Optimization of ultrasonic-assisted ethanol extraction of total flavonoids from Pinus koraiensis needles by response surface methodology

The aim of this paper was to extract the total flavonoids from Xiaoxing’anling Pinus koraiensis needles by ultrasonic assisted ethanol extraction method,and to achieve the purpose of rational utilization of the Northeast Pinus koraiensis needles. The ratio of material to liquid,volume fraction of ethanol,ultrasonic temperature,ultrasonic time were set as the main influencing factors,based on the single factor experiment,the total flavonoid yield was taken as the object of study,Box-Behnken center combination design and response surface methodology were used to optimize the extraction process of total flavonoids from Pinus koraiensis needles. The results showed that when the ratio of material to liquid was 1:52 g/mL,the volume fraction of ethanol was 51%,the ultrasonic temperature was 76 ℃ and the time was 103 min,the yield of total flavonoids was about 5.82%.The process conditions are reasonable and reliable,which provides a theoretical basis for industrial production and extraction.