Screening and Mechanism of Inhibiting <i>α</i>-Glucosidase Activity Fraction from <i>Sparassis crispa</i>

WU Yong; WANG Zeyu; ZHOU Na; ZHAO Fu; ZHAO Jiashan; LI Yunye; WANG Xin; LI Jinjie

doi:10.13386/j.issn1002-0306.2021030320

Volume 42 Issue 22

Nov. 2021

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Science and Technology of Food Industry > 2021 > 42(22): 93-98. > DOI: 10.13386/j.issn1002-0306.2021030320

WU Yong, WANG Zeyu, ZHOU Na, et al. Screening and Mechanism of Inhibiting α-Glucosidase Activity Fraction from Sparassis crispa[J]. Science and Technology of Food Industry, 2021, 42(22): 93−98. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021030320.

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Screening and Mechanism of Inhibiting α-Glucosidase Activity Fraction from Sparassis crispa

Beijing Key Laboratory of Bioactive Substances and Functional Foods, Beijing Union University, Beijing 100191, China

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Received Date: March 28, 2021
Available Online: September 13, 2021

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Abstract

Abstract

Under the guidance of inhibition α-glucosidase activity in vitro, the inhibition effect and mechanism of inhibiting α-glucosidase activity of different polar components obtained from Sparassis crispa were investigated. The extracts of S. crispa were obtained by the ultrasonic-assisted extraction methods with two different ethanol concentrations. The different polar components were obtained by the extraction and the macroporous adsorption resin. All of these fractions were screened by the α-glucosidase inhibitory activity in vitro, and the active fractions were obtained. Half maximal inhibitory concentration(IC₅₀) was calculated, and the mechanism of action was investigated by enzymatic kinetics and Lineweaver-Burk methods. The results showed that the inhibition of α-glucosidase activity was strongest in the 60% ethanol eluting fraction of the extract of 50% ethanol, with IC₅₀ value of 0.0927±0.0600 mg/mL, which was equivalent to the activity of acarbose(IC₅₀: 0.0795±0.0200 mg/mL). It was competitive and non-competitive mixed inhibition type. This study identified the avtivity fraction of inhibiting α-glucosidase in the extract of S. crispa, and discussed the mechanism of action. It would provide a theoretical and scientific basis for developing hypoglycemic products for S. crispa in the future.
- Sparassis crispa,
- α-glucosidase,
- extraction,
- separation and purification,
- macroreticular resin

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