Inhibition of <i>α</i>-Glucosidase Activity and Regulation of Blood Glucose by Methanol Extracts from <i>Amomum tsao-ko</i>

XIE Libin; CHEN Jia; JU Huidong; HU Lianxia; HAN Aiyun; YU Longquan

doi:10.13386/j.issn1002-0306.2021080077

Volume 43 Issue 8

Apr. 2022

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Science and Technology of Food Industry > 2022 > 43(8): 382-388. > DOI: 10.13386/j.issn1002-0306.2021080077

XIE Libin, CHEN Jia, JU Huidong, et al. Inhibition of α-Glucosidase Activity and Regulation of Blood Glucose by Methanol Extracts from Amomum tsao-ko[J]. Science and Technology of Food Industry, 2022, 43(8): 382−388. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021080077.

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Inhibition of α-Glucosidase Activity and Regulation of Blood Glucose by Methanol Extracts from Amomum tsao-ko

School of Chemical Engineering, Shijiazhuang University, Shijiazhuang 050035, China

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Received Date: August 05, 2021
Available Online: February 18, 2022

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Abstract

Abstract

Objective: To investigate α-glucosidase inhibitory and high blood glucose regulation of methanol extracts from Amomum tsao-ko in aged mice. Methods: Using 4-N-trophenyl-α-D-glu-copyranoside(PNPG) method with acarbose as positive control, α-glucosidase inhibition test in vitro was carried out to evaluate α-glucosidase inhibition rate of methanol extract from Amomum tsao-ko. The mouse model of abnormal blood glucose was established by feeding mice with high-fat diet. On the basis of high-fat diet, 100 and 200 mg/kg of methanol extracts from Amomum tsao-ko were added for 6 weeks to evaluate the effects of methanol extracts from Amomum tsao-ko on fasting blood glucose, glucose tolerance and insulin resistance in mice. Results: When the concentration of methanol extracts solution was 500 mg/L, the inhibition rate of α-glucosidase reached 63.72%. Half inhibitory concentration (IC₅₀) of methanol extract from Amomum tsao-ko on α-glucosidase was 0.145 mg/mL. The fasting blood glucose of mice in the herb group began to decrease and maintain stability after 2 weeks of intervention by intragastric administration of low and high dose methanol extracts of Amomum tsao-ko groups. The area under the curve (AUC) values of glucose tolerance curve in low and high dose methanol extracts of Amomum tsao-ko groups after 6 weeks intervention were lower than that of model group (P<0.05). Conclusion: Methanol extracts of Amomum tsao-ko could obviously inhibit α-glucosidase activity and improve glucose tolerance in mice.
- Amomum tsao-ko,
- methanol extracts,
- α-glucosidase,
- blood glucose

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