Optimization of extraction process of total flavonoids from the pericarp and episperm of Fenghuang Dancong tea seed

In order to optimize the traditional ethanol extraction of total flavonoids from the pericarp and episperm of Fenghuang Dancong tea seed. The effects of 4 process parameters(including ethanol concentration,material-to-liquid ratio,temperature and extraction time)were investigated by single factor test and orthogonal array design(four factors and three levels). The results showed:The optimized conditions for extracting total flavonoids from the pericarp of Fenghuang Dancong tea trees were as follows:Material-to-liquid ratio 1∶30 (g/mL),50% ethanol concentration,temperature 70 ℃and extraction duration 120 min.Under the optimized conditions,the predicted extraction yield of total flavonoids from the pericarp was(7.56±0.04) mg/g.The optimal technical conditions were mixing the episperm of the Fenghunag Dancong tea trees,with 50% ethanol concentration on the ratio of 1∶30 (g/mL)at 80 ℃ for 90 min. Under this optimized conditions,the extraction rate was reaching(9.72±0.05) mg/g.