbarum polysaccharides on α-glucosidase[J]. West China Journal of Pharmaceuticals,2006(2):131−133. doi: 10.3969/j.issn.1006-0103.2006.02.008
[27]	肖会芝. 天然多酚对胰蛋白酶和α-葡萄糖苷酶抑制机制的研究[D]. 重庆: 重庆大学, 2016 XIAO Huizhi. Study on the inhibitory mechanism of natural polyphenols on trypsin and α-glucosidase[D]. Chongqing: Chongqing University, 2016.
[28]	杨文康, 李超, 喻柯柯, 等. 罗汉果皂甙粗提物的α-葡萄糖苷酶体外抑制活性研究[J]. 食品工业科技,2016,37(24):111−115. [YANG Wenkang, LI Chao, YU Keke, et al. In vitro inhibitory activity of α-glucosidase of crude extract of mogrosides[J]. Science and Technology of Food Industry,2016,37(24):111−115.

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