Extraction technology of collagen in Lophius litulon bone

In order to understand the basic composition and amino acid composition of the fish bone and to improve the extraction rate of collagen in Lophius litulon bone,the low molecular weight collagen was prepared by enzymolysis.On the basis of single factor,orthogonal experiment was used to optimize the extraction process of collagen.The results showed that the water,protein,fat,ash and calcium contents of the fish were 76.26%,13.28%,0.0025%,12.32% and 6.22% respectively.The total amino acid content of the fish bone was 76.57%,in which the Essential amino acid was 31.28%,the flavor amino acid was 16.94%,the hydroxyproline content was 3.68%.The optimization of collagen extraction conditions were as follows:extraction time 4 h,enzyme content 6%,temperature 40 ℃,pH1.Under this condition,the content of collagen was 43.06%,and the degree of hydrolysis was 27.08%.The collagen content of molecular weight below 30 kDa accounted for 20.65% of the total content.The experiment provided a theoretical and technical basis for further research and development of collagen and its related products.