Optimization Extraction Processing,Antioxidant Activity and Inhibition of α-Glucosidase Activity of Total Polyphenols from Leaves of Passiflora edulis

Objective:To sduty the extraction process and bioactivity of polyphenols from leaves of Passiflora edulis leaves. Methods:Extraction content of polyphenols from Passiflora edulis leaves was carried out by microwave-assisted method. The effects of ethanol volume fraction,microwave power,microwave time and ratio of material to liquid on the extraction of polyphenols were investigated. The orthogonal design was used to optimize the extraction process of polyphenol on the basis of single factor experiment. The antioxidant activity was evaluated by three kinds of free radical scavenging assays and reducing power assay. And the inhibitory effect on α-glucosidase was determined. Results:The optimum conditions were as follows:Alcohol volume fraction 60%,microwave power 400 W,microwave time 20 min,ratio of material to liquid 1:40 g/mL,and the total polyphenols yield was up to(2.200±0.015) mg/g. Conclusion:The polyphenols extracted from the leaves of Passiflora edulis revealed good antioxidant activity and inhibition of α-glucosidase.