Improvement effects and mechanism research of polyphenol extracts from Kudingcha on carbon tetrachloride induced hepatic damage in mice

The improvement effects of polyphenol extracts of kudingcha(PEK)on hepatic damage in mice were determined in this study. The mice were fed with PEK by gavage,and through the mice were induced hepatic damage using CCl₄ by intraperitoneal injection,the serum and mRNA expression of hepatic tissue in mice were measured. The results showed that the polyphenol contents of PEK was 71.3%. PEK could reduce the liver index of CCl₄-treated mice as compared to the control group,meanwhile,and PEK also could significantly reduce the serum AST,ALT,ALP,TG,TC,BUN,NO,MDA levels and raise SOD,CAT,GSH-Px levels(p<0.05). PEK also could significantly decrease serum cytokine IL-6,IL-12,TNF-α and IFN-γ levels in gastric injury mice(p<0.05). Pathological observation also showed that PEK could effectively reduce the hepatic tissue destruction and hepatocellular necrosis caused by CCl₄. The RT-PCR experiment results showed that PEK could significantly increase Mn-SOD,Gu/Zn-SOD,CAT,GSH-Px mRNA expressions and decrease COX-2,IL-1β,TNF-α expressions of hepatic tissues in mice compared to the mice in control group(p<0.05). It could be seen that PEK is a kind of active component with improving liver injury,therefore it has a good application prospect.